Exogenous brassinosteroid application delays senescence and promotes hyponasty in Carica papaya L. leaves
- 176 Downloads
The application of brassinosteroids (BR) is associated with increased tolerance to various stresses such as those induced by pathogens, temperature, salinity and drought. Here, we studied the influence of BRs analogues on the leaf senescence of papaya juvenile plants. The objective was to determine whether BRs affect plant growth and leaf senescence by promoting or inhibiting the degradation of chlorophyll and/or leaf abscission. Two-month-old plants were divided into five treatments: C (control without BR application); BRWC (BR applied in the whole canopy); BRL1 (BR applied in the youngest fully expanded leaf, L1); BRL2 (BR applied in L2 which is the secondly more mature leaf in relation to L1) and BRL3 (BR applied in L3 which is the third more mature leaf in relation to L2). We concluded that BRs do not interfere with the senescence of the youngest leaf (L1) but in older leaves (L3) BRWC maintains the leaves greener and attached to the plant for a longer time when compared to the leaves from the other treatments. BR application in whole-canopy increased the plant height, and it caused a delay in leaf senescence and, consequently, in leaf abscission. In addition, hyponastic leaves developed after BR-application in the whole-canopy. The senescence process and leaf abscission in papaya, stimulated by exogenous BRs, were directly related to leaf age.
KeywordsChlorophylls Leaf abscission Leaf greenness Leaf ontogeny Papaya
BIOBRAS16 was kindly provided by researchers from INCA (Instituto Nacional de Ciencias Agrarias, La Habana, Cuba).
- Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D et al (2011) High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation. Plant Cell 23:873–894CrossRefPubMedPubMedCentralGoogle Scholar
- Gomes MMA, Netto AT, Campostrini E, Bressan-Smith R, Zullo MAT, Ferraz TM, Siqueira LN, Leal NR, Núñez-Vázquez M (2013) Brassinosteroid analogue affects the senescence in two papaya genotypes submitted to drought stress. Theor Exp Plant Phys 25:186–195Google Scholar
- He YJ, Xu RJ, Zhao YJ (1996) Enhancement of senescence by epibrassinolide in leaves of mung bean seedlings. Acta Phytophysiol Sin 22:58–62Google Scholar
- Jiménez VM, Mora-Newcomer E, Gutiérrez-Souto MV (2014) Biology of the papaya plant. In: Ming R, Moore PH (eds) Plant genetics and genomics: crops and models. Springer, New York, pp 17–33Google Scholar
- Lima RSN, Figueiredo FAMMA, Martins AO, Deus BCS, Ferraz TM, Gomes MMA, Sousa EF, Glenn DM, Campostrini E (2015) Partial rootzone drying (PRD) and regulated deficit irrigation (RDI) effects on stomatal conductance, growth, photosynthetic capacity, and water-use efficiency of papaya. Sci Hortic 183:13–22CrossRefGoogle Scholar
- Polko JK, van Zanten M, van Rooij JA, Marée AFM, Voesenek LACJ, Tarkowská D, Strnad M, Voesenek LACJ, Peeters AJM, Pierik R (2012) Ethylene-induced differential petiole growth in Arabidopsis thaliana involves local microtubule reorientation and cell expansion. New Phytol 193:339–348CrossRefPubMedGoogle Scholar
- Ribeiro Junior JI (2001) Análises estatísticas no SAEG. Universidade Federal de Viçosa, Viçosa, MGGoogle Scholar
- Yoshizawa E, Kaizuka M, Yamagami A, Takeuchi MH, Matsui M, Kakei Y, Shimada Y, Sakuta M, Osada H, Asami T, Nakano T (2014) BPG3 is a novel chloroplast protein that involves the greening of leaves and related to brassinosteroid signaling. Biosci Biotechnol Biochem 78:420–442CrossRefPubMedGoogle Scholar
- Zhiponova MK, Vanhoutte I, Boudolf V, Betti C, Dhondt S, Coppens F, Mille E, Maes S, González-García MP, Cano-Delgado AI, Inzé D, Beemster GTS, Veylder L, Russinova E (2013) Brassinosteroid production and signaling differentially control cell division and expansion in the leaf. New Phytol 197:490–502CrossRefPubMedGoogle Scholar