Turnip leaf curl disease associated with two begomoviruses in south-eastern Iran
A handful of geminiviruses have been associated with turnip leaf curl disease. As part of our turnip surveys in Kerman province (south-eastern Iran), turnip samples showing mild leaf curl disease were collected and screened for turncurto- and begomoviruses by PCR. In four out of seven samples, we identified co-infection with begomoviruses of the species Tomato yellow leaf curl virus and Watermelon chlorotic stunt virus. Neither of these two begomovirus species have been described to be associated with turnip leaf curl disease in Iran. Using infectious clones and agroinoculation of turnip, tomato and watermelon seedlings, our results indicated that single and/or co-agroinoculation of plants with the two begomovirus constructs result in symptoms with different severity in turnip, tomato and watermelon.
KeywordsTomato yellow leaf curl virus Watermelon chlorotic stunt virus Antagonism Rolling circle amplification Synergism Turnip
This research work was supported by the Iran National Science Foundation (INSF) (Grant No. 92012168).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Alves-Júnior M, Alfenas-Zerbini P, Andrade EC, Esposito DA, Silva FN, da Cruz ACF, Ventrella MC, Otoni WC, Zerbini FM (2009) Synergism and negative interference during co-infection of tomato and Nicotiana benthamiana with two bipartite begomoviruses. Virology 387:257–266Google Scholar
- Brown JK, Zerbini FM, Navas-Castillo J, Moriones E, Ramos-Sobrinho R, Silva JCF, Fiallo-Olivé E, Briddon RW, Hernández-Zepeda C, Idris A, Malathi VG, Martin DP, Rivera-Bustamante R, Ueda S, Varsani A (2015) Revision of Begomovirus taxonomy based on pairwise sequence comparisons. Archives of Virology 160:1593–1619CrossRefPubMedGoogle Scholar
- Grimsley N, Hohn B, Hohn T, Walden R (1986) Agroinfection, an alternative route for viral-infection of plants by using the Ti plasmid. Proceedings of the National Academy of Sciences, USA 83:3282–3286Google Scholar
- Stewart C, Kon T, Rojas M, Graham A, Martin DP, Gilbertson R, Roye M (2014) Mixed infection of Sida jamaicensis in Jamaica reveals the presence of three recombinant begomovirus DNA-A components. Archives of Virology 159:2509–2512Google Scholar
- Tahir M, Amin I, Haider MS, Mansoor S, Briddon RW (2015) Ageratum enation virus, a begomovirus of weeds with the potential to infect crops. Viruses 7:647–665Google Scholar
- Varsani A, Roumagnac P, Fuchs M, Navas-Castillo J, Moriones E, Idris A, Briddon RW, Rivera-Bustamante R, Zerbini FM, Martin DP (2017) Capulavirus and Grablovirus: two new genera in the family Geminiviridae. Archives of Virology. 162:1819–1831Google Scholar