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European Archives of Paediatric Dentistry

, Volume 19, Issue 1, pp 57–60 | Cite as

Salivary microbial profiles in 5-year old children with oral clefts: a comparative study

  • A. L. SundellEmail author
  • C. Ullbro
  • G. Dahlén
  • A. Marcusson
  • S. Twetman
Original Scientific Article
First Online:

Abstract

Aim

To compare the salivary microbial profile in children with oral clefts to matched non-cleft controls in a cross-sectional study.

Methods

The cleft group consisted of 80 children aged 5 years and 144 age-matched non-cleft children were recruited as a control group. Stimulated whole saliva samples were collected and analysed with checkerboard DNA–DNA hybridisation using 12 pre-determined bacterial probes. Background data on caries, oral hygiene, dietary habits and fluoride exposure were collected from clinical examinations and questionnaires.

Results

Children in the cleft group had a significantly higher prevalence of dental caries but only minor differences in the salivary microbial profile were found. In general, the cleft-children displayed a lower prevalence of common commensal species (e.g. Streptococcus, Fusobacterium) when compared with the non-cleft controls. The differences were statistically significant (p < 0.05) but the clinical significance was unclear.

Conclusions

The profile of the salivary microflora in 5-year-old children with and without oral clefts was basically similar and displayed only marginal differences with respect to commensal bacteria.

Keywords

Salivary microflora Caries Oral hygiene Cleft lip and palate Children 
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Notes

Acknowledgements

The authors would like to thank Mrs Susanne Blomqvist, Section for Oral Microbiology, Institute of Odontology at Sahlgrenska Academy at University of Gothenburg, for her skilled technical assistance. This work was supported by Region Jönköping County, Medical Research Council of Southeast Sweden (FORSS), Academy for Health and Care Region Jönköping County (Futurum), the Swedish Dental Association, and the Swedish Society of Paediatric Dentistry (SSPD).

Compliance with ethical standards

Conflict of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

References

  1. Aas JA, Griffen AL, Dardis SR et al. Bacteria of dental caries in primary and permanent teeth in children and young adults. J Clin Microbiol. 2008;46(4):1407–17.CrossRefPubMedPubMedCentralGoogle Scholar
  2. Ahluwalia M, Brailsford SR, Tarelli E et al. Dental caries, oral hygiene, and oral clearance in children with craniofacial disorders. J Dent Res. 2004;83(2):175–9.CrossRefPubMedGoogle Scholar
  3. Antonarakis GS, Palaska PK, Herzog G. Caries prevalence in non-syndromic patients with cleft lip and/or palate: a meta-analysis. Caries Res. 2013;47(5):406–13.  https://doi.org/10.1159/000349911.CrossRefPubMedGoogle Scholar
  4. Antunes LS, Tannure PN, Antunes LA et al. Genetic association for caries susceptibility among cleft lip and/or palate individuals. J Contemp Dent Pract. 2014;15(3):288–93.CrossRefPubMedGoogle Scholar
  5. Cheng LL, Moor SL, Kravchuk O, Meyers IA, Ho CT. Bacteria and salivary profile of adolescents with and without cleft lip and/or palate undergoing orthodontic treatment. Aust Dent J. 2007;52(4):315–21.CrossRefPubMedGoogle Scholar
  6. Chopra A, Lakhanpal M, Rao NC, Gupta N, Vashisth S. Oral health in 4–6 years children with cleft lip/palate: a case control study. North Am J Med Sci. 2014;6(6):266–9.  https://doi.org/10.4103/1947-2714.134371.CrossRefGoogle Scholar
  7. Dahlén G, Preus HR, Baelum V. Methodological issues in the quantification of subgingival microorganisms using the checkerboard technique. J Microbiol Methods. 2015;110:68–77.  https://doi.org/10.1016/j.mimet.2015.01.010.CrossRefPubMedGoogle Scholar
  8. Dewhirst FE, Chen T, Izard J et al. The human oral microbiome. J Bacteriol. 2010;192(19):5002–17.  https://doi.org/10.1128/jb.00542-10.CrossRefPubMedPubMedCentralGoogle Scholar
  9. Ismail AI, Sohn W, Tellez M et al. The International Caries Detection and Assessment System (ICDAS): an integrated system for measuring dental caries. Community Dent Oral Epidemiol. 2007;35(3):170–8.  https://doi.org/10.1111/j.1600-0528.2007.00347.x.CrossRefPubMedGoogle Scholar
  10. Lucas VS, Gupta R, Ololade O, Gelbier M, Roberts GJ. Dental health indices and caries associated microflora in children with unilateral cleft lip and palate. Cleft Palate Craniofac J. 2000;37(5):447–52.CrossRefPubMedGoogle Scholar
  11. Perdikogianni H, Papaioannou W, Nakou M, Oulis C, Papagiannoulis L. Periodontal and microbiological parameters in children and adolescents with cleft lip and/or palate. Int J Paediatr Dent. 2009;19(6):455–67.  https://doi.org/10.1111/j.1365-263X.2009.01020.x.CrossRefPubMedGoogle Scholar
  12. Peterson SN, Snesrud E, Liu J et al. The dental plaque microbiome in health and disease. PLoS One. 2013;8(3):58487.  https://doi.org/10.1371/journal.pone.0058487.CrossRefGoogle Scholar
  13. Quirynen M, Dewinter G, Avontroodt P et al. A split-mouth study on periodontal and microbial parameters in children with complete unilateral cleft lip and palate. J Clin Periodontol. 2003;30(1):49–56 (cpe300108).CrossRefPubMedGoogle Scholar
  14. Rawashdeh MA, Ayesh JA, Darwazeh AM. Oral candidal colonization in cleft patients as a function of age, gender, surgery, type of cleft, and oral health. J Oral Maxillofac Surg. 2011;69(4):1207–13.  https://doi.org/10.1016/j.joms.2010.02.044.CrossRefPubMedGoogle Scholar
  15. Shashni R, Goyal A, Gauba K et al. Comparison of risk indicators of dental caries in children with and without cleft lip and palate deformities. Contemp Clin Dent. 2015;6(1):58–62.  https://doi.org/10.4103/0976-237x.149293.CrossRefPubMedPubMedCentralGoogle Scholar
  16. Socransky SS, Haffajee AD et al. Use of checkerboard DNA-DNA hybridization to study complex microbial ecosystems. Oral Microbiol Immunol. 2004;19(6):352–62.  https://doi.org/10.1111/j.1399-302x.2004.00168.x.CrossRefPubMedGoogle Scholar
  17. Sundell AL, Ullbro C, Marcusson A, Twetman S. Comparing caries risk profiles between 5- and 10- year-old children with cleft lip and/or palate and non-cleft controls. BMC Oral Health. 2015;15:85.  https://doi.org/10.1186/s12903-015-0067-x.CrossRefPubMedPubMedCentralGoogle Scholar
  18. Sundell AL, Nilsson AK, Ullbro C, Twetman S, Marcusson A. Caries prevalence and enamel defects in 5- and 10-year-old children with cleft lip and/or palate: a case-control study. Acta Odontol Scand. 2016;74(2):90–5.  https://doi.org/10.3109/00016357.2015.1044562.CrossRefPubMedGoogle Scholar
  19. Tanner AC, Kressirer CA, Faller LL. Understanding caries from the oral microbiome perspective. J Calif Dent Assoc. 2016;44(7):437–46.PubMedGoogle Scholar
  20. Turesky S, Gilmore ND, Glickman I. Reduced plaque formation by the chloromethyl analogue of victamine C. J Periodontol. 1970;41(1):41–3.CrossRefPubMedGoogle Scholar
  21. Wall-Manning GM, Sissons CH, Anderson SA, Lee M. Checkerboard DNA-DNA hybridisation technology focused on the analysis of Gram-positive cariogenic bacteria. J Microbiol Methods. 2002;51(3):301–11.CrossRefPubMedGoogle Scholar
  22. Weinberg MA, Hassan H. Bleeding on probing: what does it mean? Gen Dent. 2012;60(4):271–6 (quiz page 7–8).PubMedGoogle Scholar

Copyright information

© European Academy of Paediatric Dentistry 2018

Authors and Affiliations

  • A. L. Sundell
    • 1
    Email author
  • C. Ullbro
    • 2
  • G. Dahlén
    • 3
  • A. Marcusson
    • 4
  • S. Twetman
    • 5
  1. 1.Department of Paediatric DentistryInstitute for Postgraduate Dental EducationJönköpingSweden
  2. 2.Department of Clinical DentistryThe Arctic University of NorwayTromsøNorway
  3. 3.Department of Oral Microbiology and ImmunologySahlgrenska Academy at the University of GothenburgGothenburgSweden
  4. 4.Dentofacial Orthopedics, Maxillofacial UnitLinköping University HospitalLinköpingSweden
  5. 5.Department of Odontology, Section for Cariology and Endodontics, Faculty of Health and Medical SciencesUniversity of CopenhagenCopenhagenDenmark

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