European Archives of Paediatric Dentistry

, Volume 19, Issue 1, pp 57–60 | Cite as

Salivary microbial profiles in 5-year old children with oral clefts: a comparative study

  • A. L. SundellEmail author
  • C. Ullbro
  • G. Dahlén
  • A. Marcusson
  • S. Twetman
Original Scientific Article



To compare the salivary microbial profile in children with oral clefts to matched non-cleft controls in a cross-sectional study.


The cleft group consisted of 80 children aged 5 years and 144 age-matched non-cleft children were recruited as a control group. Stimulated whole saliva samples were collected and analysed with checkerboard DNA–DNA hybridisation using 12 pre-determined bacterial probes. Background data on caries, oral hygiene, dietary habits and fluoride exposure were collected from clinical examinations and questionnaires.


Children in the cleft group had a significantly higher prevalence of dental caries but only minor differences in the salivary microbial profile were found. In general, the cleft-children displayed a lower prevalence of common commensal species (e.g. Streptococcus, Fusobacterium) when compared with the non-cleft controls. The differences were statistically significant (p < 0.05) but the clinical significance was unclear.


The profile of the salivary microflora in 5-year-old children with and without oral clefts was basically similar and displayed only marginal differences with respect to commensal bacteria.


Salivary microflora Caries Oral hygiene Cleft lip and palate Children 



The authors would like to thank Mrs Susanne Blomqvist, Section for Oral Microbiology, Institute of Odontology at Sahlgrenska Academy at University of Gothenburg, for her skilled technical assistance. This work was supported by Region Jönköping County, Medical Research Council of Southeast Sweden (FORSS), Academy for Health and Care Region Jönköping County (Futurum), the Swedish Dental Association, and the Swedish Society of Paediatric Dentistry (SSPD).

Compliance with ethical standards

Conflict of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.


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Copyright information

© European Academy of Paediatric Dentistry 2018

Authors and Affiliations

  • A. L. Sundell
    • 1
    Email author
  • C. Ullbro
    • 2
  • G. Dahlén
    • 3
  • A. Marcusson
    • 4
  • S. Twetman
    • 5
  1. 1.Department of Paediatric DentistryInstitute for Postgraduate Dental EducationJönköpingSweden
  2. 2.Department of Clinical DentistryThe Arctic University of NorwayTromsøNorway
  3. 3.Department of Oral Microbiology and ImmunologySahlgrenska Academy at the University of GothenburgGothenburgSweden
  4. 4.Dentofacial Orthopedics, Maxillofacial UnitLinköping University HospitalLinköpingSweden
  5. 5.Department of Odontology, Section for Cariology and Endodontics, Faculty of Health and Medical SciencesUniversity of CopenhagenCopenhagenDenmark

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