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Indian Journal of Plant Physiology

, Volume 23, Issue 2, pp 369–375 | Cite as

Non-invasive quantification of tomato (Solanum lycopersicum L.) plant biomass through digital imaging using phenomics platform

  • R. H. Laxman
  • P. Hemamalini
  • R. M. Bhatt
  • A. T. Sadashiva
Original Article
  • 40 Downloads

Abstract

Phenotyping approaches, using high throughput imaging techniques, are being adopted over the traditional methodologies which are manpower intensive, time consuming and low throughput. However, the effectiveness of high throughput plant phenotyping through imaging in plant phenomics facility essentially requires establishing relationship between plant areas quantified through imaging and the actual biomass. The present study was conducted with an aim to standardise the methodology for digital quantification of tomato biomass using plant phenomics facility. A strong linear relationship was observed between the actual tomato plant fresh mass, digital biomass and projected shoot area. The correlations between plant fresh mass, plant digital biomass and projected shoot area were highly significant at 30, 45 and 60 days after transplanting, but at 75 days no correlation was observed. Hence, the present study clearly demonstrated that the growth of tomato plants could be monitored through digital imaging using either projected shoot area or digital biomass till 60 days after transplanting across genotypes for high throughput phenotyping.

Keywords

Plant phenomics Digital biomass Projected shoot area Digital imaging Image analysis 

Notes

Acknowledgements

This work is a part of the National Innovations in Climate Resilient Agriculture (NICRA) project funded by Indian Council of Agricultural Research, New Delhi. The authors are grateful to the Director, ICAR-Indian Institute of Horticultural Research, Bengaluru for all the support. We would also like to acknowledge Mr. Sridhar, C. for the technical assistance.

Supplementary material

40502_2018_374_MOESM1_ESM.docx (48 kb)
Supplementary material 1 (DOCX 48 kb)

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Copyright information

© Indian Society for Plant Physiology 2018

Authors and Affiliations

  • R. H. Laxman
    • 1
  • P. Hemamalini
    • 1
  • R. M. Bhatt
    • 1
  • A. T. Sadashiva
    • 2
  1. 1.Division of Plant Physiology and BiochemistryICAR-Indian Institute of Horticultural ResearchBangaloreIndia
  2. 2.Division of Vegetable CropsICAR-Indian Institute of Horticultural ResearchBangaloreIndia

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