European Archives of Paediatric Dentistry

, Volume 15, Issue 6, pp 429–433 | Cite as

In vitro toxicity of grey MTA in comparison to white MTA on human periodontal ligament fibroblasts

  • S. N. Al-Haj AliEmail author
  • S. H. Al-Jundi
  • D. J. Ditto
Original Scientific Article



This was to define and compare the in vitro toxicity of grey MTA with that of white MTA on cultured human periodontal ligament (PDL) fibroblasts.


PDL cells were obtained from sound first permanent molars and cultured in Dulbecco’s Modified Eagle’s Medium. Cultures were subjected to different concentrations of grey and white MTA (0.5, 5, 50 and 500 µg/ml) for 24 h at 37 °C. Cells that were not exposed to grey or white MTA served as the negative control. In vitro toxicity was assessed using MTT assay.


The results were compared using ANOVA and Tukey statistical tests (p < 0.05).


White MTA presented higher in vitro toxicity than grey MTA. However, the differences were almost insignificant (p > 0.05).


Both colours of MTA are biocompatible since they were both able to preserve PDL fibroblasts for up to 24 h. MTA is as a promising alternative in pulpotomy of primary teeth.


Grey MTA White MTA Fibroblasts 


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Copyright information

© European Academy of Paediatric Dentistry 2014

Authors and Affiliations

  • S. N. Al-Haj Ali
    • 1
    Email author
  • S. H. Al-Jundi
    • 2
  • D. J. Ditto
    • 2
  1. 1.Department of Orthodontics and Paediatric Dentistry, Faculty of DentistryQassim UniversityQassimKingdom of Saudi Arabia
  2. 2.Preventive Dentistry DepartmentJordan University of Science and TechnologyIrbidJordan

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