Tropical Plant Pathology

, Volume 43, Issue 2, pp 170–177 | Cite as

Multi-host species of Cercospora are associated with Cercospora leaf blight and purple seed stain of soybean

  • L. L. Borges
  • T. F. Ferreira
  • M. G. Lana
  • I. D. Caliman
  • B. H. Bluhm
  • L. O. Oliveira
Short Communication

Abstract

Many Cercospora spp. are multi-host pathogens. The versatility of Cercospora newly associated with Cercospora leaf blight (CLB) and purple seed stain (PSS) in soybean seems to have been historically underestimated. Herein, we assembled multilocus sequence data (seven nuclear gene regions) with sequences from 36 fungal isolates sampled from non-soybean host species from Brazil (15 species) and United States (six species), in addition to 13 isolates from soybean. Phylogenetic tools explored relationships among isolates of Cercospora obtained from either soybeans or non-soybean hosts. Cercosporin production was determined to confirm whether the isolates belong to cercosporin-producing species of Cercospora. Among the five genealogical lineages, Lineage 1 showed C. kikuchii as the only member; this species is known to infect only soybean. The remaining four lineages were multi-host Cercospora which infected soybean along with ornamental and weed species. Genealogical placements were unrelated to host association and isolates clustered according to geography. Multi-host species of cercosporin-producing Cercospora are associated with CLB and PSS of soybean.

Keywords

Cercospora kikuchii DNA sequencing Fungal genetics Genetic diversity Glycine max Phylogeny Phylogeography 

Notes

Acknowledgements

We are grateful to those who provided assistance collecting samples. LLB received a scholarship from National Council of Scientific and Technological Development (CNPq; 140428/2014-3) and CAPES Foundation (8781/14-1). LOO received fellowships from CNPq (PQ 305827/2015-4, PDE 200786/2015-6) and grants from the Minas Gerais State Foundation of Research Aid (FAPEMIG; PPM 00561-15), CNPq (478752/2013–0), and CAPES (PPCP–Mercosul 015/2011). TFF received a fellowship from CAPES (PNPD 2010). MGL received a fellowship from CNPq (IC 104284/2016-1). IDC received a fellowship from CNPq (IC 121557/2014-6). BHB was supported by award #1253256 from the U.S. National Science Foundation. The National Institute of Food and Agriculture, U.S. Department of Agriculture (award #1008895 to BHB) provided additional support.

References

  1. Albu S, Schneider RW, Price PP, Doyle VP (2016) Cercospora Cf. flagellaris and C. Cf. sigesbeckiae are associated with Cercospora leaf blight and purple seed stain on soybean in North America. Phytopathology 106:1376–1385. CrossRefPubMedGoogle Scholar
  2. Alfenas AC, Ferreira FA, Mafia RG, Gonçalves RC (2016) Isolamento de fungos fitopatogênicos. In: Alfenas AC, Mafia RG (eds) Métodos em fitopatologia, 2nd edn. Viçosa, Brazil. Editora UFV, pp 55–91Google Scholar
  3. Almeida AMR, Piuga FF, Marin SRR, Binneck E, Sartori F, Costamilan LM, Teixeira MRO, Lopes M (2005) Pathogenicity, molecular characterization, and cercosporin content of Brazilian isolates of Cercospora kikuchii. Fitopatologia Brasileira 30:594–602. CrossRefGoogle Scholar
  4. Cai L, Giraud T, Zhang N, Begerow D, Cai G, Shivas RG (2011) The evolution of species concepts and species recognition criteria in plant pathogenic fungi. Fungal Diversity 50:121–133. CrossRefGoogle Scholar
  5. Chupp C (1954) A monograph of the fungus genus Cercospora. The Ronald Press, IthacaGoogle Scholar
  6. Cooperman CJ, Jenkins SF (1986) Conditions influencing growth and sporulation of Cercospora asparagi and Cercospora blight development in asparagus. Phytopathology 76:617–622.CrossRefGoogle Scholar
  7. Crous PW, Braun U (2003) Mycosphaerella and its anamorphs: 1. Names published in Cercospora and Passalora. Utrecht, The Netherlands: Centraalbureau voor Schimmelcultures (CBS)Google Scholar
  8. Crous PW, Groenewald JZ (2005) Hosts, species and genotypes: opinions versus data. Australasian Plant Pathology 34:463–470.CrossRefGoogle Scholar
  9. Daub ME, Herrero S, Chung KR (2005) Photoactivated perylenequinone toxins in fungal pathogenesis of plants. FEMS Microbiology Letters 252:197–206.CrossRefPubMedGoogle Scholar
  10. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
  11. Fajola AO (1978) Cercosporin, a phytotoxin from Cercospora spp. Physiology. Plant Pathology 13:157–164Google Scholar
  12. Fresenius G (1863) Beiträge zur Ökologie 3. Frankfurt, German: Heinrich Ludwig Brommer VerlagGoogle Scholar
  13. Goodwin SB, Dunkle LD, Zismann VL (2001) Phylogenetic analysis of Cercospora and Mycosphaerella based on the internal transcribed spacer region of ribosomal DNA. Phytopathology 91:648–658. CrossRefPubMedGoogle Scholar
  14. Groenewald M, Groenewald JZ, Braun U, Crous PW (2006) Host range of Cercospora apii and C. beticola and description of C. apiicola, a novel species from celery. Mycologia 98:275–285.CrossRefPubMedGoogle Scholar
  15. Groenewald JZ, Nakashima C, Nishikawa J, Shin HD, Park JH, Jama AN, Groenewald M, Braun U, Crous PW (2012) Species concepts in Cercospora: spotting the weeds among the roses. Studies in Mycology 75:115–170CrossRefPubMedCentralGoogle Scholar
  16. Jenns AE, Daub ME, Upchurch RG (1989) Regulation of cercosporin accumulation in culture by medium and temperature manipulation. Phytopathology 79:213–219.CrossRefGoogle Scholar
  17. Matsumoto T, Tomoyasu R (1925) Studies on the purple speck of soybean seed. Annals of the Phytopathological Society of Japan 1:1–14. CrossRefGoogle Scholar
  18. Paul PA, Munkvold GP (2005) Influence of temperature and relative humidity on sporulation of Cercospora zeae-maydis and expansion of gray leaf spot lesions on maize leaves. Plant Disease 89:624–630.CrossRefGoogle Scholar
  19. Pollack FG (1987) An annotated compilation of Cercospora names. Mycologia Memoir 12:1–212Google Scholar
  20. R Core Team (2017) R: a language and environment for statistical computing. R foundation for statistical computing. Available at: https://www.R-project.org/
  21. Satterthwaite FE (1946) An approximate distribution of estimates of variance components. Biometrics Bulletin 2:110–114.CrossRefPubMedGoogle Scholar
  22. Soares APG, Guillin EA, Borges LL, Silva ACT, Almeida AMR, Grijalba PE, Gottlieb AM, Bluhm BH, Oliveira LO (2015) More Cercospora species infect soybeans across the americas than meets the eye. PLoS One 10:e0133495.CrossRefPubMedPubMedCentralGoogle Scholar
  23. Upchurch RG, Ehrenshaft M, Walker DC, Sanders LA (1991) Genetic-transformation system for the fungal soybean pathogen Cercospora kikuchii. Applied Environmental Microbiology 57:2935–2939PubMedPubMedCentralGoogle Scholar
  24. Walters HJ (1980) Soybean leaf blight caused by Cercospora kikuchii. Plant Disease 64:961–962.CrossRefGoogle Scholar

Copyright information

© Sociedade Brasileira de Fitopatologia 2017

Authors and Affiliations

  • L. L. Borges
    • 1
  • T. F. Ferreira
    • 1
  • M. G. Lana
    • 1
  • I. D. Caliman
    • 1
  • B. H. Bluhm
    • 2
  • L. O. Oliveira
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyUniversidade Federal de ViçosaViçosaBrazil
  2. 2.University of ArkansasFayettevilleUSA

Personalised recommendations