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Membraneless reproducible MoS2 field-effect transistor biosensor for high sensitive and selective detection of FGF21

  • Xinxing Gong (龚新星)
  • Yeru Liu (刘业茹)
  • Haiyan Xiang (向海燕)
  • Hang Liu (刘航)
  • Zhigang Liu (刘志刚)
  • Xiaorui Zhao (赵晓蕊)
  • Jishan Li (李继山)
  • Huimin Li (李惠敏)
  • Guo Hong (洪果)
  • Travis Shihao Hu
  • Hong Chen (陈洪)
  • Song Liu (刘松)Email author
  • Gang Yu (余刚)Email author
Articles
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Abstract

Fibroblast growth factor 21 (FGF21) serves as an essential biomarker for early detection and diagnosis of nonalcoholic fatty liver disease (NAFLD). It has received a great deal of attention recently in efforts to develop an accurate and effective method for detecting low levels of FGF21 in complex biological settings. Herein, we demonstrate a label-free, simple and high-sensitive field-effect transistor (FET) biosensor for FGF21 detection in a nonaqueous environment, directly utilizing two-dimensional molybdenum disulfide (MoS2) without additional absorption layers. By immobilizing anti-FGF21 on MoS2 surface, this biosensor can achieve the detection of trace FGF21 at less than 10 fg mL−1. High consistency and satisfactory reproducibility were demonstrated through multiple sets of parallel experiments for the MoS2 FETs. Furthermore, the biosensor has great sensitivity to detect the target FGF21 in complex serum samples, which demonstrates its great potential application in disease diagnosis of NAFLD. Overall, this study shows that thin-layered transition-metal dichalcogenides (TMDs) can be used as a potential alternative platform for developing novel electrical biosensors with high sensitivity and selectivity.

Keywords

FGF21 MoS2 FET biosensor disease diagnostics 

无膜且具有重现性的MoS2场效应晶体管生物传感 器用于高灵敏度和高选择性地检测FGF21

摘要

成纤维细胞生长因子21(FGF21) 是一种用于早期检测和诊断 非酒精性脂肪肝病(NAFLD)的必需生物标志物.最近,开发在复杂 生物环境中准确有效地检测血液中低浓度FGF21的方法受到了极 大的关注.本文展示了一种无标记、简单和高灵敏度的场效应晶 体管 (FET)生物传感器,用于在非水环境中检测FGF21. 通过对二硫 化钼(MoS2)表面进行功能化将抗FGF21牢固地固定在MoS2材料上, 使该生物传感器实现检测FGF21的检测限小于10 fg mL−1.多组平 行实验证明了MoS2 FET 的高度一致性和令人满意的再现性.此外, 生物传感器对复杂血清样品中的目标FGF21具有很高的敏感度,这 表明其在NAFLD疾病诊断中具有巨大的潜在应用前景.

Notes

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (21705036, 21475036, 51271074, 21476066, and 81572500), the Natural Science Foundation of Hunan Province, China (2018JJ3035), Hunan Young Talents (2016RS3036) and the Fundamental Research Funds for the Central Universities from Hunan University. Prof. Guo Hong acknowledges the Start-up Research Grant (SRG2016-00092-IAPME), Multi-year Research Grant (MYRG2018-00079-IAPME) of the University of Macau, Science and Technology Development Fund (081/2017/A2, 0059/2018/A2, 009/2017/AMJ) and Macao SAR (FDCT).

Supplementary material

40843_2019_9444_MOESM1_ESM.pdf (549 kb)
Membraneless reproducible MoS2 field-effect transistor biosensor for high sensitive and selective detection of FGF21

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Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Xinxing Gong (龚新星)
    • 1
  • Yeru Liu (刘业茹)
    • 1
  • Haiyan Xiang (向海燕)
    • 1
  • Hang Liu (刘航)
    • 1
  • Zhigang Liu (刘志刚)
    • 2
  • Xiaorui Zhao (赵晓蕊)
    • 1
  • Jishan Li (李继山)
    • 1
  • Huimin Li (李惠敏)
    • 1
  • Guo Hong (洪果)
    • 3
  • Travis Shihao Hu
    • 4
  • Hong Chen (陈洪)
    • 5
  • Song Liu (刘松)
    • 1
    Email author
  • Gang Yu (余刚)
    • 1
    Email author
  1. 1.Institute of Chemical Biology and Nanomedicine (ICBN), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical EngineeringHunan UniversityChangshaChina
  2. 2.Department of Head and Neck Oncology, The Cancer Center of the Fifth Affiliated Hospital of Sun Yat-sen University, Phase I Clinical Trial Laboratory, The Fifth Affiliated HospitalSun Yat-sen UniversityZhuhaiChina
  3. 3.Institute of Applied Physics and Materials EngineeringUniversity of MacauTaipa, MacauChina
  4. 4.Department of Mechanical EngineeringCalifornia State UniversityLos AngelesUSA
  5. 5.School of Materials Science and Energy EngineeringFoshan UniversityFoshanChina

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