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Biochar amendment increases soil microbial biomass and plant growth and suppresses Fusarium wilt in tomato

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The use of biochar as a means of mitigating climate change and improving soil physical and chemical characteristics has been extensively studied over the last two decades. However, the effects of biochar on the soil microbiota and plant diseases, especially those caused by soilborne plant pathogens, have received little attention and are poorly understood. The objectives of this study were to evaluate the effects of biochar at different concentrations incorporated into two soils on the control of Fusarium wilt, tomato development and soil microbial activity. The severity of Fusarium wilt and microbial activities (microbial biomass nitrogen and alkaline phosphatase) were inversely proportional to the concentrations (0 to 5%, v/v) of biochar incorporated into the soil. The fresh root and dry shoot masses and stem diameter measures increased with the increase in biochar concentration. Biochar aqueous extract did not affect Fusarium mycelial growth, but microconidial germination was reduced with the increase in the concentration of biochar aqueous extract. The biochar used in the present study has the potential to increase soil microbial biomass, promote plant growth and reduce the severity of tomato Fusarium wilt.

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  1. ASTM (2013) American Society for Testing Materials ASTM D1762-84: standard test method for chemical analyses of wood charcoal. Available online at https://www.astm.org/Standards/D1762.htm

  2. Bailey VL, Fansler SJ, Smith JL, Bolton H Jr (2011) Reconciling apparent variability in effects of biochar amendment on soil enzyme activities by assay optimization. Soil Biology and Biochemistry 43:296–301

  3. Bataglia O, Teixeira J, Furlani P, Furlani A, Gallo J (1983) Métodos de análise química de plantas. Intituto Agronômico de Campinas, Campinas

  4. Berglund L, Deluca T, Zackrisson O (2004) Activated carbon amendments to soil alters nitrification rates in scots pine forests. Soil Biology and Biochemistry 36:2067–2073

  5. Bikbulatova S, Tahmasebi A, Zhang Z, Rish SK, Yu J (2018) Understanding water retention behavior and mechanism in bio-char. Fuel Processing Technology 169:101–111

  6. BRASIL (2017) Ministério de Minas e Energia. Balanço Energético Nacional 2017: ano base 2016. EPE, Rio de Janeiro

  7. Breazeale J (1906) Effect of certain solids upon the growth of seedlings in water cultures. Botanical Gazette 41:54–63

  8. Callaway RM, Aschehoug ET (2000) Invasive plants versus their new and old neighbors: a mechanism for exotic invasion. Science 290:521–523

  9. Chan KY, Van Zwieten L, Meszaros I, Downie A, Joseph S (2008) Agronomic values of greenwaste biochar as a soil amendment. Soil Research 45:629–634

  10. Cook RJ, Thomashow LS, Weller DM, Fujimoto D, Mazzola M, Bangera G, Kim D-S (1995) Molecular mechanisms of defense by rhizobacteria against root disease. Proceedings of the National Academy of Sciences 92:4197–4201

  11. Dempster D, Gleeson D, Solaiman ZI, Jones D, Murphy D (2012) Decreased soil microbial biomass and nitrogen mineralisation with Eucalyptus biochar addition to a coarse textured soil. Plant and Soil 354:311–324

  12. Ding Y, Liu Y-X, Wu W-X, Shi D-Z, Yang M, Zhong Z-K (2010) Evaluation of biochar effects on nitrogen retention and leaching in multi-layered soil columns. Water, Air, & Soil Pollution 213:47–55

  13. Dispenza V, De Pasquale C, Fascella G, Mammano M, Alonzo G (2016) Use of biochar as peat substitute for growing substrates of Euphorbia × lomi potted plants. Spanish Journal of Agricultural Research 14:1–11

  14. Du Z, Wang Y, Huang J, Lu N, Liu X, Lou Y, Zhang Q (2014) Consecutive biochar application alters soil enzyme activities in the winter wheat–growing season. Soil Science 179:75–83

  15. Elad Y, David DR, Harel YM, Borenshtein M, Kalifa HB, Silber A, Graber ER (2010) Induction of systemic resistance in plants by biochar, a soil-applied carbon sequestering agent. Phytopathology 100:913–921

  16. Elmer WH, Pignatello JJ (2011) Effect of biochar amendments on mycorrhizal associations and Fusarium crown and root rot of asparagus in replant soils. Plant Disease 95:960–966

  17. Ferreira DF (2011) Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia 35:1039–1042

  18. Gaskin JW, Steiner C, Harris K, Das KC, Bibens B (2008) Effect of low-temperature pyrolysis conditions on biochar for agricultural use. Transaction of the American Society of Agricultural and Biological Engineers 51:2061–2069

  19. Ghini R, Mendes M, Bettiol W (1998) Método de hidrólise de diacetato de fluoresceina (FDA) como indicador da atividade microbiana do solo e supressividade a Rhizoctonia solani. Summa Phytopathologica 24:239–242

  20. Gordon TR (2017) Fusarium oxysporum and the Fusarium wilt syndrome. Annual Review of Phytopathology 55:23–39

  21. Graber ER, Harel YM, Kolton M, Cytryn E, Silber A, David DR, Tsechansky L, Borenshtein M, Elad Y (2010) Biochar impact on development and productivity of pepper and tomato grown in fertigated soilless media. Plant and Soil 337:481–496

  22. Grossman JM, O'Neill BE, Tsai SM, Liang B, Neves E, Lehmann J, Thies JE (2010) Amazonian anthrosols support similar microbial communities that differ distinctly from those extant in adjacent, unmodifield soils the same mineralogy. Microbial Ecology 60:192–205

  23. He L, Zhong Z, Yang H (2016) Effects on soil quality of biochar and straw amendment in conjunction with chemical fertilizers. Journal of Integrative Agriculture 15:704–712

  24. Heck DW, Ghini R, Bettiol W (2019) Deciphering the suppressiveness of banana Fusarium wilt with organic residues. Applied Soil Ecology 138:47–60

  25. Hoitink HA, Fahy PC (1986) Basis for the control of soilborne plant pathogens with composts. Annual Review of Phytopathology 24:93–114

  26. IBI – International Biochar Initiative (2015) Standardized product definition and product testing guidelines for biochar that is used in soil (aka IBI Biochar Standards) Version 2.1, 2015. Available online at https://biochar-international.org/characterizationstandard/

  27. Jaiswal AK, Elad Y, Graber ER, Frenkel O (2014) Rhizoctonia solani suppression and plant growth promotion in cucumber as affected by biochar pyrolysis temperature, feedstock and concentration. Soil Biology and Biochemistry 69:110–118

  28. Jaiswal AK, Frenkel O, Elad Y, Lew B, Graber ER (2015) Non-monotonic influence of biochar dose on bean seedling growth and susceptibility to Rhizoctonia solani: the “shifted R max-effect”. Plant and Soil 395:125–140

  29. Jin H (2010) Characterization of microbial life colonizing biochar and biochar-amended soils. PhD Dissertation, Cornell University. Ithaca, NY, USA

  30. Jones JP (1991) Fusarium wilt. In: Jones JB, Jones JP, Stall RE, Zitter TA (eds) Compendium of tomato diseases. APS Press, St. Paul, p 15

  31. Kandeler E, Gerber H (1988) Short-term assay of soil urease activity using colorimetric determination of ammonium. Biology and Fertility of Soils 6:68–72

  32. Kim JS, Sparovek G, Longo RM, De Melo WJ, Crowley D (2007) Bacterial diversity of terra preta and pristine forest soil from the Western Amazon. Soil Biology and Biochemistry 39:684–690

  33. Kulmatiski A (2011) Changing soils to manage plant communities: activated carbon as a restoration tool in ex-arable fields. Restoration Ecology 19:102–110

  34. Kulmatiski A, Beard KH (2006) Activated carbon as a restoration tool: potential for control of invasive plants in abandoned agricultural fields. Restoration Ecology 14:251–257

  35. Laird DA (2008) The charcoal vision: a win–win–win scenario for simultaneously producing bioenergy, permanently sequestering carbon, while improving soil and water quality. Agronomy Journal 100:178–181

  36. Laird D, Fleming P, Wang B, Horton R, Karlen D (2010) Biochar impact on nutrient leaching from a Midwestern agricultural soil. Geoderma 158:436–442

  37. Lazarovits G, Conn K, Abbasi P, Tenuta M (2005) Understanding the mode of action of organic soil amendments provides the way for improved management of soilborne plant pathogens. Acta Horticulturae 698:215–224

  38. Lehmann J (2007) Bio-energy in the black. Frontiers in Ecology and the Environment 5:381–387

  39. Lehmann J, Joseph S (2009) Biochar for environmental management: an introduction. In: Lehmann J, Joseph S (eds) Biochar for environmental management: science and technology. Earthscan, London & Sterling, pp 1–9

  40. Lehmann J, Da Silva JP, Steiner C, Nehls T, Zech W, Glaser B (2003) Nutrient availability and leaching in an archaeological Anthrosol and a Ferralsol of the Central Amazon basin: fertilizer, manure and charcoal amendments. Plant and Soil 249:343–357

  41. Lehmann J, Gaunt J, Rondon M (2006) Bio-char sequestration in terrestrial ecosystems–a review. Mitigation and Adaptation Strategies for Global Change 11:403–427

  42. Lehmann J, Rillig MC, Thies J, Masiello CA, Hockaday WC, Crowley D (2011) Biochar effects on soil biota–a review. Soil Biology and Biochemistry 43:1812–1836

  43. Li Q, Lei Z, Song X, Zhang Z, Ying Y, Peng C (2018) Biochar amendment decreases soil microbial biomass and increases bacterial diversity in Moso bamboo (Phyllostachys edulis) plantations under simulated nitrogen deposition. Environmental Research Letters 13:044029

  44. Liang B, Lehmann J, Solomon D, Kinyangi J, Grossman J, O'Neill B, Skjemstad J, Thies J, Luizao F, Petersen J (2006) Black carbon increases cation exchange capacity in soils. Soil Science Society of America Journal 70:1719–1730

  45. Makoto K, Tamai Y, Kim Y, Koike T (2010) Buried charcoal layer and ectomycorrhizae cooperatively promote the growth of Larix gmelinii seedlings. Plant and Soil 327:143–152

  46. Nair RP (2014) Grand challenges in agroecology and land use systems. Frontiers in Environmental Science 2:1

  47. Nash SM, Snyder WC (1962) Quantitative estimations by plate counts of propagules of the bean root rot Fusarium in field soils. Phytopathology 52:567–572

  48. Novak JM, Busscher WJ, Laird DL, Ahmedna M, Watts DW, Niandou MA (2009) Impact of biochar amendment on fertility of a southeastern coastal plain soil. Soil Science 174:105–112

  49. O’Neill B, Grossman J, Tsai M, Gomes JE, Lehmann J, Peterson J, Neves E, Thies JE (2009) Bacterial community composition in Brazilian Anthrosols and adjacent soils characterized using culturing and molecular identification. Microbial Ecology 58:23–35

  50. Obia A, Mulder J, Hale SE, Nurida NL, Cornelissen G (2018) The potential of biochar in improving drainage, aeration and maize yields in heavy clay soils. PLoS One 13:e0196794

  51. Pietikäinen J, Kiikkilä O, Fritze H (2000) Charcoal as a habitat for microbes and its effect on the microbial community of the underlying humus. Oikos 89:231–242

  52. Postma J, Clematis F, Nijhuis EH, Someus E (2013) Efficacy of four phosphate-mobilizing bacteria applied with an animal bone charcoal formulation in controlling Pythium aphanidermatum and Fusarium oxysporum f. sp. radicis lycopersici in tomato. Biological Control 67:284–291

  53. Prendergast-Miller MT, Duvall M, Sohi SP (2011) Localisation of nitrate in the rhizosphere of biochar-amended soils. Soil Biology and Biochemistry 43:2243–2246

  54. Raij BV, Cantarella H, Quaggio JA, Furlani AM (Eds) (1996) Recomendação de adubação e calagem para o estado de São Paulo. Boletim Técnico IAC n. 100. Campinas, SP. IAC

  55. Reis A, Boiteux LS (2007) Outbreak of Fusarium oxysporum f. sp. lycopersici race 3 in commercial fresh-market tomato fields in Rio de Janeiro State, Brazil. Horticultura Brasileira 25:451–454

  56. Reis A, Costa H, Boiteux LS, Lopes CA (2005) First report of Fusarium oxysporum f. sp. lycopersici race 3 on tomato in Brazil. Fitopatologia Brasileira 30:426–428

  57. Scharfy D, Boccali N, Stucki M (2017) Clean technologies in agriculture—how to prioritise measures? Sustainability 9:1303

  58. Shaner G, Finney RE (1977) The effect of nitrogen fertilization on the expression of slow-mildewing resistance in Knox wheat. Phytopathology 67:1051–1056

  59. Smith P, Gregory PJ (2013) Climate change and sustainable food production. Proceedings of the Nutrition Society. 72:21–28

  60. Sohi SP, Krull E, Lopez-Capel E, Bol R (2010) A review of biochar and its use and function in soil. In: Sparks DL (ed) Advances in agronomy, vol 105. Academic Press, Burllington, pp 47–82

  61. Steiner C, Glaser B, Geraldis-Teixeira W, Lehmann J, Blum WE, Zech W (2008) Nitrogen retention and plant uptake on a highly weathered central amazonian Ferralsol amended with compost and charcoal. Journal of Plant Nutrition and Soil Science 171:893–899

  62. Tabatabai M, Bremner J (1969) Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biology and Biochemistry 1:301–307

  63. Tedesco MJ, Gianello C, Bissani CA, Bohen H, Volkweiss SJ (1995) Análise de solo, plantas e outros materiais. Universidade Federal Rio Grande do Sul, Porto Alegre p. 174

  64. Tokeshi H, Galli F (1966) Variabilidade de Fusarium oxysporum f. sp. lycopersici (Wr) Sny & Hans em São Paulo. Anais da Escola Superior de Agricultura Luiz de Queiroz 23:195–209

  65. Van Zwieten L, Kimber S, Morris S, Chan K, Downie A, Rust J, Joseph S, Cowie A (2010) Effects of biochar from slow pyrolysis of papermill waste on agronomic performance and soil fertility. Plant and Soil 327:235–246

  66. Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry 19:703–707

  67. Wang D, Fonte SJ, Parikh SJ, Six J, Scow KM (2017) Biochar additions can enhance soil structure and the physical stabilization of C in aggregates. Geoderma 303:110–117

  68. Warnock DD, Lehmann J, Kuyper TW, Rillig MC (2007) Mycorrhizal responses to biochar in soil–concepts and mechanisms. Plant and Soil 300:9–20

  69. Windstam S, Nelson EB (2008) Temporal release of fatty acids and sugars in the spermosphere: impacts on Enterobacter cloacae-induced biological control. Applied and Environmental Microbiology 74:4292–4299

  70. Woolf D (2008) Biochar as a soil amendment: a review of the environmental implications. Swansea University. School of the Environment and Society

  71. Zackrisson O, Nilsson M-C, Wardle DA (1996) Key ecological function of charcoal from wildfire in the boreal forest. Oikos:10–19

  72. Zhang QZ, Dijkstra FA, Liu XR, Wang YD, Huang J, Lu N (2014) Effects of biochar on soil microbial biomass after four years of consecutive application in the North China plain. PLoS One 9:e102062

  73. Zwart DC, Kim S-H (2012) Biochar amendment increases resistance to stem lesions caused by Phytophthora spp. in tree seedlings. HortScience 47:1736–1740

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Wagner Bettiol (CNPq 303899/2015-8) acknowledges Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the productivity fellowship.

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Silva, L.G., de Andrade, C.A. & Bettiol, W. Biochar amendment increases soil microbial biomass and plant growth and suppresses Fusarium wilt in tomato. Trop. plant pathol. (2020). https://doi.org/10.1007/s40858-020-00332-1

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  • Fusarium oxysporum f. sp. lycopersici
  • Solanum lycopersicum
  • Alternative control; microbial activity
  • Soil suppressiveness