Influence of Vitamin D Status and Mechanical Loading on the Morphometric and Mechanical Properties of the Mouse Tibia
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It is well known that vitamin D and mechanical loading play important roles in bone growth and development. However, the combined effect of the maternal vitamin D status and mechanical loading on the bone quality of growing and mature bones is still unclear. The aim of this study was to investigate the influence of the antenatal vitamin D status and mechanical loading on bone morphometric and mechanical properties in juvenile and adult bones. C57BL/6J mice were used to generate vitamin D-replete and vitamin D-depleted dams. The left tibiae of 8-week-old and 16-week-old offspring were mechanically loaded in vivo for two weeks. Both tibiae were dissected and scanned using a μCT imaging system. It was found that in the bones of 10-week-old juvenile offspring, the antenatal vitamin D-replete group significantly increased trabecular bone volume fraction (Tb.BV/TV), trabecular thickness (Tb.Th), cortical thickness (Ct.Th), bone stiffness and failure load; significantly decreased trabecular separation (Tb.Sp) and cortical marrow area (Ct.MA) only in loaded tibiae; and markedly increased Tb.Sp and Ct.MA only in non-loaded tibiae. In the bones of the 18-week-old adult offspring, the antenatal vitamin D status had a minimal effect on the bone morphometric and mechanical parameters. These data imply that antenatal vitamin D repletion results in increased responses to mechanical loading only in the juvenile state, emphasizing the importance of a sufficient vitamin D supply during pregnancy and sufficient physical activities during the juvenile period to increase bone quality.
KeywordsVitamin D Mouse tibia Morphometric analysis Mechanical properties Finite element analysis
This work was funded by the National Natural Science Foundation of China (11702057, 11772086), the Chinese Fundamental Research Funds for the Central Universities (DUT18LK19) and the Open Fund from the State Key Laboratory of Structural Analysis for Industrial Equipment (GZ1611), Dalian University of Technology.
Compliance with Ethical Standards
Conflict of interest
The authors declare that there are no financial or personal relationships with other persons or organizations that might inappropriately influence this work.
- 3.Levchuk, A., Zwahlen, A., Weigt, C., Lambers, F. M., Badilatti, S. D., Schulte, F. A., et al. (2014). The Clinical Biomechanics Award 2012—presented by the European Society of Biomechanics: large scale simulations of trabecular bone adaptation to loading and treatment. Clin Biomech, 29(4), 355–362.CrossRefGoogle Scholar
- 11.De Souza, R. L., Matsuura, M., Eckstein, F., Rawlinson, S. C., Lanyon, L. E., & Pitsillides, A. A. (2005). Non-invasive axial loading of mouse tibiae increases cortical bone formation and modifies trabecular organization: a new model to study cortical and cancellous compartments in a single loaded element. Bone, 37(6), 810–818.CrossRefGoogle Scholar
- 12.Willie, B. M., Birkhold, A. I., Razi, H., Thiele, T., Aido, M., Kruck, B., et al. (2013). Diminished response to in vivo mechanical loading in trabecular and not cortical bone in adulthood of female C57BL/6 mice coincides with a reduction in deformation to load. Bone, 55(2), 335–346.CrossRefGoogle Scholar
- 20.Chen, Y., Dall Ara, E., Sales, E., Manda, K., Wallace, R., Pankaj, P., et al. (2017). Micro-CT based finite element models of cancellous bone predict accurately displacement once the boundary condition is well replicated: a validation study. J Mech Behav Biomed Mater, 65, 644–651.CrossRefGoogle Scholar
- 22.Easley, S. K., Jekir, M. G., Burghardt, A. J., Li, M., & Keaveny, T. M. (2010). Contribution of the intra-specimen variations in tissue mineralization to PTH- and raloxifene-induced changes in stiffness of rat vertebrae. Bone, 46(4), 1162–1169. https://doi.org/10.1016/j.bone.2009.12.009.CrossRefGoogle Scholar
- 25.Pistoia, W., van Rietbergen, B., Lochmüller, E. M., Lill, C. A., Eckstein, F., & Rüegsegger, P. (2002). Estimation of distal radius failure load with micro-finite element analysis models based on three-dimensional peripheral quantitative computed tomography images. Bone, 30(6), 842–848.CrossRefGoogle Scholar