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European Archives of Paediatric Dentistry

, Volume 16, Issue 1, pp 9–12 | Cite as

Polymerase chain reaction as a prospect for the early diagnosis and prediction of periodontal diseases in adolescents

  • I. BirsanEmail author
Original Scientific Article

Abstract

Aim

This study was to determine the markers representative of pathogenic periodontal microflora [Prevotella intermedia (P.i), Tannerella forsythia (T.f) [Bacteroides forsythus], Treponema denticola (T.d), Actinobacillus actinomycetemcomitans (A.a), Porphyromonas gingivalis (P.g)] in the dental plaque of adolescents with various degrees of severity of periodontium inflammation.

Methods

Forty-patients aged 15–16 years of age were examined using PMA, CPI and Green–Vermillion indices (Müller 2001). The hygiene status of each patient was also determined using Durr Dental’s Vista Proof intraoral camera (Germany). The polymerase chain reaction (PCR) was performed using a Biometra Thermocycler to detect DNA of pathogenic periodontal bacteria in dental plaque.

Results

All marker microorganisms (P.i. + T.f. + T.d. + A.a. + P.g.) were identified in patients diagnosed with periodontitis in dental plaque. A direct correlation between the level of oral hygiene and the severity of the pathological process in it was determined. It was found that the increase in the severity of the disease was accompanied by increased pathogenic periodontal microflora in dental plaque.

Conclusion

Identification of periodontal pathogens in dental plaque by PCR greatly enhances the early diagnosis of Cronic cattaral gingivitis (CCG) risk factors in adolescents, and allows for detailed analysis of the relation between each factor and severity of the process. This method may be used for the diagnosis of periodontal diseases, prediction of their future course, and reasonable choice of antimicrobial therapy.

Keywords

Pathogenic periodontal microflora Polymerase chain reaction Dental plaque Young adults 

Notes

Acknowledgments

The author expresses her gratitude to the Dep of Paediatric Dentistry and Dental Disease Prevention of A.A. Bogomolets National Medical University and to Prof L.A. Khomenko, to the staff of Diagnostic Laboratory of the Scientific Research Centre of Biological Department of Taras Shevchenko National University of Kiev (Kiev, Ukraine).

References

  1. Amano A, Kuboniwa M, Nakagawa I, et al. Prevalence of specific genotypes of Porphyromonas gingivalis fimA and periodontal health status. J Dent Res. 2000;79(7):1664–8.PubMedCrossRefGoogle Scholar
  2. Donlan RM, Costerton JW. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev. 2002;15(2):167–93.PubMedCentralPubMedCrossRefGoogle Scholar
  3. Greene JC, Vermillion JR. The simplified oral hygiene index. JADA 1964;68:25–31.Google Scholar
  4. Kadowaki T, Nakayama K, Okamoto K, et al. Porphyromonas gingivalis proteinases as virulence determinants in progression of periodontal diseases. J Biochem. 2000;128(2):153–9.PubMedCrossRefGoogle Scholar
  5. Lamont RJ, Burne RA, Lantz MS, LeBlanc DJ. Oral microbiology and immunology. Washington DC: ASM Press; 2010.Google Scholar
  6. Müller H-P. Parodontologie. Stuttgart: Georg Thieme-Verlag; 2001.Google Scholar
  7. Müller H-P, Heinecke A, Zöller L, Fuhrmann A, Eger T. Gingivitis in young adults with Actinobacillus actinomycetemcomitans. Clin Oral Investig. 2001;5(2):83–7.PubMedCrossRefGoogle Scholar
  8. Scardina GA, Buonamente A, Messina P. Efficacy of an oral health training programme for visually impaired individuals. Ig Sanita Publ. 2008;64(4):509–16.Google Scholar
  9. Socransky SS, Haffaiee AD, Cugini MA, Smith C, Kent RL Jr. Microbial complexes in subgingival plaque. J Clin Periodontol. 1998;25(2):134–44.PubMedCrossRefGoogle Scholar

Copyright information

© European Academy of Paediatric Dentistry 2014

Authors and Affiliations

  1. 1.Department of Paediatric Dentistry and Dental Disease PreventionA.A. Bogomolets National Medical UniversityKievUkraine
  2. 2.KievUkraine

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