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Brazilian Journal of Botany

, Volume 42, Issue 3, pp 431–439 | Cite as

Characterization of fatty acid components from Tetradesmus obliquus KNUA019 (Chlorophyta, Scenedesmaceae) for a resource of biofuel production

  • Young-Saeng Kim
  • Jung Yi
  • Jeong-Mi Do
  • Jiwon Chang
  • Ho-Sung YoonEmail author
Original Article
  • 32 Downloads

Abstract

Green algae produce fatty acid components that can be used in biofuel production without the need for additional nutrients. We aimed to elucidate the contribution of the T. obliquus KNUA019 through the control of the optimal culture (solvent, culture time, nitrogen, and phosphorus) conditions and thermal analysis (DTA and TGA curves) that affects fatty acid productivity maximum lipid yields from the four culture methods. Phylogenetic analysis of Tetradesmus obliquus (Turpin), Scenedesmus obliquus (Turpin), Acutodesmus obliquus (Turpin), and Chlorella sorokiniana (Shihira & R.W.Krauss) strains was attempted using the internal transcribed spacer. T. obliquus KNUA019 can produce significant amounts of carbon-containing components, which are valuable for use as energy sources. As a result of GC analysis, T. obliquus KNUA019 generates fatty acid components that are directly useful as biofuels, such as tetradecanoic acid (C14H28O2), methyl Z-11-tetradecenoate (C15H28O2), tetradecanoic acid (C15H30O2), 9-hexadecenoic acid (C15H30O2), pentadecane (C15H32), 8-heptadecene (C17H34), hexadecanoic acid (C17H34O2), heptadecane (C17H36), 9-octadecenoic acid (C19H36O2), octadecanoic acid (C19H38O2), and 3,7,11,15-tetramethyl-2-hexadecen-1-ol (C20H40O). These fatty acids can be used directly as biofuel precursors without transesterification. We indicate that commercial biofuel production is possible using mass culture of T. obliquus KNUA019, reducing production costs. This process was indicated as an optimum method for simplifying the process of fatty acid components under optimal culture conditions for a source of biofuels.

Keywords

Biomass Fatty acid Green algae Tetradesmus obliquus 

Notes

Acknowledgements

We thank Ji-Won Hong (National Marine Biodiversity Institute of Korea) and Kyoung-In Lee (Biotechnology Industrialization Center, Dongshin University, Korea) for helpful discussions and assisting with Materials and Methods. This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ01366701), Korea, and the Basic Science Research Program through the National Research Foundation of Korea (NRF) and funded by the Ministry of Education (2016R1A6A1A05011910; 2017R1A2B4002016; 2018R1D1A3B07049385), Korea.

Author contributions

YSK and HSY designed the experiments. JY, JMD, and JC performed the experiments. The article was written by YSK and edited by HSY. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

None of the authors has any financial or other relationships that could lead to a conflict of interest.

Supplementary material

40415_2019_556_MOESM1_ESM.pdf (19 kb)
Supplementary material 1 (PDF 18 kb)
40415_2019_556_MOESM2_ESM.docx (15 kb)
Supplementary material 2 (DOCX 14 kb)
40415_2019_556_MOESM3_ESM.docx (16 kb)
Supplementary material 3 (DOCX 15 kb)

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Copyright information

© Botanical Society of Sao Paulo 2019

Authors and Affiliations

  1. 1.Research Institute of Ulleung-do and Dok-doKyungpook National UniversityDaeguRepublic of Korea
  2. 2.Department of Biology, College of Natural SciencesKyungpook National UniversityDaeguRepublic of Korea
  3. 3.BK21 Plus KNU Creative BioResearch Group, School of Life SciencesKyungpook National UniversityDaeguRepublic of Korea

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