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Brazilian Journal of Botany

, Volume 42, Issue 3, pp 407–419 | Cite as

Storage time affects the germination and proteomic profile of seeds of Cariniana legalis (Mart.) O. Kuntze (Lecythidaceae), an endangered tree species native to the Brazilian Atlantic Forest

  • Victor Paulo Mesquita Aragão
  • Bianca Machado Campos Trindade
  • Ricardo Souza Reis
  • Vanildo Silveira
  • Claudete Santa-CatarinaEmail author
Original Article
  • 54 Downloads

Abstract

Seed aging is a critical process during storage and plays an important role in maintaining seed vigor. The present study aimed to investigate the effects of seed storage on germination and the differential abundance of proteins associated with viability loss in Cariniana legalis (Mart.) O. Kuntze. Seeds were stored for 12 months at 6 °C in plastic bags. Analyses of germination were performed at time 0 (newly harvested seeds) and after 3, 6, and 12 months of storage. To identify differentially abundant proteins, two-dimensional electrophoresis was performed, followed by tandem mass spectrometric (MS/MS) analysis. Germination, the germination speed index and the moisture content were significantly reduced during storage. Proteomic analysis revealed 50 differentially abundant proteins among the times analyzed; these proteins were classified into several functional classes related to the biological, metabolic, cellular, oxidation–reduction, carbohydrate metabolism, storage, aging, cell wall organization or biogenesis, response to stress and stimulus, and proteolysis categories. The correlation between seed aging and proteome changes suggests that specific proteins may be involved in the mechanisms underlying seed deterioration, including ferritin, seed maturation-like, 2,4-dienoyl-CoA reductase, 11S globulin, polygalacturonases, superoxide dismutase, heat shock proteins, and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Our data showed that increasing storage time significantly reduced germination and revealed the regulation of differentially abundant proteins, which may be related to the reduction in seed viability in C. legalis.

Keywords

Protein abundance Seed longevity Two-dimensional gel electrophoresis Viability Woody species 

Notes

Acknowledgements

Funding for this work was provided by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (444453/2014-8 and 307596/2016-8) and the Fundação Carlos Chagas Filho de Amparo à Pesquisa no Estado do Rio de Janeiro (FAPERJ) (E26/111.389-2012, E26/010.001507/2014, and E26/202.969/2016). This study was also financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES)—Finance Code 001. VPMA is thankful for the PhD scholarship provided by CAPES and for the postdoctoral fellowship provided by FAPERJ (proc. E-26/202.295/2017).

Author contributions

CSC, VS, and VPMA conceived the study, designed the experiments, and wrote the manuscript. VPMA and BMCT were responsible for the preparation of protein samples, gels, image analysis, and preparation of samples for mass spectrometry. RSR collaborated on the mass spectrometry analysis. All of the authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Botanical Society of Sao Paulo 2019

Authors and Affiliations

  1. 1.Laboratório de Biologia Celular e Tecidual (LBCT), Centro de Biociências e Biotecnologia (CBB)Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Campos dos GoytacazesBrazil
  2. 2.Laboratório de Biotecnologia (LBT), CBBUENFCampos dos GoytacazesBrazil
  3. 3.Unidade de Biologia Integrativa, Setor de Genômica e ProteômicaUENFCampos dos GoytacazesBrazil

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