European Archives of Paediatric Dentistry

, Volume 17, Issue 4, pp 239–243 | Cite as

Intercomparison of salivary nitric oxide as a biomarker of dental caries risk between caries-active and caries-free children

  • M. SyedEmail author
  • V. Sachdev
  • R. Chopra
Original Scientific Article



To determine the efficacy of salivary nitric oxide as a biomarker for dental caries risk in children and to compare the salivary nitric oxide levels in caries-active and caries-free children.


One hundred children aged 6–12 years were selected and were divided into two groups according to DMFT index: as caries-free consisting of 50 individuals (DMFT/dmft = 0) and caries-active group (DMFT/dmft ≥3) consisting of 50 individuals. The saliva collected was estimated for nitric oxide level by Griess reaction method. Paired t test was used to correlate the difference of mean salivary nitric oxide levels between caries-active and caries-free groups.


The mean level of nitric oxide in saliva of the control group was 581.3 ± 134.6 μg/mL and that of caries-active group was 335.4 ± 111.1 μg/mL, respectively. The mean salivary level of nitric oxide was higher in the caries-free group as compared with the caries-active group which was statistically significant (p = 0.000).


Salivary nitric oxide was higher in the caries-free group as compared to the caries-active group which suggests that increased production of nitric oxide in the oral cavity may contribute to lower caries incidence in children.


Salivary nitric oxide Salivary biomarkers Caries risk assessment 


Compliance with ethical standards

All the procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This study obtained ethical approval from the institutional ethical clearance committee of ITS-CDSR.

Informed consent

Informed consent was obtained from all individual participants included in the study.


This study was not funded by any organisation or institution.

Conflict of interest

All the authors declare that they have no conflict of interest.


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Copyright information

© European Academy of Paediatric Dentistry 2016

Authors and Affiliations

  1. 1.ITS Centre for Dental Studies and ResearchGhaziabadIndia
  2. 2.Department of PedodonticsITS-CDSR Centre for Dental Studies and ResearchGhaziabadIndia

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