Advertisement

Prevalence of Human Papilloma Virus Infection in Cervical Cancer Patients from Western Region of India

  • Ashi R. Thobias
  • Kinjal A. Patel
  • Riddhi Gokani
  • Chetna Parekh
  • Ava Desai
  • Jayendra B. Patel
  • Prabhudas S. PatelEmail author
Original Article
  • 8 Downloads

Abstract

Background

Cervical cancer (CaCx) is a major malignancy affecting women in India. Human papilloma viruses (HPVs) have been associated with occurrence of cervical cancer. Hence, we aimed to evaluate cervical cancer burden associated with HPV infections.

Materials and Methods

DNA was isolated from 100 CaCx patients. PCR was performed using degenerative primers for HPV infections as well as type-specific primers for HPV 16 and HPV 18 types.

Results

In the present cohort, 87%, 89% and 26% cases showed HPV positivity when analyzed using degenerative primers MY 09/11, GP 5+/6+ and CP I/II, respectively. While HPV 16 and HPV 18 positivity was observed in 56% and 15% cases, respectively, whereas 6% of the cases showed co-infection with both these types. All cervical cancer cases in the cohort were positive for HPV infection when analyzed by all the primer sets.

Conclusion

The data depict significant prevalence of HR-HPV types HPV 16 and HPV 18 while also showing high prevalence of HPV infection on detection with the degenerative primer sets. Thus, suggesting that investigation of HPV infection using multiple primers sets is very crucial to avoid false-negative results.

Keywords

Cervical cancer HPV 16 HPV 18 HPV Western India 

Notes

Acknowledgements

The authors would like to extend their gratitude toward The Gujarat Cancer Society and The Gujarat Cancer Research Institute for providing the funds for the Project (EC/08/14).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Akarolo-Anthony SN, Famooto AO, Dareng EO, Olaniyan OB, Offiong R, Wheeler CM, Adebamowo CA. Age-specific prevalence of human papilloma virus infection among Nigerian women. BMC Public Health. 2014;14(1):656.CrossRefGoogle Scholar
  2. 2.
    Baskaran K, Kumar PK, Karunanithi S, Sethupathy S, Thamaraiselvi B, Swaruparani S. Detection of high-risk human papilloma viruses in the prevention of cervical cancer in india. Asian Pac J Cancer Prev. 2015;16(18):818.Google Scholar
  3. 3.
    Basu P, Roychowdhury S, Bafna UD, Chaudhury S, Kothari S, Sekhon R, Saranath D, Biswas S, Gronn P, Silva I, Siddiqi M. Human papillomavirus genotype distribution in cervical cancer in India: results from a multi-center study. Asian Pac J Cancer Prev. 2009;10(1):27–34.PubMedGoogle Scholar
  4. 4.
    Bhatla N, Dar L, Patro AR, Kriplani A, Gulati A, Verma K, Broor S, Shah KV, Gravitt PE. Human papillomavirus type distribution in cervical cancer in Delhi, India. Int J Gynecol Pathol. 2006;25(4):398–402.CrossRefGoogle Scholar
  5. 5.
    Bhatla N, Lal N, Bao YP, Ng T, Qiao YL. A meta-analysis of human papillomavirus type-distribution in women from South Asia: implications for vaccination. Vaccine. 2008;26(23):2811–7.CrossRefGoogle Scholar
  6. 6.
    Bhattacharya A, Sen S, Mandal P, Saha SS, Sarkar S, Pathak OP, Biswas L, Roy J, Banerjee R, Chowdhury RR, Pal M. Prevalence and age-wise distribution of human papillomavirus type 16/18 infections among hospital screened women of a peri-urban area in West Bengal: impact of socio-demographic factors. Cancer Epidemiol. 2018;30(54):31–7.CrossRefGoogle Scholar
  7. 7.
    Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A, Global Cancer Statistics 2018. GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68(6):394–424.CrossRefGoogle Scholar
  8. 8.
    Castle PE, Schiffman M, Gravitt PE, Kendall H, Fishman S, Dong H, Hildesheim A, Herrero R, Bratti MC, Sherman ME, Lorincz A. Comparisons of HPV DNA detection by MY09/11 PCR methods. J Med Virol. 2002;68(3):417–23.CrossRefGoogle Scholar
  9. 9.
    Chao A, Hsu KH, Lai CH, Huang HJ, Hsueh S, Lin SR, Jung SM, Chao FY, Huang SL, Huang CC, Yang JE. Cervical cancer screening program integrating Pap smear and HPV DNA testing: a population-based study. Int J Cancer. 2008;122(12):2835–41.CrossRefGoogle Scholar
  10. 10.
    Chatterjee S, Chattopadhyay A, Samanta L, Panigrahi P. HPV and cervical cancer epidemiology—current status of HPV vaccination in India. Asian Pac J Cancer Prev. 2016;17(8):3663–73.PubMedGoogle Scholar
  11. 11.
    Corden SA, Sant-Cassia LJ, Easton AJ, Morris AG. The integration of HPV-18 DNA in cervical carcinoma. Mol Pathol. 1999;52(5):275.CrossRefGoogle Scholar
  12. 12.
    Daniel B, Mukherjee G, Seshadri L, Vallikad E, Krishna S. Changes in the physical state and expression of human papillomavirus type 16 in the progression of cervical intraepithelial neoplasia lesions analysed by PCR. J Gen Virol. 1995;76(10):2589–93.CrossRefGoogle Scholar
  13. 13.
    Daniel B, Rangarajan A, Mukherjee G, Vallikad E, Krishna S. The link between integration and expression of human papillomavirus type 16 genomes and cellular changes in the evolution of cervical intraepithelial neoplastic lesions. J Gen Virol. 1997;78(5):1095–101.CrossRefGoogle Scholar
  14. 14.
    Das BC, Gopalkrishna V, Hedau S, Katiyar S. Cancer of the uterine cervix and human papillomavirus infection. Curr Sci Bangalore. 2000;78(1):52–63.Google Scholar
  15. 15.
    de Martel C, Plummer M, Vignat J, Franceschi S. Worldwide burden of cancer attributable to HPV by site, country and HPV type. Int J Cancer. 2017;141(4):664–70.CrossRefGoogle Scholar
  16. 16.
    Depuydt CE, Boulet GA, Horvath CA, Benoy IH, Vereecken AJ, Bogers JJ. Comparison of MY09/11 consensus PCR and type-specific PCRs in the detection of oncogenic HPV types. J Cell Mol Med. 2007;11(4):881–91.CrossRefGoogle Scholar
  17. 17.
    Franceschi S. The IARC commitment to cancer prevention: the example of papillomavirus and cervical cancer. In: Senn HJ, Morant R, editors. Tumor prevention and genetics III. Berlin: Springer; 2005. pp. 277–97.CrossRefGoogle Scholar
  18. 18.
    Kaarthigeyan K. Cervical cancer in India and HPV vaccination. Indian J Med Paediatr Oncol. 2012;33(1):7.CrossRefGoogle Scholar
  19. 19.
    Karlsen F, Kalantari M, Jenkins A, Pettersen E, Kristensen G, Holm R, Johansson B, Hagmar B. Use of multiple PCR primer sets for optimal detection of human papillomavirus. J Clin Microbiol. 1996;34(9):2095–100.PubMedPubMedCentralGoogle Scholar
  20. 20.
    Park JY, Lee KH, Dong SM, Kang S, Park SY, Seo SS. The association of pre-conization high-risk HPV load and the persistence of HPV infection and persistence/recurrence of cervical intraepithelial neoplasia after conization. Gynecol Oncol. 2008;108(3):549–54.CrossRefGoogle Scholar
  21. 21.
    Patel KR, Vajaria BN, Begum R, Desai A, Patel JB, Shah FD, Shukla SN, Patel PS. Prevalence of high-risk human papillomavirus type 16 and 18 in oral and cervical cancers in population from Gujarat, West India. J Oral Pathol Med. 2014;43(4):293–7.CrossRefGoogle Scholar
  22. 22.
    Qu W, Jiang G, Cruz Y, Chang CJ, Ho GY, Klein RS, Burk RD. PCR detection of human papillomavirus: comparison between MY09/MY11 and GP5+/GP6+ primer systems. J Clin Microbiol. 1997;35(6):1304–10.PubMedPubMedCentralGoogle Scholar
  23. 23.
    Sathish N, Abraham P, Peedicayil A, Sridharan G, John S, Shaji RV, Chandy G. HPV DNA in plasma of patients with cervical carcinoma. J Clin Virol. 2004;31(3):204–9.CrossRefGoogle Scholar
  24. 24.
    Senapati R, Nayak B, Kar SK, Dwibedi B. HPV Genotypes distribution in Indian women with and without cervical carcinoma: implication for HPV vaccination program in Odisha, Eastern India. BMC Infect Dis. 2017;17(1):30.CrossRefGoogle Scholar
  25. 25.
    Sowjanya AP, Jain M, Poli UR, Padma S, Das M, Shah KV, Rao BN, Devi RR, Gravitt PE, Ramakrishna G. Prevalence and distribution of high-risk human papilloma virus (HPV) types in invasive squamous cell carcinoma of the cervix and in normal women in Andhra Pradesh, India. BMC Infect Dis. 2005;5(1):116.CrossRefGoogle Scholar
  26. 26.
    Szostek S, Klimek M, Zawilinska B, Kosz-Vnenchak M. Genotype-specific human papillomavirus detection in cervical smears. Statistics. 2008;53(54):74.Google Scholar
  27. 27.
    Uthayaruban R, Fernandopulle N. Establishment of a molecular diagnostic system for detecting human papillomavirus in clinical samples in Sri Lanka. Southeast Asian J Trop Med Public Health. 2011;42(6):1423–30.PubMedGoogle Scholar
  28. 28.
    Ziegert C, Wentzensen N, Vinokurova S, Kisseljov F, Einenkel J, Hoeckel M, von KnebelDoeberitz M. A comprehensive analysis of HPV integration loci in anogenital lesions combining transcript and genome-based amplification techniques. Oncogene. 2003;22(25):3977.CrossRefGoogle Scholar
  29. 29.
    ZurHausen H. Papillomaviruses in human cancers. Proc Assoc Am Physicians. 1999;111(6):581–7.CrossRefGoogle Scholar

Copyright information

© Association of Gynecologic Oncologists of India 2019

Authors and Affiliations

  • Ashi R. Thobias
    • 1
  • Kinjal A. Patel
    • 1
  • Riddhi Gokani
    • 1
  • Chetna Parekh
    • 2
  • Ava Desai
    • 2
  • Jayendra B. Patel
    • 1
  • Prabhudas S. Patel
    • 1
    • 3
    Email author
  1. 1.Molecular Oncology LaboratoryThe Gujarat Cancer and Research InstituteAsarwa, AhmedabadIndia
  2. 2.Department of Gynecological OncologyThe Gujarat Cancer and Research InstituteAsarwa, AhmedabadIndia
  3. 3.Cancer Biology DepartmentThe Gujarat Cancer and Research InstituteAsarwa, AhmedabadIndia

Personalised recommendations