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Identification and optimization of ISSR primers for analysis of genetic variability among different landrace collections of Indian sponge gourd (Luffa cylindrica L.)

  • Ankur BhardwajEmail author
Original Article
  • 7 Downloads

Abstract

Luffa cylindrica L. or sponge gourd is an economically important horticultural crop from Indian origin and belongs to family Cucurbitaceae. It has extremely variable characteristics like fruit shape, size, quality etc. Though, the genetic variety and population structure in Luffa species is not well implicited neither previously reported accurately. Thus, present work aims to optimize methodology for identification of genetic diversity and population structure among its different varieties and landrace assortments using PCR based (ISSR) markers. The study will help us in designing investigation for large scale characterization of genetic variability across different L. cylindrica. germplasm plus gene pool which could benefit breeding programs. In the present work ISSR markers were used in genetic profiling of 58 sponge gourd accessions using five selected ISSR primers. The landrace collection which were giving maximum amplification were then phylogenetically analyzed to identify their closest relationship with other varieties of Sponge gourd. The three landrace (Luffa-886, Luffa-889 and Luffa-890) germplasm were carefully chosen based on their polymorphic results and phylogenetic relationship were established. This study also provides information for Luffa genotypes, as well as the polymorphic markers identified could be available for the study of landmarks in linkages, evolutionary history and marker-assisted selection in Luffa species.

Keywords

Tris–EDTA (T.E) Buffer Luffa cylindrica L. Sponge gourd ISSR Primers Genetic diversity PCR 

Abbreviations

C-TAB

Cetyl-tri methyl ammonium bromide

CIA

Chloroform iso-amyl alcohol

ISSR

Inter simple sequence repeat

PVP

Polyvinyl pyrrolidone

PCI

Phenol:chloroform:isoamylalcohol

PCR

Polymerase chain reaction

RPM

Revolution per minute

RT

Room temperature

SDS

Sodium dodecyl sulphate

TE

Tris EDTA

Notes

Acknowledgements

The authors are thankful to Dr. Dipnarayan Saha, Senior Scientist-Plant Biotechnology, NRC-DNA Fingerprinting, NBPGR (ICAR), Pusa campus, New Delhi and Dr. Gajendra B. Singh, Department of Biotechnology and Life Sciences, Mangalayatan University and for their suggestions in the preparation of research article.

Funding

The research is funded by National Bureau of Plant Genetic Resources (NBPGR), Pusa campus, New Delhi.

Compliance with ethical standards

Ethical approval

This review article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Abasaheb, R., Swati, S., Gajanan, L., Sambhaji, D., & Vaishali, V. (2015). Rapid biosynthesis of silver nanoparticles using bottle gourd fruit extract and potential application as bactericide. Research in Pharmacy, 3(3), 22–28.Google Scholar
  2. Bargali, K. (2015). Comparative participation of rural women in agroforestry home gardens in Kumaun Himalaya, Uttarakhand, India. Asian Journal of Agricultural Extension, Economics and Sociology, 6(1), 16–22.Google Scholar
  3. Browning, M. (1999). Natural soapmaking. New York: Sterling Publishing Company Inc.Google Scholar
  4. Choudhary, B., Kumar, S., & Sharma, S. (2014). Evaluation and correlation for growth, yield and quality traits of ridge gourd (Luffa acutangula) under arid conditions. Indian Journal of Agricultural Sciences, 84(4), 498–502.Google Scholar
  5. Daniel, P., & Yadav, K. (2016). Developing the insulation sheet of luffa cylindrica for mitticool fridge. International Journal of Scientific Research and Management, 4(8), 4514–4524.Google Scholar
  6. Gupta, A., Sood, S., Agrawal, P., & Bhatt, J. (2013). Under-utilized food crops of Himalayan region: Utilization and prospective (pp. 101–120). Delhi: Narendra Publishing House.Google Scholar
  7. Hu, J., Gao, L., Xu, Y., Li, Q., Zhu, H., Yang, L., et al. (2019). Microsatellite markers reveal genetic diversity and relationships within a melon collection mainly comprising asian cultivated and wild germplasms. BioMed Research International.  https://doi.org/10.1155/2019/7495609.Google Scholar
  8. Jamwal, M., & Sharma, N. (2015). Reproductive efficiency of two Luffa species-Factors affecting low reproductive rate in meiotically stable Luffa acutangula (L.) Roxb. The Nucleus, 58(1), 59–65.Google Scholar
  9. Khedkar, R., & Singh, K. (2018). Food industry waste: A panacea or pollution hazard? Paradigms in pollution prevention (pp. 35–47). Cham: Springer.Google Scholar
  10. Kumar, M., Pawar, S. V., Masurkar, P., & Choudhary, S. (2017). Molecular markers: A new frontier approaches in crop improvement. IJCS, 5(4), 1724–1729.Google Scholar
  11. Mariod, A. A., Mirghani, M. E. S., & Hussein, I. H. (2017). Unconventional oilseeds and oil sources. Cambridge: Academic Press.Google Scholar
  12. Misra, S., Srivastava, A. K., Verma, S., Pandey, S., Bargali, S. S., Rana, T. S., et al. (2017). Phenetic and genetic diversity in Indian Luffa (Cucurbitaceae) inferred from morphometric, ISSR and DAMD markers. Genetic Resources and Crop Evolution, 64(5), 995–1010.Google Scholar
  13. Nadeem, M. A., Nawaz, M. A., Shahid, M. Q., Doğan, Y., Comertpay, G., Yıldız, M., et al. (2017). DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing. Biotechnology and Biotechnological Equipment, 32, 1–25.Google Scholar
  14. Pandey, S., Ansari, W., Choudhary, B., Pandey, M., Jena, S., Singh, A., et al. (2018). Microsatellite analysis of genetic diversity and population structure of hermaphrodite ridge gourd (Luffa hermaphrodita). 3 Biotech, 8(1), 17.Google Scholar
  15. Rehman, A., & Adnan, M. (2018). Nutritional potential of Pakistani medicinal plants and their contribution to human health in times of climate change and food insecurity. Pakistan Journal of Botany, 50(1), 287–300.Google Scholar
  16. Reddy, M. P., Sarla, N., & Siddiq, E. A. (2002). Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, 128(1), 9–17.Google Scholar
  17. Satyanarayana, K. G., Flores-Sahagun, T. H., & Bowman, P. (2018). Lignocellulosic materials of Brazil—Their characterization and applications in polymer composites and art works. In S. Kalia (Ed.), Lignocellulosic Composite Materials (pp. 1–96). Cham: Springer.Google Scholar
  18. Semagn, K., Bjørnstad, Å., & Ndjiondjop, M. (2006). An overview of molecular marker methods for plants. African Journal of Biotechnology, 5(25), 2540–2568.Google Scholar
  19. Swamy, K., & Sadashiva, A. (2007). Tropical vegetable crops. Biodiversity in Horticultural Crops, 1, 109–183.Google Scholar
  20. Viswanathan, V., Krishnan, D., Kalra, S., Chawla, R., Tiwaskar, M., Saboo, B., et al. (2019). Insights on medical nutrition therapy for type 2 diabetes mellitus: An Indian perspective. Advances in Therapy, 36(3), 520–547.Google Scholar
  21. Vohora, S., Rizwan, M., & Khan, J. (1973). Medicinal uses of common Indian vegetables. Planta Medica, 23(04), 381–393.Google Scholar
  22. Wu, H., He, X., Gong, H., Luo, S., Li, M., Chen, J., et al. (2016). Genetic linkage map construction and QTL analysis of two interspecific reproductive isolation traits in sponge gourd. Frontiers in Plant Science, 7, 980.Google Scholar
  23. Xinyi, W., Pei, X., Xiaohua, W., Baogen, W., Zhongfu, L., & Guojing, L. (2017). Development of insertion and deletion markers for bottle gourd based on restriction site-associated DNA sequencing data. Horticultural Plant Journal, 3(1), 13–16.Google Scholar

Copyright information

© Indian Society for Plant Physiology 2019

Authors and Affiliations

  1. 1.Department of Biotechnology and Life Sciences, Institute of Biomedical Education and ResearchMangalayatan UniversityBeswan, AligarhIndia

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