Brazilian Journal of Botany

, Volume 42, Issue 3, pp 431–439 | Cite as

Characterization of fatty acid components from Tetradesmus obliquus KNUA019 (Chlorophyta, Scenedesmaceae) for a resource of biofuel production

  • Young-Saeng Kim
  • Jung Yi
  • Jeong-Mi Do
  • Jiwon Chang
  • Ho-Sung YoonEmail author
Original Article


Green algae produce fatty acid components that can be used in biofuel production without the need for additional nutrients. We aimed to elucidate the contribution of the T. obliquus KNUA019 through the control of the optimal culture (solvent, culture time, nitrogen, and phosphorus) conditions and thermal analysis (DTA and TGA curves) that affects fatty acid productivity maximum lipid yields from the four culture methods. Phylogenetic analysis of Tetradesmus obliquus (Turpin), Scenedesmus obliquus (Turpin), Acutodesmus obliquus (Turpin), and Chlorella sorokiniana (Shihira & R.W.Krauss) strains was attempted using the internal transcribed spacer. T. obliquus KNUA019 can produce significant amounts of carbon-containing components, which are valuable for use as energy sources. As a result of GC analysis, T. obliquus KNUA019 generates fatty acid components that are directly useful as biofuels, such as tetradecanoic acid (C14H28O2), methyl Z-11-tetradecenoate (C15H28O2), tetradecanoic acid (C15H30O2), 9-hexadecenoic acid (C15H30O2), pentadecane (C15H32), 8-heptadecene (C17H34), hexadecanoic acid (C17H34O2), heptadecane (C17H36), 9-octadecenoic acid (C19H36O2), octadecanoic acid (C19H38O2), and 3,7,11,15-tetramethyl-2-hexadecen-1-ol (C20H40O). These fatty acids can be used directly as biofuel precursors without transesterification. We indicate that commercial biofuel production is possible using mass culture of T. obliquus KNUA019, reducing production costs. This process was indicated as an optimum method for simplifying the process of fatty acid components under optimal culture conditions for a source of biofuels.


Biomass Fatty acid Green algae Tetradesmus obliquus 



We thank Ji-Won Hong (National Marine Biodiversity Institute of Korea) and Kyoung-In Lee (Biotechnology Industrialization Center, Dongshin University, Korea) for helpful discussions and assisting with Materials and Methods. This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ01366701), Korea, and the Basic Science Research Program through the National Research Foundation of Korea (NRF) and funded by the Ministry of Education (2016R1A6A1A05011910; 2017R1A2B4002016; 2018R1D1A3B07049385), Korea.

Author contributions

YSK and HSY designed the experiments. JY, JMD, and JC performed the experiments. The article was written by YSK and edited by HSY. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

None of the authors has any financial or other relationships that could lead to a conflict of interest.

Supplementary material

40415_2019_556_MOESM1_ESM.pdf (19 kb)
Supplementary material 1 (PDF 18 kb)
40415_2019_556_MOESM2_ESM.docx (15 kb)
Supplementary material 2 (DOCX 14 kb)
40415_2019_556_MOESM3_ESM.docx (16 kb)
Supplementary material 3 (DOCX 15 kb)


  1. Alberts B, Johnson A, Lewis J et al (2003) Molecular biology of the cell. Ann Bot 91:401CrossRefGoogle Scholar
  2. Bligh E, Dyer W (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917CrossRefPubMedPubMedCentralGoogle Scholar
  3. Buchheim MA, Keller A, Koetschan C et al (2011) Internal transcribed spacer 2 (nu ITS2 rRNA) sequence-structure phylogenetics: towards an automated reconstruction of the green algal tree of life. PLoS ONE 6:e16931CrossRefPubMedPubMedCentralGoogle Scholar
  4. Chang J, Hong JW, Chae H et al (2013) Natural production of alkane by an easily harvested freshwater cyanobacterium, Phormidium autumnale KNUA026. Algae 28:93–99CrossRefGoogle Scholar
  5. Choi GG, Bae MS, Ahn CY, Oh HM (2008) Induction of axenic culture of Arthrospira (Spirulina) platensis based on antibiotic sensitivity of contaminating bacteria. Biotechnol Lett 30:87–92CrossRefPubMedGoogle Scholar
  6. Domozych DS, Domozych CE (2014) Multicellularity in green algae: upsizing in a walled complex. Front Plant Sci 5:649CrossRefPubMedPubMedCentralGoogle Scholar
  7. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
  8. Furtado ALFF, do Carmo Calijuri M, Lorenzi AS et al (2009) Morphological and molecular characterization of cyanobacteria from a Brazilian facultative wastewater stabilization pond and evaluation of microcystin production. Hydrobiologia 627:195–209CrossRefGoogle Scholar
  9. He M, Yan Y, Pei F et al (2017) Improvement on lipid production by Scenedesmus obliquus triggered by low dose exposure to nanoparticles. Sci Rep 7:15526CrossRefPubMedPubMedCentralGoogle Scholar
  10. Hegewald E, Wolf M (2003) Phylogenetic relationships of Scenedesmus and Acutodesmus (Chlorophyta, Chlorophyceae) as inferred from 18S rDNA and ITS-2 sequence comparisons. Plant Syst Evol 241:185–191CrossRefGoogle Scholar
  11. Hoydonckx HE, De Vos DE, Chavan SA et al (2004) Esterification and transesterification of renewable chemicals. Top Catal 27:83–96CrossRefGoogle Scholar
  12. Hu Q, Sommerfeld M, Jarvis E et al (2008) Microalgal triacyglycerols as feedstocks for biofuel production: perspective and advances. Plant J 54:621–639CrossRefPubMedPubMedCentralGoogle Scholar
  13. Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mammalian protein metabolism. Academic Press, New York, pp 21–132CrossRefGoogle Scholar
  14. Kang B, Yoon HS (2015) Thermal analysis of green algae for comparing relationship between particle size and heat evolved. Biomass Conv Biorefin 5:279–285CrossRefGoogle Scholar
  15. Khattar JIS, Singh DP, Jindal N, Kaur N, Singh Y, Rahi P, Gulati A (2010) Isolation and characterization of exopolysaccharides produced by the cyanobacterium Limnothrix redekei PUPCCC 116. Biotechnol Appl Biochem 162:1327–1338CrossRefGoogle Scholar
  16. Kim SK, Baek HC, Byun HG et al (2001) Biochemical composition and antioxidative activity of marine microalgae. J Korean Fish Soc 34:260–267Google Scholar
  17. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  18. Li X, Hu HY, Gan K et al (2010) Effects of different nitrogen and phosphorus concentrations on the growth, nutrient uptake, and lipid accumulation of a freshwater microalga Scenedesmus sp. Biores Technol 101:5494–5500CrossRefGoogle Scholar
  19. Lin Q, Lin J (2011) Effects of nitrogen source and concentration on biomass and oil production of a Scenedesmus rubescens like microalga. Biores Technol 102:1615–1621CrossRefGoogle Scholar
  20. Loza V, Berrendero E, Perona E et al (2013) Polyphasic characterization of benthic cyanobacterial diversity from biofilms of the Guadarrama river (Spain): morphological, molecular, and ecological approaches. J Phycol 49:282–297CrossRefPubMedGoogle Scholar
  21. Ma F, Hanna MA (1999) Biodiesel production: a review. Biores Technol 70:1–15CrossRefGoogle Scholar
  22. Mandal S, Mallick N (2009) Microalga Scenedesmus obliquus as a potential source for biodiesel production. Microbiol Biotechnol 84:281–291CrossRefGoogle Scholar
  23. Martinez ME, Jimenez JM, El Yousfi F (1999) Influence of phosphorus concentration and temperature on growth and phosphorus uptake by the microalga Scenedesmus obliquus. Biores Technol 67:233–240CrossRefGoogle Scholar
  24. Mercer P, Armenta RE (2011) Developments in oil extraction from microalgae. Eur J Lipid Sci Technol 113:539–547CrossRefGoogle Scholar
  25. Nelson DL, Cox MM (2000) Lehninger, principles of biochemistry, 3rd edn. Worth Publishing, New York. ISBN 1-57259-153-6Google Scholar
  26. Ochiai M, Ozao R (1992) Thermal analysis and self-similarity law in particle size distribution of powder sample: part I. Thermochim Acta 198:279–287CrossRefGoogle Scholar
  27. Razzaque MS (2011) Phosphate toxicity: new insights into an old problem. Clin Sci (Lond) 120:91–97CrossRefGoogle Scholar
  28. Rosenberg JN, Kobayashi N, Barnes A et al (2014) Comparative analyses of three Chlorella species in response to light and sugar reveal distinctive lipid accumulation patterns in the Microalga C. sorokiniana. PLoS One 9:e92460CrossRefPubMedPubMedCentralGoogle Scholar
  29. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  30. Sharif Hossain ABM, Salleh A, Boyce AN et al (2008) Biodiesel fuel production from algae as renewable energy. Am J Biochem Biotechnol 4:250–254CrossRefGoogle Scholar
  31. Somashekar D, Venkateshwaran G, Srividya C et al (2001) Efficacy of extraction methods for lipid and fatty acid composition from fungal cultures. World J Microbiol Biotechnol 17:317–320CrossRefGoogle Scholar
  32. Taton A, Grubisic S, Ertz D et al (2006) Polyphasic study of Antarctic cyanobacterial strains. J Phycol 42:1257–1270CrossRefGoogle Scholar
  33. Vance C (2001) Symbiotic nitrogen fixation and phosphorus acquisition. Plant nutrition in a world of declining renewable resources. Plant Physiol 127:391–397CrossRefGoogle Scholar
  34. Widjaja A, Chien CC, Ju YH (2009) Study of increasing lipid production from fresh water microalgae Chlorella vulgaris. J Taiwan Inst Chem E 40:13–20CrossRefGoogle Scholar
  35. Wingender J, Neu TR, Flemming HC (1999) What are bacterial extracellular polymeric substances? In: Wingender J, Neu TR, Flemming HC (eds) Microbial extracellular polymeric substances. Springer, Berlin, Heidelberg, pp 1–19. CrossRefGoogle Scholar
  36. Wynne MJ, Hallan JK (2016) Reinstatement of Tetradesmus G. M. Smith (Sphaeropleales, Chlorophyta). Feddes Rep 126:83–86CrossRefGoogle Scholar
  37. Yeo H, Youn K, Kim M et al (2013) Fatty acid composition and volatile constituents of protaetia brevitarsis larvae. Prev Nutr Food Sci 18:150–156CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Botanical Society of Sao Paulo 2019

Authors and Affiliations

  1. 1.Research Institute of Ulleung-do and Dok-doKyungpook National UniversityDaeguRepublic of Korea
  2. 2.Department of Biology, College of Natural SciencesKyungpook National UniversityDaeguRepublic of Korea
  3. 3.BK21 Plus KNU Creative BioResearch Group, School of Life SciencesKyungpook National UniversityDaeguRepublic of Korea

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