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Differences between muscle from osteoporotic and osteoarthritic subjects: in vitro study by diffusion-tensor MRI and histological findings

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Abstract

Background

Osteoarthritis and osteoporosis are strongly coupled with alterations of muscles quality and fats metabolism. However, there are no studies for investigating possible differences between osteoporotic and osteoarthritic muscles. Understanding muscle-bone and muscle-cartilage interactions would be of high clinical value.

Aim

Investigate potential microstructural and physiological differences between osteoporotic and osteoarthritic muscles by diffusion Nuclear Magnetic Resonance (NMR) imaging (diffusion MRI) and histological findings.

Methods

Vastus-lateralis muscles excised from osteoporotic (n = 26, T Score <  − 2.5, Kellgren–Lawrence ≤ 2) and osteoarthritic (n = 26, T Score >  − 2.5, Kellgren-–Lawrence 3 and 4) age-matched women were investigated by NMR relaxometry, diffusion-tensor imaging (DTI) at 9.4 T, and histological techniques. Intramyocellular (IMCL) and extramyocellular (EMCL) lipid were quantified. The percentage and mean diameters of fibers I and II were evaluated. Relationship between mean diffusivity (MD), fractional anisotropy (FA), the DTI eigenvalues (λ1, λ2, λ3), histological findings in muscles and clinical data (Kellgren–Lawrence and T score, age, menopausal age, body mass index) were studied. Pairwise comparisons between groups were made using one-way analysis of variance and correlation between variables was assessed with linear correlation analysis (Pearson’s r coefficient).

Results

Osteoporotic muscles showed higher MD, λ1,λ2, λ3 compared to osteoarthritis ones. This is explainable with a significant higher density of IMCL droplets found inside the osteoarthritic muscles and a large amount of fibrotic tissue and IMCL infiltration between fibers, i.e. in endomysium and perimysium that lead to a more hindered diffusion. Furthermore, histological analysis suggests mitochondrial degeneration as the origin of the greatest amount of IMCL droplets in osteoarthritic muscles.

Conclusion

This work highlights differences between muscles of osteoporotic and osteoarthritic subjects that can be quantified by NMR DTI investigations.

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Acknowledgements

This work was supported by Agenzia Spaziale Italiana (ASI) “Studio multidisciplinare degli effetti della microgravità sulle cellule ossee” (SMEMCO). Call for research n. DC-DTE-2011–033.

Author information

All authors meet all criteria for authorship. All authors had full access to all data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: GDP, UT, and SC. Acquisition of data: GDP, MS, MC, RI, abd EG. Analysis and interpretation of data: GDP, MS, RI, MC, EG, UT, and SC. Drafting of the manuscript. GDP and SC. Critical revision of the manuscript for important intellectual content: GDP, MS, RI, UT, and SC. Statistical analysis: GDP and SC. Obtained funding: UT. Administrative, technical and material support: MC and EG. Study supervision: SC. All authors have read and approved the final submitted manuscript.

Correspondence to Silvia Capuani.

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Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

All procedures performed were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The study was reviewed and approved by the independent ethics committee of Fondazione PTV Policlinico Tor Vergata, Rome, Italy.

Informed consent The present study complied with ethical standards and informed consent was obtained from all individual participants included in the study.

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Di Pietro, G., Scimeca, M., Iundusi, R. et al. Differences between muscle from osteoporotic and osteoarthritic subjects: in vitro study by diffusion-tensor MRI and histological findings. Aging Clin Exp Res (2020). https://doi.org/10.1007/s40520-020-01483-6

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Keywords

  • Osteoporosis
  • Osteoarthritis
  • Skeletal muscle
  • IMCL
  • DTI