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Brazilian Journal of Botany

, Volume 42, Issue 1, pp 195–200 | Cite as

Genome survey and development of 15 microsatellite molecular markers in Syagrus coronata (Martius) Beccari (Arecaceae) by next-generation sequencing

  • Suzanne Nunes Barbosa
  • Cicero AlmeidaEmail author
Short Communication
  • 30 Downloads

Abstract

Syagrus coronata (Martius) Beccari belongs to the Arecaceae family, an economically and ecologically important species of palm tree. It grows in northeastern Brazil and has a widespread geographic distribution in the “Caatinga” (a seasonally dry tropical forest biome) and Atlantic forest domains (a rain forest biome). The aim of this study was to perform a genome survey and develop SSR markers using high-throughput sequencing. We obtained 78,231,881 million Illumina single-end and paired-end reads, and these reads were used to obtain contigs with a minimum of 500 nucleotides using Ray software. Mining of the SSRs was performed with Phobos software, while the primers were designed by Primer3. Thirty loci were analyzed, three of which were not amplified, 12 were monomorphic, and 15 were polymorphic. Five to 11 alleles per locus were observed, with the observed and expected heterozygosity at 0.06–0.63 and 0.73–0.88, respectively. Here, we report the first set of microsatellite markers developed for S. coronata, and their polymorphism levels indicate they can be used as powerful tools for evaluating genetic diversity and population structure, and thus contribute to conservation studies.

Keywords

Arecaceae Genetic resources Simple sequence repeat 

Notes

Acknowledgements

We acknowledge the Federal University of Alagoas for the laboratories, the financial support of Fundação de Apoio à Pesquisa de Alagoas (FAPEAL), and the National Council for the Improvement of Higher Education (CAPES).

Compliance with ethical standards

Author contributions

Barbosa SN performed the experiments, and Almeida C analyzed the data and wrote the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

40415_2018_507_MOESM1_ESM.tif (16.5 mb)
Fig. S1 Number of repeats by all motifs and di- to hexanucleotides motifs (TIFF 16899 kb)

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Copyright information

© Botanical Society of Sao Paulo 2018

Authors and Affiliations

  1. 1.Laboratório de Recursos Genéticos, Campus de ArapiracaUniversidade Federal de AlagoasArapiracaBrazil

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