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Gene expression profiling of hypoxic response in different models of senescent endothelial cells

  • Maria Patrizia Mongiardi
  • Matilde Merolle
  • Valentina Fustaino
  • Andrea Levi
  • Maria Laura FalchettiEmail author
Short Communication
  • 68 Downloads

Abstract

Endothelial cells senescence is a physiological process affecting vascular integrity. It can contribute to heart and arterial stiffening and remodeling, impaired angiogenesis, defective vascular repair, and with an increasing prevalence of atherosclerosis. Drugs used as antineoplastic therapies, targeting tumor as well as endothelial cells, can also trigger endothelial cells senescence. We demonstrated that a short pulse of axitinib, a specific inhibitor of vascular endothelial growth factor receptors, induces cell senescence of endothelial cells. Here, we performed a high-throughput gene expression analysis to characterize the response of proliferating versus senescent endothelial cells to hypoxia, the main trigger of neo-angiogenetic phenomena in tumors. We compared the response to hypoxia of replicative senescent cells, with that of axitinib or of DNA damage-induced senescence. Overall, we enlightened common and specific responses to different senescence inducers and changes in the Senescent Associated Secretory Phenotype.

Keywords

Senescence Endothelial cells Hypoxia RNAseq Axitinib Doxorubicin SASP 

Notes

Acknowledgements

This work was supported by the National Research Council of Italy (CNR), Research Project “Aging: molecular and technological innovations for improving the health of the elderly population” (Prot. MIUR 2867 25.11.2011) to AL and MLF.

Author contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Maria Patrizia Mongiardi, Matilde Merolle and Valentina Fustaino. The first draft of the manuscript was written by Maria Laura Falchetti and Andrea Levi. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Statement of human and animal rights

The experiments performed in the present manuscript do not envisage human subjects nor laboratory animals. The experiments were entirely carried on cell cultures.

Informed consent

For this type of study, formal consent is not required.

Supplementary material

40520_2019_1390_MOESM1_ESM.docx (35 kb)
Supplementary material 1 (DOCX 34 kb)
40520_2019_1390_MOESM2_ESM.txt (6.9 mb)
Supplementary material 2 (TXT 7044 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.CNR-Institute of Cell Biology and NeurobiologyMonterotondo Scalo, RomeItaly

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