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Movement of the bacterial blight pathogen Erwinia psidii in guava varieties differing in susceptibility

  • Dirceu Macagnan
  • Marisa A. S. V. Ferreira
Short Communication
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Abstract

Erwinia psidii causes bacterial blight of guava, an important disease of this fruit crop in Brazil. Pathogen spread is associated with the movement of infected but asymptomatic propagating plant material. The objective of this study was to compare bacterial movement inside the host tissue and symptom development in two guava varieties commonly grown in Brazil. Guava plants of Pedro Sato and Kumagai varieties were inoculated with strain IBSBF435 by deposition of a bacterial suspension droplet followed by puncture at the axils of the first fully expanded pair of leaves. Disease severity was assessed in the upper third part of the plant and stem fragments were cut at intervals of 1 cm above and below the inoculation point. Samples were collected at 0.5, 1, 2, 4, 6, 8, 10 and 12 days after inoculation. Presence of viable cells of E. psidii at each sampling point was evaluated using Bio-PCR with species-specific primers after a 24 h-enrichment step. Bacterial movement was detected on both directions in both varieties, however a positive correlation between movement and disease severity was observed only for Pedro Sato. Kumagai plants remained symptomless despite the presence of E. psidii above and below the inoculation site.

Keywords

Psidium guajava Bio-PCR Colonization 

Notes

Acknowledgements

The authors wish to thank Dr. Abi S. A. Marques for the helpful suggestions and CNPq for the fellowship to M. A. S. V. Ferreira.

Supplementary material

40858_2018_236_Fig3_ESM.png (926 kb)
Figure S1

Detection of Erwinia psidii in guava plants by Bio-PCR 48 h after inoculation. Agarose gel electrophoresis of PCR products amplified with primers Ep2L/Ep2R from tissue samples at various distances from the inoculation point. M, Molecular size marker 100-bp DNA ladder (Promega); C+, E. psidii cell suspension as a positive control; C-, water control. Numbers at each lane indicate the distance in cm (−, below, +, above) from the inoculation point. (PNG 926 kb)

40858_2018_236_MOESM1_ESM.tif (6.7 mb)
High resolution image (TIF 6899 kb)
40858_2018_236_MOESM2_ESM.jpg (547 kb)
Figure S2 Erwinia psidii movement in stem tissue of guava varieties Pedro Sato and Kumagai. The presence of bacteria in four plants of each variety at each distance (cm) from the inoculation point and at times between 12 h and 12 days after inoculation is indicated by red squares. (JPG 547 kb)
40858_2018_236_MOESM3_ESM.docx (14 kb)
Table S1 (DOCX 14 kb)

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Copyright information

© Sociedade Brasileira de Fitopatologia 2018

Authors and Affiliations

  1. 1.Instituto Federal de Educação, Ciência e Tecnologia de Brasília Campus PlanaltinaPlanaltinaBrazil
  2. 2.Departamento de FitopatologiaUniversidade de BrasíliaBrasíliaBrazil

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