Effect of Collagen-Polycaprolactone Nanofibers Matrix Coating on the In Vitro Cytocompatibility and In Vivo Bone Responses of Titanium
- 25 Downloads
The objective of this study was to improve the biomechanical performance of titanium (Ti) using a biocompatible electrospun nanofiber matrix. The study is based on the hypothesis that coating a Ti surface with a nanofiber matrix (NFM) made of collagen (CG) and polycaprolactone (PCL) electrospun nanofibers could increase the mechanical fixation of Ti/bone by improving the surface and cytocompatibility properties of Ti. This study prepared Ti samples with and without CG-PCL NFM coatings. This study determined the in vitro effects of each group of Ti samples on the surface topography and cytocompatibility (osteoblast cell adhesion, proliferation, mineralization and protein adsorption) properties. This study also determined in vivo interface shear strength and bone volume fraction of each group of Ti samples with bone using a rabbit model. This study found that the CG-PCL NFM coating on Ti improved the surface roughness, osteoblast cell adhesion, proliferation, mineralization and protein adsorption properties of Ti. In vivo studies found that interface shear strength of CG-PCL NFM-coated Ti/bone samples was significantly higher compared to those values of control Ti/bone samples (p value < 0.05) due to an increase in the amount of growth of the connective tissue joining the Ti implant. Therefore, the developed CG-PCL NFM coating technique should further be investigated for its potential in clinical applications.
KeywordsTitanium Bone Interface shear strength In vivo Osseointegration Cytocompatibility Implant
This publication was made possible by Grant Number 5P20GM103447 from the NIH and on-campus faculty Grant and CURE-STEM awards from the College of Math and Science and the University of Central Oklahoma Office of Research and Grants. The AON-1 osteonectin antibody developed by John D. Termine was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242. Special acknowledgment to Andria Hedrick, Research Imaging Facility at the College of Pharmacy in the University of Oklahoma Health Science Center, for µCT analysis support.
Compliance with Ethical Standards
Conflict of interest
The authors have no conflict of interest.
- 1.Wu, S., Liu, X., Yeung, K. W. K., Guo, H., Li, P., Hu, T., et al. (2013). Surface nano-architectures and their effects on the mechanical properties and corrosion behavior of Ti-based orthopedic implants. Surface & Coatings Technology, 233, 13–26. doi: 10.1016/j.surfcoat.2012.10.023.CrossRefGoogle Scholar
- 3.Pizzo, A., Kokini, K., Vaughn, L., Waisner, B., & Voytik-Harbin, S. (2005). Extracellular matrix (ECM) microstructural composition regulates local cell-ECM biomechanics and fundamental fibroblast behavior: a multidimensional perspective. Journal of Applied Physiology, 98(5), 1909–1921.CrossRefGoogle Scholar
- 5.Keun Kwon, I., Kidoaki, S., & Matsuda, T. (2005). Electrospun nano- to microfiber fabrics made of biodegradable copolyesters: Structural characteristics, mechanical properties and cell adhesion potential. Biomaterials, 26(18), 3929–3939. doi: 10.1016/j.biomaterials.2004.10.007.CrossRefGoogle Scholar
- 9.Khandaker, M., & Riahinezhad, S. (2016. US patent application no. 62312041.). Method and apparatus to coat a metal implant with an extracellular matrix made with electrospun fiber.Google Scholar
- 10.Collagen Protein (2016). Retrieved September 7, 2016.Google Scholar
- 12.Stadlinger, B., Pilling, E., Mai, R., Bierbaum, S., Berhardt, R., Scharnweber, D., et al. (2008). Effect of biological implant surface coatings on bone formation, applying collagen, proteoglycans, glycosaminoglycans and growth factors. Journal of Materials Science, 19(3), 1043–1049. doi: 10.1007/s10856-007-3077-7.Google Scholar
- 16.Okuno, O. (1989). Application of beta titanium alloys in dentistry. Dentistry in Japan, 26(1989), 101–104.Google Scholar
- 19.Peter, B., Gauthier, O., Laib, S., Bujoli, B., Guicheux, J., Janvier, P., et al. (2006). Local delivery of bisphosphonate from coated orthopedic implants increases implants mechanical stability in osteoporotic rats. Journal of Biomedical Materials Research Part A, 76(1), 133–143. doi: 10.1002/jbm.a.30456.CrossRefGoogle Scholar
- 21.Bhehler. (2012). Application guide polishing: Solutions for materials preparation, testing and analysis.Google Scholar
- 22.Whiteside, P., Matykina, E., Gough, J. E., Skeldon, P., & Thompson, G. E. (2010). In vitro evaluation of cell proliferation and collagen synthesis on titanium following plasma electrolytic oxidation. Journal of Biomedical Materials Research Part A, 94(1), 38–46. doi: 10.1002/jbm.a.32664.CrossRefGoogle Scholar
- 23.Click-iT® EdU Imaging Kits. (2011). https://tools.thermofisher.com/content/sfs/manuals/mp10338.pdf.
- 24.Currey, J. D. (2002). Bones: Structure and mechanics. Princeton: Princeton University Press.Google Scholar
- 25.Clark, R. A., Nielsen, L. D., Welch, M. P., & McPherson, J. M. (1995). Collagen matrices attenuate the collagen-synthetic response of cultured fibroblasts to TGF-beta. Journal of Cell Science, 108(Pt 3), 1251–1261.Google Scholar
- 30.Jinno, T., Goldberg, V. M., Davy, D., & Stevenson, S. (1998). Osseointegration of surface-blasted implants made of titanium alloy and cobalt-chromium alloy in a rabbit intramedullary model. Journal of Biomedical Materials Research, 42(1), 20–29. doi: 10.1002/(SICI)1097-4636(199810)42:1<20:AID-JBM4>3.0.CO;2-Q.CrossRefGoogle Scholar
- 31.Albu, M. G., Titorencu, I., & Ghica, M. V. (2011). Collagen-based drug delivery systems for tissue engineering, biomaterials. In R. Pignatello (Ed.), Applications for Nanomedicine. InTech: Rijeka.Google Scholar
- 34.Khandaker, M., Cody, J., Sultana, F., Barnes, M., Odejimi, T., & Vaughan, M. Evaluation of osseointegration of titanium and bone scaffold interfaces. In 2015 SEM Annual Conference & Exposition on Experimental and Applied Mechanics, Costa Mesa, CA USA, 2015. New York: Springer.Google Scholar