Constitutive H2O2 is involved in sorghum defense against aphids

  • Yutao ShaoEmail author
  • Mingxin Guo
  • Xianfeng He
  • Qingxin Fan
  • Zhenjie Wang
  • Jin Jia
  • Jiangbo Guo
Original Article


Reactive oxygen species (ROS) play a major role in plant defense against pathogens, but the evidence for their role in defense against insects is still limited. In this study, HN16 and its mutated line (asm1) were used as research objects, and the potential role of H2O2 in sorghum against aphid was examined. Constitutive H2O2 was considered the main factor associated with increased aphid tolerance, although aphid feeding also induced an increase in the H2O2 concentration. By studying ROS scavenging enzymes, it was found that APX and GPX were closely related, but SOD, POX and CAT were not involved in RMES1-mediated resistance. DEGs involved in the detoxification of ROS in HN16 worked through different means. Analysis of three DEGs encoding APX and GPX revealed that although the expression changes of SbAPx1 were consistent and those of SbGPx1 and SbGPx2 were inconsistent with enzyme activity, they all played an important role in RMES1-mediated resistance to aphids.


Antioxidant enzymes Melanaphis sacchari Reactive oxygen species 



Ascorbate peroxidase


One-way analysis of variance




Differentially expressed gene




Glutathione peroxidase




Glutathione S-transferases


Hydrogen peroxide




Reactive oxygen species


Superoxide dismutase





This study was supported by grants from the National Natural Science Foundation of China (31660396), the Natural Science Foundation of Inner Mongolia (2016MS0308), the Innovation Fund (Excellent Youth Science Fund) of Inner Mongolia University of Science and Technology (2017YQL09) and the Innovation Fund for College Students of Inner Mongolia University of Science and Technology (2015016).

Authors’ contributions

YS, JJ and JG contributed to the experimental design and writing of this manuscript. MG, XH and QF contributed to the performance of experiments. ZW contributed to the data analysis.


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Copyright information

© Botanical Society of Sao Paulo 2019

Authors and Affiliations

  1. 1.School of Life Science and TechnologyInner Mongolia University of Science and TechnologyBaotouPeople’s Republic of China

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