Burn and Wound Healing Using Radiation Sterilized Human Amniotic Membrane and Centella asiatica Derived Gel: a Review

  • Md. Liakat Hossain
  • Md. Arifur Rahman
  • Ayesha Siddika
  • M. H. Adnan
  • Hafizur Rahman
  • Farzana Diba
  • Md. Zahid Hasan
  • S. M. AsaduzzamanEmail author


Burn injury is a common clinical concern that leads to morbidity and mortality, especially in the developing countries. Nowadays, it is a world-wide challenge for the patients due to the long-term hospital stay and undue side effects for microbial contamination of marketed burn dressing materials. So, scientists are trying hard to find out materials with fewer side effects, more cost-effective, and easily available to reduce the sufferings of burn patients. For this, researchers from all over the world are giving great focus on the biomaterials such as human amniotic membrane (AM) and various plant extracts. AM and Centella asiatica (CA), a medicinal plant, are individually used for burn and wound healing. AM heals quicker than any other wound healing components in the case of 1st- and 2nd-degree burn. Furthermore, CA contains various important alkaloids, phenolics, asiatic acid, madecassic acid, asiaticoside, and madecassoside. Among them, asiatic acid and madecassic acid exert anti-inflammatory, apoptotic effect, while asiaticoside and madecassoside stimulate collagen (I, II, III) synthesis, new blood vessel formation that ultimately helps to burn wound healing. Moreover, vimentin and α-SMA produced by CA also help in faster healing. Since burn patients suffer a lot of difficulties and complexities due to the slow rate of epithelization, a time-consuming process and several side effects of the available burn-healing agents, this review focuses on the synergistic and complementary effects of AM and CA for faster burn and wound healing.

Lay Summary

Burn injury is increasing day by day worldwide and this phenomenon is suspected to be persistent. Although treatment exists, it is expensive and takes a longer period to heal and sometimes shows adverse effects. Since AM and CA are available and widely used as burn and wound dressing material, a gel mixed with both of them may be used to boost up burn healing.


Burn and wound injury Amniotic membrane (AM) Centella asiatica (CA) The combined effect Faster healing 



amniotic membrane


Centella asiatica


silver sulfadiazine


Institute of Tissue Banking and Biomaterial Research


vascular endothelial growth factor


hepatocyte growth factor


transforming growth factor


human leukocyte antigen


differential regulation


mesenchymal stem cells


secretary leukocyte proteinase inhibitor


amniotic epithelial cells


amniotic mesenchymal cells



I would like to thank all the authors of this study.

Compliance with Ethical Standards

Competing Interests

The authors declare that they have no conflict of interest.

Human and Animal Rights and Informed Consent

The Institute of Tissue Banking and Biomaterial Research (ITBBR) performs its research and developmental activities with human tissue samples under the authority of the Bangladesh Atomic Energy Commission with the strong cooperation of the International Atomic Energy Commission (IAEA). Besides, two government bills had been passed entitled “Human Organ/Tissue Donation and Transplantation Act (5/1999)” and “Safe Blood Transfusion Act (12/2002)”. ITBBR strongly follows the guideline of IAEA regarding Tissue Banking set up, e.g., the American Association of Tissue Bank (AATB) and the European Association of Tissue Bank (EATB). And samples are collected at ITBBR getting informed consent from the patients.


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Copyright information

© The Regenerative Engineering Society 2019

Authors and Affiliations

  • Md. Liakat Hossain
    • 1
  • Md. Arifur Rahman
    • 2
  • Ayesha Siddika
    • 1
  • M. H. Adnan
    • 1
  • Hafizur Rahman
    • 2
  • Farzana Diba
    • 1
  • Md. Zahid Hasan
    • 1
  • S. M. Asaduzzaman
    • 1
    Email author
  1. 1.Institute of Tissue Banking and Biomaterial ResearchAtomic Energy Research Establishment (AERE)SavarBangladesh
  2. 2.Department of Biochemistry & Molecular BiologyJahangirnagar UniversitySavarBangladesh

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