Tupistra nutans Wall. root extract, rich in phenolics, inhibits microbial growth and α-glucosidase activity, while demonstrating strong antioxidant potential

  • Ill-Min Chung
  • Ramachandran Chelliah
  • Deog-Hwan Oh
  • Seung-Hyun Kim
  • Chang Yeon Yu
  • Bimal Kumar GhimireEmail author
Original Article


The current study was performed to determine the phenolic compounds composition, antioxidant and antimicrobial activity, and α-glucosidase inhibitory effect of the root extract of Tupistra nutans Wall., in order to validate its use in traditional medicine. HPLC–MS/MS analysis showed the presence of ferulic acid, protocatechuic acid, p-hydroxybenzoic acid, p-coumaric acid, salicylic acid, chlorogenic acid, caffeic acid, l-phenylalanine as the predominant phenolic compounds. The antioxidant potentials of T. nutans were determined in different solvent extract and found to vary in a concentration-dependent manner. Ethyl acetate-soluble fraction exhibited the highest amount of phenolic acids, flavonoids, antioxidant activity against the DPPH, reduced Fe3+/ferric cyanide complexes to the ferrous form and inhibitory effect on α-glucosidase. Butanol fraction showed the strongest ABTS radical scavenging ability. Methanolic root extract showed higher reduction capability of ferric ions than the other solvent fractions. A significant and higher positive correlation was found between total phenolic content and total flavonoid content with α-glucosidase inhibition and DPPH using ethyl acetate solvent than the other solvent fractions. All the tested microorganisms: Staphylococcus aureus Rosenback (ATCC13150), Salmonella enterica typhimurium Kauffmann and Edwads (ATCC14028), Escherichia coli Castllani and Chalmers (ATCC35150), and fungi: Candida albicans Robin and Berkhout (KTCC7965), Aspergillus fumigatus Fresenius (KTCC6145), A. flavus var. flavus Link ex Fries (KTCC6143), A. niger van Tieghem (KTCC6317) were susceptible to the root extracts at a concentration of 0.12–0.25 mg mL−1. This study may allow us to understand indigenous medicinal values of T. nutans. Furthermore, these results showed that T. nutans is a good source of antioxidants along with antimicrobial and antidiabetic activities and could be used as an important bioresource for the pharmaceutical and food industries.


Diabetes mellitus Flavonoids Microbial growth Nakima Phenolics Phytochemicals Tupistra nutans 



This study was supported by the KU Research Professor program.

Author contributions

BKG performed designing of experiment and writing of manuscript. CYY supervised the experiment. RC and D-HO performed the antimicrobial activities. S-HK and I-MC performed the phytochemical determination, analysis and editing of manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Botanical Society of Sao Paulo 2019

Authors and Affiliations

  • Ill-Min Chung
    • 1
  • Ramachandran Chelliah
    • 3
  • Deog-Hwan Oh
    • 3
  • Seung-Hyun Kim
    • 1
  • Chang Yeon Yu
    • 2
  • Bimal Kumar Ghimire
    • 1
    Email author
  1. 1.Department of Applied Life ScienceKonkuk UniversitySeoulSouth Korea
  2. 2.Bioherb Research InstituteKangwon National UniversityChuncheonSouth Korea
  3. 3.Department of Food Science and Biotechnology, College of Agriculture and Life SciencesKangwon National UniversityChuncheonSouth Korea

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