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Brazilian Journal of Botany

, Volume 41, Issue 4, pp 889–900 | Cite as

Diversity of Chondracanthus (Gigartinaceae, Rhodophyta) on the Brazilian coast based on molecular and morphological evidences

  • Renato Rocha-Jorge
  • Fábio Nauer
  • Ingrid B. Silva
  • Mutue T. Fujii
  • Orlando Necchi Jr.
  • Line Le Gall
  • Mariana C. Oliveira
Original Article
  • 57 Downloads

Abstract

Based on morphological studies, four species of Chondracanthus Kützing were previously reported on the Brazilian coast: C. acicularis (Roth) Fredericq, C. elegans (Greville) Guiry, C. saundersii C.W.Schneider and C.E.Lane and C. teedei (Mertens ex Roth) Fredericq. In the present study, specimens of Chondracanthus were collected from intertidal and subtidal zones (up to 18 m depth); morphological, molecular and phylogenetic analyses were performed using DNA barcoding (cox1) as a tool in a survey of Rhodophyta biodiversity in São Paulo state. Unlike what we expected, C. saundersii is widely distributed, and many specimens were misidentified as C. acicularis or C. teedei before this study, suggesting the necessity of an accurate revision in the previous identifications of the species of Chondracanthus in Brazil. Tetrasporangial and cystocarpic structures of C. saundersii are described for the first time. Our results confirm that the utilization of cox1 sequences as DNA barcode is an efficient tool to complement morphological identification for species of the genus Chondracanthus.

Keywords

coxPhylogeny rbcTaxonomy 

Abbreviations

cox1

Cytochrome c oxidase

GTR

General-time-reversible model invariable sites and shape parameter of the gamma distribution

MP

Maximum parsimony

NJ

Neighbor-joining

PP

Posterior probability

rbcL

Gene coding for the large subunit of RUBISCO

Notes

Acknowledgements

We thank the technical help from Carolina de Oliveira Franco. We acknowledge Fundação de Amparo à Pesquisa no Estado de São Paulo (FAPESP, BIOTA Proc. 07/51270-7; 2016/50370-7) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq Proc. 484647/2012-1; 440523/2015-0) for the project financial support. RRJ thanks FAPESP for PhD Fellowship (Proc. 2010/20559-4). MCO, MTF and ONJ thank CNPq for Research Productivity Fellowships; This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Brasil (CAPES) Finance Code 001. Molecular data from French specimens were produced at the Service de Systématique Moléculaire of the Muséum National d’Histoire Naturelle (CNRS - UMS 2700) with funds provided by the ATM ‘Taxonomie moléculaire: DNA Barcode et gestion durable des collections.’ Sequencing was performed by Genoscope through the projects entitled ‘Speed ID’ and ‘Bibliothèque du Vivant.’

Author Contribution Statement

RRJ, MTF, FN, IBS and MCO conducted field work. RRJ and MTF conducted taxonomic work, gathered and analyzed data. FN sequenced specimens and conducted molecular biology work. FN, ONJ, LLG and MCO gathered data and conducted phylogenetic analysis. MTF and MCO supervised the study and contributed to the discussion. All authors were involved in writing and discussion of the manuscript.

Supplementary material

40415_2018_501_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 14 kb)

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Copyright information

© Botanical Society of Sao Paulo 2018

Authors and Affiliations

  1. 1.Botanical InstituteSão PauloBrazil
  2. 2.Department of BotanyInstitute of Biosciences, University of São PauloSão PauloBrazil
  3. 3.Zoology and Botany DepartmentSão Paulo State UniversityS. José Rio PretoBrazil
  4. 4.Institut Systématique Evolution Biodiversité (ISYEB), Muséum national d’Histoire naturelle, CNRS, Sorbonne University, EPHEParisFrance

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