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Brazilian Journal of Botany

, Volume 42, Issue 1, pp 97–105 | Cite as

A CkDREB1 gene isolated from Caragana korshinskii Kom. enhances Arabidopsis drought and cold tolerance

  • Ziyi Zhang
  • Qi Yang
  • Chunlin Zhang
  • Lili Wei
  • Rong Yue
  • Guojing Li
  • Xiaofei LinEmail author
  • Ruigang WangEmail author
Original Article
  • 64 Downloads

Abstract

Caragana korshinskii Kom., an arbuscular legume with important economic and ecological value in feed, processing industry, and environmental protection, also has great tolerance potential to abiotic stress conditions. An AP2 domain-containing gene was isolated from the suppression subtractive hybridization library of C. korshinskii under drought stress. In addition, the isolated gene was also found to be responsive to cold and ABA treatment. Phylogenetic analysis indicates that the deduced protein belongs to the DREB A-1 subfamily and is designated as CkDREB1. Overexpression of CkDREB1 in Arabidopsis thaliana (L.) Heynh increased drought and cold tolerance compared with the wild type. The drought responsive genes RD29A, RD29B, KIN1, and KIN2, as well as cold-responsive marker genes COR15A and COR47, were also highly induced in the overexpression lines under drought and cold conditions. These results should shed light on our understanding on the mechanisms of abiotic resistance of C. korshinskii.

Keywords

AP2 DNA-binding motif DREB Drought stress Transcription activator 

Notes

Acknowledgements

We thank Dr. Mark Goettel, the Editor-in-Chief of Biocontrol Science and Technology, for polishing the manuscript carefully. This work was supported by the National Natural Science Foundation of China (No. 31060105 and No. 31360169), grants from Chinese National Programs for High Technology Research and Development (No. 2011AA100203), and Inner Mongolia Natural Science Foundation (2010Zd13).

Author contributions

RW and XL conceived of the research, and RW designed the study and wrote the manuscript. ZZ, QY, and CZ conducted the most of the experiments. LW performed all of the plasmid construction. RY completed the plant transformation and provide plant materials. GL did bioinformatics analysis.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

40415_2018_509_MOESM1_ESM.doc (1.8 mb)
Supplementary material 1 (DOC 1882 kb)

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Copyright information

© Botanical Society of Sao Paulo 2019

Authors and Affiliations

  1. 1.Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology, College of Life SciencesInner Mongolia Agricultural UniversityHohhotPeople’s Republic of China
  2. 2.Key Laboratory of Forage and Endemic Crop Biotechnology, Ministry of Education, School of Life SciencesInner Mongolia UniversityHohhotPeople’s Republic of China

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