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ABCF3 regulates the expression of aquaporin genes and endoplasmic reticulum stress-related genes in Arabidopsis

  • Shuang Li
  • Dong Li
  • Ping Zhang
  • Ruling Wang
  • Liangliang Sun
  • Jinpeng Wan
  • Jin Xu
Article
  • 7 Downloads

Abstract

The ATP-binding cassette (ABC) F/GCN (general control non-derepressible) subfamily is a group of soluble ABC proteins that has two nucleotide binding domains (NBDs) but no transmembrane domains (TMDs). Previous study has indicated that ABCF3, also called GCN20/SCORD5, is involved in stress-associated protein translation control and defense responses to bacterial infection. Here, we show that ABCF3 regulates H2O2 uptake and endoplasmic reticulum (ER) stress responses in Arabidopsis. ABCF3 expressed mainly in leaves, stems, root tips, seeds, and anther. Analysis of abcf3 mutants and transgenic overexpressors (OX) of ABCF3 indicates that ABCF3 regulates H2O2 uptake. qRT-PCR analysis indicated that ABCF3 affects the expression of several aquaporin genes that were involved in H2O2 uptake in plants. In addition, abcf3 mutants exhibited enhanced sensitivity to ER stress and amino acid deprivation when challenged with ER stress inducers tunicamycin and amino acid synthesis inhibitor chlorsulfuron compared to wild type. Overexpression of AtABCF3 increases, whereas, abcf3 mutants depress the expression of ER stress-related genes. Taken together, these results indicated that ABCF3 regulates stress response by modulating aquaporin gene expression and ER stress responses.

Keywords

ABCF3 Endoplasmic reticulum stress Hydrogen peroxide Aquaporin Arabidopsis thaliana 

Notes

Acknowledgements

The authors gratefully acknowledge the Central Laboratory of the Xishuangbanna Tropical Botanical Garden for providing us with research facilities. This work was supported by the National Key Research and Development Program of China (2016YFC0501901), China National Natural Sciences Foundation (31772383, 31272239), Qinghai innovation platform construction project (2017-ZJ-Y20), and Yunnan Province Foundation for academic leader (2014HB043).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

40626_2018_116_MOESM1_ESM.docx (19 kb)
Supplementary material 1 (DOCX 20 kb)

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Copyright information

© Brazilian Society of Plant Physiology 2018

Authors and Affiliations

  • Shuang Li
    • 1
    • 2
  • Dong Li
    • 1
  • Ping Zhang
    • 1
    • 2
  • Ruling Wang
    • 1
  • Liangliang Sun
    • 1
  • Jinpeng Wan
    • 1
    • 2
  • Jin Xu
    • 1
  1. 1.CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglaChina
  2. 2.University of Chinese Academy of SciencesBeijingChina

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