Identification and optimization of ISSR primers for analysis of genetic variability among different landrace collections of Indian sponge gourd (Luffa cylindrica L.)

  • Ankur BhardwajEmail author
Original Article


Luffa cylindrica L. or sponge gourd is an economically important horticultural crop from Indian origin and belongs to family Cucurbitaceae. It has extremely variable characteristics like fruit shape, size, quality etc. Though, the genetic variety and population structure in Luffa species is not well implicited neither previously reported accurately. Thus, present work aims to optimize methodology for identification of genetic diversity and population structure among its different varieties and landrace assortments using PCR based (ISSR) markers. The study will help us in designing investigation for large scale characterization of genetic variability across different L. cylindrica. germplasm plus gene pool which could benefit breeding programs. In the present work ISSR markers were used in genetic profiling of 58 sponge gourd accessions using five selected ISSR primers. The landrace collection which were giving maximum amplification were then phylogenetically analyzed to identify their closest relationship with other varieties of Sponge gourd. The three landrace (Luffa-886, Luffa-889 and Luffa-890) germplasm were carefully chosen based on their polymorphic results and phylogenetic relationship were established. This study also provides information for Luffa genotypes, as well as the polymorphic markers identified could be available for the study of landmarks in linkages, evolutionary history and marker-assisted selection in Luffa species.


Tris–EDTA (T.E) Buffer Luffa cylindrica L. Sponge gourd ISSR Primers Genetic diversity PCR 



Cetyl-tri methyl ammonium bromide


Chloroform iso-amyl alcohol


Inter simple sequence repeat


Polyvinyl pyrrolidone




Polymerase chain reaction


Revolution per minute


Room temperature


Sodium dodecyl sulphate





The authors are thankful to Dr. Dipnarayan Saha, Senior Scientist-Plant Biotechnology, NRC-DNA Fingerprinting, NBPGR (ICAR), Pusa campus, New Delhi and Dr. Gajendra B. Singh, Department of Biotechnology and Life Sciences, Mangalayatan University and for their suggestions in the preparation of research article.


The research is funded by National Bureau of Plant Genetic Resources (NBPGR), Pusa campus, New Delhi.

Compliance with ethical standards

Ethical approval

This review article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Indian Society for Plant Physiology 2019

Authors and Affiliations

  1. 1.Department of Biotechnology and Life Sciences, Institute of Biomedical Education and ResearchMangalayatan UniversityBeswan, AligarhIndia

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