Comparison between various concentrations of commercial and synthesized silver nanoparticles on biochemical parameters and growth of Stevia rebaudiana B.

  • Moazzameh Ramezani
  • Mahyar GeramiEmail author
  • Zohreh Majlesi
Original Article


Nanoparticles are particles between 1 and 100 nm in size that has the ability to modify their physio-chemical properties compared to other material. In the present study, leaves of Stevia rebaudiana B. were used to extracts to synthesize Ag nanoparticles from AgNO3 (1 mM concentration) and then studied the effects of these commercial and synthesized Ag nanoparticles on biochemical (chlorophyll content, anthocyanin, flavonoid, carbohydrate, protein and DPPH) and growth characteristics of Stevia at different concentrations (0, 10, 20, 40 mM). UV–visible spectroscopy was used to identify the formation of Ag and analyzed synthesized Ag nanoparticles at 300–700 nm. Absorption maxima at 435 nm wavelength confirmed their synthesis. Examining synthesized AgNPs from S. rebaudiana extract using SEM micrograph showed a 25 nm dimension and spherical shape. Application of synthesized and commercial AgNPs at various concentrations on Stevia showed a dependency on concentrations that at 10 and 20 mM of AgNP, an increase in leaf area, shoot height and dry and fresh weight was recorded. At the biochemical level, applying 40 mM of synthesized AgNP resulted in increased chlorophyll content which led to accumulation of biomass besides soluble anthocyanin, flavonoid and carbohydrate and also total protein and DPPH, compared to plants treated with commercial AgNP. Moreover, the results demonstrated that increasing AgNP concentration enhances glycoside content in both treatments. Based on our findings, synthesized AgNP is more effective in accelerating the growth and improve the quality of natural product in Stevia plants than commercial AgNP.


Green synthesis silver nanoparticle Plant extracts Silver nanoparticle Stevia rebaudiana 



Authors would like to thank Sana Institute for providing the opportunity to conduct this research. Sari, Iran.

Authors’ contributions

MR: design the experiment, ZM: perform the experiment, MG and MR: analysis the data, MR and MG and ZM: interpret the data and final revision of the manuscript. MR and MG and ZM: approved the final revision of manuscript.


This paper was supported by Sana Institute, Iran.

Compliance with ethical standards

Consent for publication

Not applicable.

Supplementary material

40502_2018_413_MOESM1_ESM.docx (66 kb)
Supplementary material 1 (DOCX 66 kb)


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Copyright information

© Indian Society for Plant Physiology 2018

Authors and Affiliations

  • Moazzameh Ramezani
    • 1
  • Mahyar Gerami
    • 2
    Email author
  • Zohreh Majlesi
    • 3
  1. 1.Department of BiologyUrmia UniversityUrmiaIran
  2. 2.Faculty of Sana Institute of Higher EducationSariIran
  3. 3.Sana Institute of Higher EducationSariIran

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