European Archives of Paediatric Dentistry

, Volume 19, Issue 3, pp 139–145 | Cite as

Dental plaque disclosure as an auxiliary method for infants’ oral hygiene

  • C. A. B. Cardoso
  • N. M. Santos
  • M. L. C. Fracasso
  • M. G. A. Provenzano
  • T. M. Oliveira
  • D. RiosEmail author
Original Scientific Article



This was to evaluate the effectiveness of plaque disclosure as an auxiliary method for early childhoods’ oral hygiene.


The study was performed with 20 mothers and their children (aged 6–36 months), members of a preventive programme, which two groups used one of two approaches: conventional oral hygiene/group I (tooth brushing) and plaque disclosure with subsequent oral hygiene/group II (tooth brushing with prior plaque disclosure). Ten mothers started the study in group I and the other 10 in group II, after one month interval they changed to be in the alternate group. Each group consisted of baseline and three additional visits at weekly intervals. The effectiveness of oral hygiene was assessed in a blind fashion by plaque quantity estimation, using the Green Vermilion index for smooth surfaces and the plaque thickness index for occlusal surfaces. Statistical comparisons were performed using repeated measures ANOVA/Fisher’s post hoc test and paired t-test (p < 0.05).


For smooth and occlusal surfaces at first and second visits, group II recorded significantly lower plaque indices when compared with group I. Additionally, when considering the mean dental plaque index of all visits, group II also presented lower plaque scores than group I.


Dental plaque disclosure before toothbrushing helps mothers to enhance the effectiveness of early childhood oral hygiene.

Registration number and name of trial registry

RBR-7fyc7g; Avaliação do Treinamento e de Métodos Auxiliares na Efetividade da Escovação Dentária Materna em Bebês. Where the full trial protocol can be accessed:


Oral hygiene Early childhood Dental plaque 



The authors acknowledge the valuable collaboration of all mothers and their infants involved in this study. The authors also acknowledge Bitufo for the donation of toothbrushes.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© European Academy of Paediatric Dentistry 2018

Authors and Affiliations

  1. 1.Graduate Program in DentistryCruzeiro do Sul UniversitySão PauloBrazil
  2. 2.Department of Pediatric Dentistry, Orthodontics and Community HealthBauru School of Dentistry,BauruBrazil
  3. 3.Department of Pediatric Dentistry and Orthodontics, Dental SchoolMaringa State UniversityMaringáBrazil

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