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Adiponectin gene variants and abdominal obesity in an Iranian population

  • Moloud Payab
  • Mahsa M. AmoliEmail author
  • Mostafa Qorbani
  • Shirin Hasani-RanjbarEmail author
Original Article

Abstract

Introduction

Waist-to-height ratio (WHtR) can be effective for the diagnosis of abdominal obesity and the risk of type 2 diabetes. The role of genetic factors in the development of obesity has been broadly recognized. Adiponectin’s level is inversely correlated with body fat percentage and is reduced in obesity and type 2 diabetes. The aim of this study is to investigate the association between WHtR and adiponectin gene polymorphisms in Iranian population.

Methods

This study was conducted on 610 subjects from two Iranian populations. Anthropometric characteristics were measured by routine methods. Blood samples were collected in tubes (3–5 mL) containing EDTA and were stored at 20 °C. After DNA extraction, genotyping was performed using PCR–RFLP technique.

Results

There were statistically significant differences in genotype frequencies of −11391 G/A in centrally obese (WHtR >0.5) and noncentrally obese (WHtR ≤0.5) subjects (P value <0.044). In the former, the frequencies of GG and GA + AA genotypes were 89.4 and 10.6 %, respectively, while the frequencies of GG and GA + AA genotypes were 95.9 and 4.1 %, respectively, in noncentrally obese subjects.

Conclusions

The frequency of GG genotype was significantly increased in subjects with WHtR >0.5 compared to the other group. After adjustment for diabetes, abdominal obesity was significantly associated with the −11391 G/A polymorphism.

Keywords

Obesity Adiponectin Diabetes Abdominal obesity Polymorphism Waist-to-height ratio Metabolic syndrome 

Abbreviations

WHtR

Waist-to-height ratio

WC

Waist circumference

BMI

Body mass index

Notes

Acknowledgments

Implementation of this study was sponsored by Tehran University of Medical Sciences (Endocrinology and Metabolism Research Center).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical standard

The study has been approved by ethical committee of Tehran University of Medical Sciences.

Informed consent

Informed consents have been obtained from all patients participating in the study.

References

  1. 1.
    Jeffery RW, Sherwood NE (2008) Is the obesity epidemic exaggerated? No. Br Med J 336(7638):245. doi: 10.1136/bmj.39458.495127 CrossRefGoogle Scholar
  2. 2.
    Hajian-Tilaki KO, Heidari B (2007) Prevalence of obesity, central obesity and the associated factors in urban population aged 20–70 years, in the north of Iran: a population-based study and regression approach. Obes Rev 8(1):3–10. doi: 10.1111/j.1467-789X.2006.00235.x CrossRefPubMedGoogle Scholar
  3. 3.
    de Azeredo Passos VM, Barreto SM, Diniz LM, Lima-Costa MF (2005) Type 2 diabetes: prevalence and associated factors in Brazilian community—the Bambuí health and aging study. Sao Paulo Med J 123(2):66–71CrossRefGoogle Scholar
  4. 4.
    Lee J, Chen L, Snieder H et al (2010) Heritability of obesity related phenotypes and association with adiponectin gene polymorphisms in the chinese national twin registry. Ann Hum Genet 74(2):146–154. doi: 10.1111/j.1469-1809.2010.00565.x CrossRefPubMedGoogle Scholar
  5. 5.
    Schneider HJ, Klotsche J, Silber S, Stalla GK, Wittchen H-U (2011) Measuring abdominal obesity: effects of height on distribution of cardiometabolic risk factors risk using waist circumference and waist-to-height ratio. Diabetes Care 34(1):e7. doi: 10.2337/dc10-1794 CrossRefPubMedGoogle Scholar
  6. 6.
    Ashwell M, Lejeune S, McPherson K (1996) Ratio of waist circumference to height may be better indicator of need for weight management. Br Med J 312(7027):377. doi: 10.1136/312.7027.377 CrossRefGoogle Scholar
  7. 7.
    Lee JS, Aoki K, Kawakubo K, Gunji A (1995) A study on indices of body fat distribution for screening for obesity. J Occup Health 37(1):9–18Google Scholar
  8. 8.
    Tulloch-Reid MK, Williams DE, Looker HC, Hanson RL, Knowler WC (2003) Do measures of body fat distribution provide information on the risk of type 2 diabetes in addition to measures of general obesity? Comparison of anthropometric predictors of type 2 diabetes in Pima Indians. Diabetes Care 26(9):2556–2561. doi: 10.2337/diacare.26.9.2556 CrossRefPubMedGoogle Scholar
  9. 9.
    Lin W-Y, Lee L-T, Chen C-Y et al (2002) Optimal cut-off values for obesity: using simple anthropometric indices to predict cardiovascular risk factors in Taiwan. Int J Obes 26(9):1232–1238. doi: 10.1038/sj.ijo.0802040 CrossRefGoogle Scholar
  10. 10.
    Lorenzo C, Serrano-Ríos M, Martínez-Larrad MT et al (2007) Which obesity index best explains prevalence differences in type 2 diabetes mellitus? Obesity 15(5):1294–1301. doi: 10.1038/oby.2007.151 CrossRefPubMedGoogle Scholar
  11. 11.
    Mi SQ, Yin P, Hu N et al (2013) BMI, WC, WHtR, VFI and BFI: which indictor is the most efficient screening index on type 2 diabetes in Chinese community population. Biomed Environ Sci 26(6):485–491. doi: 10.3967/0895-3988.2013.06.009 CrossRefPubMedGoogle Scholar
  12. 12.
    Hadaegh F, Shafiee G, Azizi F (2009) Anthropometric predictors of incident type 2 diabetes mellitus in Iranian women. Ann Saudi Med 29(3):194–200. doi: 10.4103/0256-4947.51788 CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Hsieh SD, Muto T (2006) Metabolic syndrome in Japanese men and women with special reference to the anthropometric criteria for the assessment of obesity: proposal to use the waist to-height ratio. Prev Med 42(2):135–139. doi: 10.1016/j.ypmed.2005.08.007 CrossRefPubMedGoogle Scholar
  14. 14.
    Mehrab-Mohseni M, Tabatabaei-Malazy O, Hasani-Ranjbar S et al (2011) Endothelial nitric oxide synthase VNTR (intron 4 a/b) polymorphism association with type 2 diabetes and its chronic complications. Diabetes Res Clin Pract 91(3):348–352. doi: 10.1016/j.diabres.2010.12.030 CrossRefPubMedGoogle Scholar
  15. 15.
    Rankinen T, Zuberi A, Chagnon YC et al (2006) The human obesity gene map: the 2005 update. Obesity 14(4):529–644. doi: 10.1038/oby.2006.71 CrossRefPubMedGoogle Scholar
  16. 16.
    Bazzaz JT, Shojapoor M, Nazem H et al (2010) Methylenetetrahydrofolate reductase gene polymorphism in diabetes and obesity. Mol Biol Rep 37(1):105–109. doi: 10.1007/s11033-009-9545 CrossRefGoogle Scholar
  17. 17.
    Chandran M, Phillips SA, Ciaraldi T, Henry RR (2003) Adiponectin: more than just another fat cell hormone? Diabetes Care 26(8):2442–2450. doi: 10.2337/diacare.26.8.2442 CrossRefPubMedGoogle Scholar
  18. 18.
    Arita Y, Kihara S, Ouchi N et al (1999) Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity. Biochem Biophys Res Commun 257(1):79–83. doi: 10.1016/j.bbrc.2012.08.024 CrossRefGoogle Scholar
  19. 19.
    Hotta K, Funahashi T, Arita Y et al (2000) Plasma concentrations of a novel, adipose-specific protein, adiponectin, in type 2 diabetic patients. Arterioscler Thromb Vasc Biol 20(6):1595–1599. doi: 10.1161/01.ATV.20.6.1595 CrossRefGoogle Scholar
  20. 20.
    Weyer C, Funahashi T, Tanaka S et al (2001) Hypoadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia. J Clin Endocrinol Metab 86(5):1930–1935CrossRefGoogle Scholar
  21. 21.
    Ouchi N, Kihara S, Arita Y et al (1999) Novel modulator for endothelial adhesion molecules: adipocyte-derived plasma protein adiponectin. Circulation 100(25):2473–2476. doi: 10.1161/01.CIR.100.25.2473 CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Díez JJ, Iglesias P (2003) The role of the novel adipocyte-derived hormone adiponectin in human disease. Eur J Endocrinol 148(3):293–300CrossRefGoogle Scholar
  23. 23.
    Hanley AJG, Bowden D, Wagenknecht LE et al (2007) Associations of adiponectin with body fat distribution and insulin sensitivity in nondiabetic Hispanics and African-Americans. J Clin Endocrinol Metab 92(7):2665–2671. doi: 10.1210/jc.2006-2614 CrossRefPubMedGoogle Scholar
  24. 24.
    Hasani-Ranjbar S, Amoli MM, Tabatabaei-Malazy O et al (2012) Effect of adiponectin gene polymorphisms on waist circumference in patients with diabetes. J Diabetes Metab Disord 11(1):14. doi: 10.1186/2251-6581-11-14 CrossRefPubMedPubMedCentralGoogle Scholar
  25. 25.
    Yu Z, Han S, Cao X, Zhu C, Wang X, Guo X (2012) Genetic polymorphisms in adipokine genes and the risk of obesity: a systematic review and meta-analysis. Obesity 20(2):396–406. doi: 10.1038/oby.2011.148 CrossRefPubMedGoogle Scholar
  26. 26.
    Stumvoll M, Tschritter O, Fritsche A et al (2002) Association of the T-G polymorphism in adiponectin (Exon 2) with obesity and insulin sensitivity: interaction with family history of type 2 diabetes. Diabetes 51(1):37–41. doi: 10.2337/diabetes.51.1.37 CrossRefPubMedPubMedCentralGoogle Scholar
  27. 27.
    Bazzaz JT, Nazari M, Nazem H et al (2010) Apolipoprotein e gene polymorphism and total serum cholesterol level in Iranian population. J Postgrad Med 56(3):173–175. doi: 10.4103/0022-3859.68629 CrossRefPubMedGoogle Scholar
  28. 28.
    Genuth S, Alberti KGMM, Bennett P et al (2003) Follow-up report on the diagnosis of diabetes mellitus. Diabetes Care 26(11):3160–3167. doi: 10.2337/diacare.26.11.3160 CrossRefPubMedGoogle Scholar
  29. 29.
    Schäffler A, Büchler C, Müller-Ladner U et al (2004) Identification of variables influencing resistin serum levels in patients with type 1 and type 2 diabetes mellitus. Horm Metab Res 36(10):702–707. doi: 10.1055/s-2004-826015 CrossRefPubMedGoogle Scholar
  30. 30.
    Tabatabaei-Malazy O, Hasani-Ranjbar S, Amoli MM et al (2010) Gender-specific differences in the association of adiponectin gene polymorphisms with body mass index. Rev Diabetic Stud 7(3):241–246. doi: 10.1900/RDS.2010.7.241 CrossRefPubMedGoogle Scholar
  31. 31.
    Esmaillzadeh A, Mirmiran P, Azizi F (2006) Comparative evaluation of anthropometric measures to predict cardiovascular risk factors in Tehranian adult women. Public Health Nutr 9(1):61–69. doi: 10.1079/PHN2005833 CrossRefPubMedGoogle Scholar
  32. 32.
    Hadaegh F, Zabetian A, Harati H, Azizi F (2006) Waist/height ratio as a better predictor of type 2 diabetes compared to body mass index in Tehranian adult men—a 3.6-year prospective study. Exp Clin Endocrinol Diabetes 114(6):310–315. doi: 10.1055/s-2006-924123 CrossRefPubMedGoogle Scholar
  33. 33.
    Lear SA, Chen MM, Frohlich JJ, Birmingham CL (2002) The relationship between waist circumference and metabolic risk factors: cohorts of European and Chinese descent. Metab Clin Exp 51(11):1427–1432. doi: 10.1053/meta.2002.34042 CrossRefPubMedGoogle Scholar
  34. 34.
    Molarius A, Seidell JC (1998) Selection of anthropometric indicators for classification of abdominal fatness—a critical review. Int J Obes 22(8):719–727. doi: 10.1038/sj.ijo.0800660 CrossRefGoogle Scholar
  35. 35.
    Lee S, Bacha F, Gungor N, Arslanian SA (2006) Racial differences in adiponectin in youth: relationship to visceral fat and insulin sensitivity. Diabetes Care 29(1):51–56. doi: 10.2337/diacare.29.01.06.dc05-0952 CrossRefPubMedGoogle Scholar
  36. 36.
    Asayama K, Hayashibe H, Dobashi K et al (2003) Decrease in serum adiponectin level due to obesity and visceral fat accumulation in children. Obes Res 11(9):1072–1079CrossRefGoogle Scholar
  37. 37.
    Wu H-Y, Xu S-Y, Chen L-L, Zhang H-F (2009) Waist to height ratio as a predictor of abdominal fat distribution inmen. Chin J Physiol 52(6):441–445. doi: 10.4077/CJP.2009.AMH064 CrossRefPubMedGoogle Scholar
  38. 38.
    Lee S, Kuk JL, Hannon TS, Arslanian SA (2008) Race and gender differences in the relationships between anthropometrics and abdominal fat inyouth. Obesity 16(5):1066–1071. doi: 10.1038/oby.2008.13 CrossRefPubMedGoogle Scholar
  39. 39.
    Dolley G, Bertrais S, Frochot V et al (2008) Promoter adiponectin polymorphisms and waist/hip ratio variation in a prospective French adults study. Int J Obes 32(4):669–675. doi: 10.1038/sj.ijo.0803773 CrossRefGoogle Scholar
  40. 40.
    Fumeron F, Aubert R, Siddiq A et al (2004) Adiponectin gene polymorphisms and adiponectin levels are independently associated with the development of hyperglycemia during a 3-year period: the epidemiologic data on the insulin resistance syndrome prospective study. Diabetes 53(4):1150–1157. doi: 10.2337/diabetes.53.4.1150 CrossRefPubMedGoogle Scholar
  41. 41.
    Bouatia-Naji N, Meyre D, Lobbens S et al (2006) ACDC/adiponectin polymorphisms are associated with severe childhood and adult obesity. Diabetes 55(2):545–550. doi: 10.2337/diabetes.55.02.06 CrossRefPubMedGoogle Scholar
  42. 42.
    Tankó LB, Siddiq A, Lecoeur C et al (2005) ACDC/adiponectin and PPAR-gamma gene polymorphisms: implications for features of obesity. Obes Res 13(12):2113–2121CrossRefGoogle Scholar
  43. 43.
    Jang Y, Chae JS, Koh SJ et al (2008) The influence of the adiponectin gene on adiponectin concentrations and parameters of metabolic syndrome in non-diabetic Korean women. Clin Chim Acta 391(1–2):85–90. doi: 10.1016/j.cca.2008.02.011 CrossRefPubMedGoogle Scholar
  44. 44.
    Menzaghi C, Ercolino T, Paola RD et al (2002) A haplotype at the adiponectin locus is associated with obesity and other features of the insulin resistance syndrome. Diabetes 51(7):2306–2312. doi: 10.2337/diabetes.51.7.2306 CrossRefGoogle Scholar
  45. 45.
    Ranjith N, Pegoraro RJ, Shanmugam R (2011) Obesity associated genetic variants in young Asian Indians with the metabolic syndrome and myocardial infarction. Cardiovasc J Afr 22(1):25–30. doi: 10.5830/CVJA-2010-036 CrossRefPubMedPubMedCentralGoogle Scholar
  46. 46.
    Arnaiz-Villena A, Fernández-Honrado M, Rey D et al (2013) Amerindians show association to obesity with adiponectin gene SNP45 and SNP276: population genetics of a food intake control and ‘thrifty’ gene. Mol Biol Rep 40(2):1819–1826. doi: 10.1007/s11033-012-2236-1 CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Obesity and Eating Habits Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences InstituteTehran University of Medical Sciences, Shariati HospitalTehranIran
  2. 2.Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences InstituteTehran University of Medical SciencesTehranIran
  3. 3.Department of Community MedicineAlborz University of Medical SciencesKarajIran
  4. 4.Non-Communicable Diseases Research Center, Endocrinology and Metabolism Population Sciences InstituteTehran University of Medical SciencesTehranIran
  5. 5.Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences InstituteTehran University of Medical SciencesTehranIran

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