Advertisement

Theoretical and Experimental Plant Physiology

, Volume 31, Issue 4, pp 475–481 | Cite as

Callose accumulation in roots of soybean seedlings under water deficit

  • Natalia Carolina Moraes Ehrhardt-Brocardo
  • Cileide Maria Medeiros CoelhoEmail author
  • Clovis Arruda Souza
  • Vanderléia Mathias
Article
  • 38 Downloads

Abstract

Callose is cell wall constitutive component of some plant tissues and it is synthesized and deposited in response to stresses (abiotic and biotic). Considering physiological seed quality as an attribute that determines the rapid and uniform emergence of seedlings under a wide range of environmental conditions, this study verified the callose accumulation in seedling roots of soybean cultivars showing contrasting seed vigor under water deficit. Six soybean cultivars were used and the physiological seed quality was determined by germination and vigor tests. Water deficit simulation was performed by germination tests with polyethylene glycol solution at − 0.4 MPa, and the control (0.0 MPa; deionized water). 24 h after radicle protrusion, three samples of 13 root tips were prepared for callose determination. The cultivar ‘BMX Potência RR’ presented lower germination (87%) when compared to the other cultivars. Vigor results allowed the separation of cultivars into two vigor categories, high and low. The callose concentration deposited on soybean root tissues ranged from 0.0511 to 0.1244 μg per root tip. This allowed the observation of the contrast in callose accumulation between the cultivars during the water deficit, and this is responsive to the greater susceptibility of low vigor cultivars to stress in order to isolate plant tissue through the deposition of a physical barrier. Callose accumulation can be used as indicative of susceptibility to water stress in soybean cultivars.

Keywords

Glycine max β-Glucan Vigor Abiotic stress 

Notes

Acknowledgements

NCME-B acknowledges the Programa Nacional de Pós-Doutorado/Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (PNPD/Capes-Brazil) for a Post-Doc fellowship granted. CMMC and CAS acknowledge Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil, Universal e Produtividade), Capes/Proap, UDESC/FAPESC (2018/TR653), UDESC/PIC, UDESC/PROMOP for the financial support.

Author contributions

NCME-B and VM conducted the research project, prepared samples for biochemical investigations, made statistical analyses, and wrote the manuscript. CMMC contributed to the discussion and revised the manuscript. CAS guided the planning of the research project, contributed to the discussion and revised the manuscript. All authors read and approved the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest regarding the publication of this article.

References

  1. Bewley JD, Black M (1994) Seeds: physiology of development and germination. Plenum Press, New YorkCrossRefGoogle Scholar
  2. Bortolotto RP, Menezes NL, Garcia DC, Mattioni NM (2008) Comportamento de hidratação e qualidade fisiológica das sementes de arroz. Bragantia 67:991–996CrossRefGoogle Scholar
  3. Braga LF, Sousa MP, Braga JF, Sá ME (1999) Efeito da disponibilidade hídrica do substrato na qualidade fisiológica de sementes de feijão. Rev Bras Sementes 21:95–102CrossRefGoogle Scholar
  4. Brasil. Ministério da Agricultura, Pecuária e Abastecimento (2009) Regras para Análise de Sementes (RAS). MAPA/ACS, BrasíliaGoogle Scholar
  5. Chen XY, Kim JY (2009) Callose synthesis in higher plants. Plant Signal Behav 4:489–492CrossRefGoogle Scholar
  6. Costa CJ, Villela FA, Bertoncello MR (2008) Pré-hidratação de sementes de ervilha e sua interferência na avaliação do potencial fisiológico. Rev Bras Sementes 30:198–207CrossRefGoogle Scholar
  7. Delgado CML, Coelho CMM, Buba GP (2015) Mobilization of reserves and vigor of soybean seeds under desiccation with glufosinate ammonium. J Seed Sci 37:154–161CrossRefGoogle Scholar
  8. Dutra AS, Teófilo EM (2007) Envelhecimento acelerado para avaliar o vigor de sementes de feijão caupi. Rev Bras Sementes 29:193–197CrossRefGoogle Scholar
  9. Ehrhardt-Brocardo NCM, Coelho CMM (2016) Hydration patterns and physiologic quality of common bean seeds. Semina: Ciênc Agrár 37:1791–1800Google Scholar
  10. Finch-Savage WE, Bassel GW (2015) Seed vigour and crop establishment: extending performance beyond adaptation. J Exp Bot 67:567–591CrossRefGoogle Scholar
  11. Gommers CMM, Monte E (2018) Seedling establishment: a dimmer switch-regulated process between dark and light signaling. Plant Physiol 176:1061–1074CrossRefGoogle Scholar
  12. Han C, Yin X, He D, Yang P (2013) Analysis of proteome profile in germinating soybean seed, and its comparison with rice showing the styles of reserves mobilization in different crops. PLoS ONE 8:e56947CrossRefGoogle Scholar
  13. Han Z, Bin W, Zhang J, Guo S, Zhang H, Xu L, Chen Y (2018) Mapping of QTLs associated with seed vigor to artificial aging using two ril populations in maize (Zea mays L.). Agric Sci 9:397–415Google Scholar
  14. Henning FA, Mertz LM, Jacob Junior EA, Machado RD, Fiss G, Zimmer PD (2010) Composição química e mobilização de reservas em sementes de soja de alto e baixo vigor. Bragantia 69:727–734CrossRefGoogle Scholar
  15. Jin N, Liu SM, Peng H, Huang WK, Kong LA, Wu YH, Chen YP, Ge FY, Jian H, Peng DL (2019) Isolation and characterization of Aspergillus niger NBC001 underlying suppression against Heterodera glycines. Sci Rep 9:1–13CrossRefGoogle Scholar
  16. Kavan HC, Catão HCRM, Caixeta F, Rocha CS, Castilho IM (2019) Accelerated aging periods and its effects on electric conductivity of popcorn seeds. Revista de Ciências Agrárias 42:40–48Google Scholar
  17. Lechowska K, Kubala S, Wojtyla Ł, Nowaczyk G, Quinet M, Lutts S, Garnczarska M (2019) New insight on water status in germinating Brassica napus seeds in relation to priming-improved germination. Int J Mol Sci 20:E540CrossRefGoogle Scholar
  18. Marcos-Filho J (2015a) Seed vigor testing: an overview of the past, present and future perspective. Sci Agric 72:363–374CrossRefGoogle Scholar
  19. Marcos-Filho J (2015b) Fisiologia de sementes de plantas cultivadas. ABRATES, LondrinaGoogle Scholar
  20. Marcos-Filho J, Kikuti ALP, Lima LB (2009) Procedures for evaluation of soybean seed vigor, including an automated computer imaging system. Rev Bras Sementes 31:102–112CrossRefGoogle Scholar
  21. Mohammadi PP, Moieni A, Hiraga S, Komatsu S (2012) Organ-specific proteomic analysis of drought-stressed soybean seedlings. J Proteomics 75:1906–1923CrossRefGoogle Scholar
  22. Nedukha OM (2015) Callose: localization, functions, and synthesis in plant cells. Cytol Genet 49:49–57CrossRefGoogle Scholar
  23. Prazeres CS, Coelho CMM (2017) Hydration curve and physiological quality of maize seeds subjected to water deficit. Semina: Ciênc Agrár 38:1179–1186Google Scholar
  24. Rajjou L, Duval M, Gallardo K, Catusse J, Bally J, Job C, Job D (2012) Seed germination and vigor. Annu Rev Plant Biol 63:507–533CrossRefGoogle Scholar
  25. Sivaguru M, Fujiwara T, Samaj J, Baluska F, Yang Z, Osawa H, Maeda T, Mori T, Volkmann D, Matsumoto H (2000) Aluminum-induced 1 → 3-beta-d-glucan inhibits cell-to-cell trafficking of molecules through plasmodesmata. A new mechanism of aluminum toxicity in plants. Plant Physiol 124:991–1006CrossRefGoogle Scholar
  26. Stass A, Horst WJ (2009) Callose in abiotic stress. In: Bacic A, Fincher GB, Stone BA (eds) Chemistry, biochemistry, and biology of 1-3 beta glucans and related polysaccharides. Academic Press, San Diego, pp 499–524CrossRefGoogle Scholar
  27. R Core Team (2019) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/
  28. Tunes LM, Pedroso DC, Badinelli PG, Tavares LC, Rufino CA, Barros ACSA, Muniz MFB (2011) Envelhecimento acelerado em sementes de azevém com e sem solução salina e saturada. Cienc Rural 41:33–37CrossRefGoogle Scholar
  29. Villela FA, Doni Filho L, Sequeira EL (1991) Tabela de potencial osmótico em função da concentração de polietileno glicol 6.000 e da temperatura. Pesqui Agropecu Bras 26:1957–1968Google Scholar
  30. Wissemeier AH, Diening A, Hergenröder A, Horst WJ, Mix-Wagner G (1992) Callose formation as parameter for assessing genotypical plant tolerance of aluminium and manganese. Plant Soil 146:67–75CrossRefGoogle Scholar
  31. Wolny E, Betekhtin A, Rojek M, Braszewska-Zalewska A, Lusinska J, Hasterok R (2018) Germination and the early stages of seedling development in Brachypodium distachyon. Int J Mol Sci 19:E2916CrossRefGoogle Scholar
  32. Yao LM, Zhong YP, Wang B, Yan JH, Wu TL (2019) BABA application improves soybean resistance to aphid through activation of phenylpropanoid metabolism and callose deposition. Pest Manag Sci.  https://doi.org/10.1002/ps.5526 CrossRefPubMedGoogle Scholar
  33. Zhang G, Hoddinott J, Taylor GJ (1994) Characterization of 1,3-β-d-glucan (callose) synthesis in roots of Triticum aestivum in response to aluminum toxicity. J Plant Physiol 144:229–234CrossRefGoogle Scholar

Copyright information

© Brazilian Society of Plant Physiology 2019

Authors and Affiliations

  1. 1.Departamento de AgronomiaUniversidade do Estado de Santa CatarinaLagesBrazil

Personalised recommendations