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Adverse effects of heat stress in relation to actin cytoskeleton on pollen performance of Echinopsis chamaecereus (Cactaceae)

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Abstract

In this study, the effects of heat stress (30 °C, 35 °C, 40 °C) on pollen performance of Echinopsis chamaecereus Friedrich & Glaetzle were analyzed. Heat-treated pollen grains showed a insignificant reduction in germination rate. Pollen tubes treated by temperatures above 30 °C exhibited a decrease in tube length and a negative effect on pollen performance according to cumulative stress response index data. Pollen tube abnormality was enhanced by increased temperature, and bifurcated pollen tubes were very dominant than the other abnormalities. Callose accumulation on tube tips was enhanced by increased temperature. In bifurcated pollen tubes, one of the tubes blocked by callose plugs or dense callose accumulation was observed on tips. Actin organization showed disruption, and anisotropy of actin filaments increased at temperatures above 30 °C. The findings show that high temperatures cause destructive effects on male reproductive biology even in species such as cacti known to be temperature tolerant.

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Acknowledgements

This research did not receive any specific grant from funding agencies in the public, commercial or not-for-profit sectors.

Author information

AÇG conceived and planned the experiments and wrote the manuscript.

Correspondence to Aslıhan Çetinbaş-Genç.

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Çetinbaş-Genç, A. Adverse effects of heat stress in relation to actin cytoskeleton on pollen performance of Echinopsis chamaecereus (Cactaceae). Braz. J. Bot (2020). https://doi.org/10.1007/s40415-020-00580-0

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Keywords

  • Callose
  • Cell wall
  • High-temperature stress
  • Pollen tube elongation