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Tropical Plant Pathology

, Volume 44, Issue 2, pp 113–119 | Cite as

Biological control of Rhizoctonia solani in cowpea plants using yeast

  • Dyana A. de Tenório
  • Erika Valente de MedeirosEmail author
  • Cristiano S. Lima
  • Jéssica M. da Silva
  • Jamilly A. de Barros
  • Rejane P. Neves
  • Delson Laranjeira
Original Article
  • 62 Downloads

Abstract

Yeasts have been used to manage a large number of plant diseases, but little is known about the mechanisms used by these biocontrol agents. The objectives of the present study were to evaluate the antagonistic effect of yeasts against Rhizoctonia solani and possible mechanisms of action in cowpea plants. Seventy yeast isolates were obtained from leaf, root and stem tissues of cowpea and common bean plants. Screening experiments were conducted in a greenhouse at temperatures ranging from 15 to 26 °C in the first and from 22 to 31 °C in the second experiment. Candida saopaulonensis C6A, Cryptococcus laurentii FVC10 and Bullera sinensis FVF10 (R1) reduced disease severity by 57.4%, 48.5% and 66.3%, respectively. Cowpea plants treated with FVF10 (R1) showed the highest peroxidase and catalase activities. The mechanisms of action were based on competition and induction of enzymes such as peroxidase, catalase and ascorbate peroxidase in cowpea. Candida saopaulonensis C6A, C. laurentii FVC10 and B. sinensis FVF10 (R1) are potential biocontrol agents of damping-off and stem rot caused by R. solani on cowpea plants.

Keywords

Vigna unguiculata Antagonists Biocontrol Induction of plant resistance Soil-borne pathogens 

Notes

Acknowledgements

We thank CAPES for granting a scholarship to the first author, as well as CNPq for the research scholarship granted to DL, CSL, and EVM (306401/2015-0). We thank Dr. Antônio Félix da Costa (IPA) for his contribution concerning the plant material used in the present study.

Supplementary material

40858_2019_275_MOESM1_ESM.jpg (95 kb)
Supplementary Figure S1 The antagonist effect of yeast isolates against Rhizoctonia solani mycelial growth in vitro, calculated in relation to the control treatment without any yeast isolate. (JPG 95 kb)

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Copyright information

© Sociedade Brasileira de Fitopatologia 2019

Authors and Affiliations

  • Dyana A. de Tenório
    • 1
  • Erika Valente de Medeiros
    • 2
    Email author
  • Cristiano S. Lima
    • 3
  • Jéssica M. da Silva
    • 2
  • Jamilly A. de Barros
    • 2
  • Rejane P. Neves
    • 4
  • Delson Laranjeira
    • 1
  1. 1.Dep. de AgronomiaUniversidade Federal Rural de PernambucoRecifeBrazil
  2. 2.Universidade Federal Rural de PernambucoGaranhunsBrazil
  3. 3.Dep. de FitotecniaUniversidade Federal do CearáFortalezaBrazil
  4. 4.Dep. de MicologiaUniversidade Federal de PernambucoRecifeBrazil

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