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Differential effect of salinity on thermotolerance of SOD isoforms in seven varieties of Vigna mungo (L.) Hepper

  • Khushwant Singh
  • Sanjay K. Yadav
  • Ranjit K. MishraEmail author
Original Article
  • 18 Downloads

Abstract

Seven varieties of Vigna mungo, collected from different geographical regions of India, were chosen for a comparative analysis of SOD isoenzyme profiles under salt stress, and to determine if prior exposure of the seedlings to salt stress resulted in any alteration in thermotolerance of the SOD isoforms. Three SOD isoforms Mn-SOD, Fe-SOD and Cu/Zn-SOD were present in both leaves and roots of all the varieties. Fe-SOD and Cu/Zn-SOD were most predominant in leaves whereas the roots predominantly had Fe-SOD. Mn-SOD was least abundant in leaves, while it was the second most abundant SOD isoform in roots. Salt-induced changes in SOD isoform profiles were not uniform either in leaves or roots. Cu/Zn-SOD was found to be more responsive to salinity than the other SOD isoforms in both tissues. Prior exposure of seedlings to salt stress resulted in increase in thermotolerance of Cu/Zn-SOD in leaves of IPU-2-43, Shekhar-2, Azad-2 and UH-1 and roots of IPU-2-43 and Shekhar-2 varieties. The UH-1 variety appeared unique as it possessed an additional Cu/Zn-SOD isoform in roots, which was induced by salt as well as heat. This variety also exhibited a new Fe-SOD band which was heat-induced in roots of seedlings that were not exposed to salt, but disappeared in roots which were salt stressed prior to heat exposure. Hence, the seven varieties exhibited diversity in behavior with respect to SOD activities under salinity and high temperature.

Keywords

Superoxide dismutase (SOD) SOD isoforms Vigna mungo Salt stress Thermotolerance 

Abbreviations

ROS

Reactive oxygen species

SOD

Superoxide dismutase

Mn-SOD

Manganese superoxide dismutase

Fe-SOD

Iron superoxide dismutase

Cu/Zn-SOD

Copper/zinc superoxide dismutase

Notes

Author’s contributions

Study concept and designing was done by KS and RKM. Experimental laboratory work done by KS and SY. Literature search by KS, SY and RKM. Data analysis by KS, SY and RKM. Manuscript preparation by KS, SY and RKM.

Funding

This study was financially supported by Department of Science and Technology-Promotion of University Research and Scientific Excellence (DST-PURSE) Grant to Lucknow University, and Council of Science and Technology Uttar Pradesh research Project No. CST/SERPD/D/1198 to RKM. KS acknowledges Junior Research Fellowship from University Grant Commission, New Delhi (Ref. No. 21/12/2014(ii)EU-V).

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Copyright information

© Indian Society for Plant Physiology 2019

Authors and Affiliations

  • Khushwant Singh
    • 1
  • Sanjay K. Yadav
    • 1
    • 2
  • Ranjit K. Mishra
    • 1
    Email author
  1. 1.Department of BiochemistryUniversity of LucknowLucknowIndia
  2. 2.Department of BiochemistrySam Higginbottom University of Agriculture, Technology and Sciences (SHUATS)AllahabadIndia

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