Theoretical and Experimental Plant Physiology

, Volume 31, Issue 1, pp 215–226 | Cite as

Insights into the spatial and temporal organisation of plant metabolism from network flux analysis

  • Thiago Batista Moreira
  • Janderson Moraes Lima
  • Guilherme Carvalho Coca
  • Thomas Christopher Rhys WilliamsEmail author


The great complexity of plant metabolism, with multiple subcellular compartments, cell types, tissues and organs, greatly complicates its analysis. This problem is compounded by the extensive changes that occur in metabolism over time, both during day/night cycles and throughout the course of an entire life cycle. In this context in silico network flux analysis, including both isotope labelling and stoichiometric modelling approaches, represent an important tool to study plant metabolism, providing a framework for the integration of data from a number of experimental strategies. Together, these methods have provided insight into the subcellular distribution of metabolic flux, the interactions between different cell types, source and sink relationships between organs, and how metabolic flux alters over time both at the cellular and whole plant scale. In this review we discuss how network flux analysis has contributed to our understanding of the spatial and temporal organisation of plant metabolism, looking in detail at key studies that address questions surrounding plant metabolism at different scales, from subcellular to whole plant.


Metabolic flux analysis Flux balance analysis Compartmentation Metabolic modelling Spatiotemporal organisation 



This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. The authors would also like to thank the Fundação de Apoio a Pesquisa do Distrito Federal (FAPDF) for support in the form of a doctoral grant for TBM and research funding for TCRW (Processo 193.000.193/2014).


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© Brazilian Society of Plant Physiology 2018

Authors and Affiliations

  1. 1.Department of Botany, Institute of Biological SciencesUniversity of BrasíliaBrasíliaBrazil

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