Silicon-Substituted Hydroxyapatite Particles and Response of Adipose Stem Cells In Vitro
- 32 Downloads
Due to the similarity of synthetic hydroxyapatite (HA) to natural bone tissue, but, because of its low degradation rate, the current study focuses on silicon-substituted HA (Si-HA) synthesis, characterization, and biological evaluations. Si-HA was successfully prepared through sol-gel processing route and characterized using SEM, EDX, XRD, and FTIR. Si-HA particles were found to be non-cytotoxic following exposure to adipose stem cells (ADSCs). In fact, Si-HA particles showed a high level of matrix mineralization following prolonged and continuous exposure to ADSCs. It is suggested that the incorporation of Si in HA structure positively affects cellular behavior, associated with a higher degradation rate, and subsequently greater level of ionic product release from Si-HA particles.
Hydroxyapatite (HA) has long been applied as bone substitutes but its low degradation rate limits its application. One approach is the incorporation of silicon (Si) within HA structure. This study confirms that Si-substituted HA enhance stem cell proliferation and promote osteogenic differentiation. Hence, Si-HA could be utilized in composites, scaffolds, and coatings for bone-related disorders.
KeywordsSi-HA particles Bone tissue engineering Adipose stem cells
- 20.Kim HW, Kim HE, Salih V, Knowles JC. Hydroxyapatite and titania sol–gel composite coatings on titanium for hard tissue implants; mechanical and in vitro biological performance. J Biomed Mater Res B. 2005;72:1–8.Google Scholar
- 24.Alabdulkarim Y, Ghalimah B, Al-Otaibi M, Al-Jallad HF, Mekhael M, Willie B, et al. Recent advances in bone regeneration: the role of adipose tissue-derived stromal vascular fraction and mesenchymal stem cells. J Limb Lengthen Reconstr. 2017;3:4.Google Scholar
- 25.Reumann MK, Linnemann C, Aspera-Werz RH, Arnold S, Held M, Seeliger C, et al. Donor site location is critical for proliferation, stem cell capacity, and osteogenic differentiation of adipose mesenchymal stem/stromal cells: implications for bone tissue engineering. Int J Mol Sci. 2018;19.Google Scholar
- 33.Bang L, Long B, Othman R. Carbonate hydroxyapatite and silicon-substituted carbonate hydroxyapatite: synthesis, mechanical properties, and solubility evaluations. Sci World J. 2014:2014.Google Scholar
- 34.Porter AE, Botelho CM, Lopes MA, Santos JD, Best SM, Bonfield W. Ultrastructural comparison of dissolution and apatite precipitation on hydroxyapatite and silicon-substituted hydroxyapatite in vitro and in vivo. J Biomed Mater Res A. 2004;69:670–9.Google Scholar
- 42.Porter AE, Best SM, Bonfield W. Ultrastructural comparison of hydroxyapatite and silicon-substituted hydroxyapatite for biomedical applications. Biomed Mater Res A. 2004;68:133–41.Google Scholar