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The population of Sclerotinia sclerotiorum affecting common bean in Brazil is structured by mycelial compatibility groups

  • Miller da S. Lehner
  • Rhaphael A. Silva
  • Trazilbo J. Paula Júnior
  • José Eustáquio S. Carneiro
  • Eduardo S. G. Mizubuti
ORIGINAL ARTICLE
  • 46 Downloads

Abstract

The genetic structure of a population of Sclerotinia sclerotiorum causing white mold on common bean in Brazil was studied using microsatellite (SSR) loci and mycelial compatibility groups (MCGs). A total of 300 isolates were analyzed and 154 SSR haplotypes and 32 MCGs were identified. Two MCGs were widely distributed and accounted for 70% of the isolates. Six SSR haplotypes were associated with more than one closely related MCG. There was no evidence of random association of alleles among loci when the population comprised by all MCGs was analyzed, suggesting that outcrossing is absent or rare. Nevertheless, there was evidence of random mating within the major MCGs. Seven genetic groups were identified, one of them comprising only highly pigmented isolates. Isolates of distinct MCGs did not differ in virulence. There was strong genetic differentiation among MCGs: more than 95% of the total genetic variation was attributed to differences among these groups. Therefore, MCGs contribute to the structure of the population of S. sclerotiorum in Brazil.

Keywords

White mold Genetics Variability Resistance Phaseolus vulgaris 

Notes

Acknowledgements

The authors were supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq. This research was supported by FAPEMIG and CNPq. The authors thank Dr. Antônio Félix da Costa, Dr. Hélcio Costa and Airton Luiz Pazinato for sampling of S. sclerotiorum isolates in Pernambuco, Espírito Santo and São Paulo state, respectively. The authors thank Dr. Linda Kohn for sending DNA of S. sclerotiorum isolate LMK211.

Supplementary material

40858_2018_270_MOESM1_ESM.docx (59 kb)
Supplementary table 1 Geographic origin and allele size at each microsatellite locus of 182 isolates of Sclerotinia sclerotiorum from common bean fields in Brazil. The data related to the remaining 118 isolates can be found at: https://onlinelibrary.wiley.com/doi/10.1111/ppa.12322 (DOCX 59 kb)
40858_2018_270_MOESM2_ESM.doc (36 kb)
Supplementary table 2 Number of alleles found in the Brazilian population of Sclerotinia sclerotiorum from common bean compared to those found in other studies, size range and gene diversity for each microsatellite locus. (DOC 36 kb)

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Copyright information

© Sociedade Brasileira de Fitopatologia 2018

Authors and Affiliations

  1. 1.Programa de Pós-graduação em Genética e MelhoramentoUniversidade Federal de ViçosaViçosaBrazil
  2. 2.Departamento de FitopatologiaUniversidade Federal de ViçosaViçosaBrazil
  3. 3.Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)ViçosaBrazil
  4. 4.Departamento de FitotecniaUniversidade Federal de ViçosaViçosaBrazil

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