Effect of silver diamine fluoride on adhesion and microleakage of a pit and fissure sealant to tooth enamel: in vitro trial
- 180 Downloads
To identify the effect of silver diamine fluoride in adhesion and microleakage of a pit and fissure sealant to tooth enamel.
An in vitro study including 120 human molars without caries, and caries grade 1 was performed. Molars were randomly divided into four groups. Occlusal surfaces of groups 2 and 4 were sealed. In groups 1 and 3 silver diamine fluoride (SDF) was placed in the occlusal surface and then molars were sealed according to manufacturer’s instructions. Afterwards, all groups were submitted to the adhesion test. Groups 1 and 2 were placed in a forming cylinder and their roots were submerged in polymethyl methacrylate after that they were stored in deionised water (37 °C ± 1). Subsequently, they were tested by applying a tractional load (Instron machine, speed cross of 1 mm/min). Sealant adhesion was evaluated through: Resistance to the detachment (MPa), residues of sealant area (mm2) and adhesive remaining index (ARI) (0–3 interval). Microleakage test: Groups 3 and 4 were placed in a plastic container and subjected to 2000 cycles at different temperatures one at 6 °C ± 1 and the other at 60 °C ± 1. Later the occlusal surface was immersed for 24 h in 1% toluidine blue. Microleakage was observed by optical microscopy (10×) and was interpreted in micrometres.
Of the samples without SDF 18.1 and 23.0% of the sealant remaining and of the samples with SDF present 100% of sealant remaining on enamel. Samples with SDF obtained an average in the test of detachment of 6.89 ± 3.09 MPa, whereas in the group without SDF the average obtained was 6.42 ± 3.35 MPa. Differences were not statistically significant (p = 0.579). Most of the samples not treated with SDF (81.6%) showed microleakage than those treated with SDF in which 47% presented microleakage.
There is an improvement in the retention properties of a fissure sealant applied after treatment with silver diamine fluoride. The application of fissure sealant to improve the aesthetics of teeth treated with SDF is recommended.
KeywordsPit and fissure sealant Silver diamine fluoride Adhesion Microleakage
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Clu C, Lo E. Promoting caries arrest in children with silver diamine fluoride. Oral Health Prev Dent. 2015;6:315–21.Google Scholar
- Futatsuky M, Kubota K, Yeh YC, et al. Early loss of pit and fissure sealant: a clinical and SEM study. J Clin Pediatr Dent. 1995;19:99–104.Google Scholar
- Joshi K, Dave B, Joshi N, et al. Comparative evaluation of two different pit and fissure sealants and a restorative material to check their microleakage- an in vitro study. J Int Oral Heath. 2013;5:35–9.Google Scholar
- Kenneth J. Anusavice. Ciencia de los materiales dentales. Madrid: Elsever; 2004. pp. 53–5.Google Scholar
- Shama G, Puranik MP. KRS. Approaches to arresting dental caries: an update. J Clin Diagn Res. 2015;9:8–11.Google Scholar
- Uzuel I, Ulukent O, Cogulu D. The effect of silver diamine fluoride on microleakage of resin composite. J Int Dent Med Res. 2013;6:105–8.Google Scholar
- World Health Organization. Oral health surveys. Basic methods. 3rd. Suiza: Geneve; 1987.Google Scholar