In vitro toxicity of grey MTA in comparison to white MTA on human periodontal ligament fibroblasts
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This was to define and compare the in vitro toxicity of grey MTA with that of white MTA on cultured human periodontal ligament (PDL) fibroblasts.
PDL cells were obtained from sound first permanent molars and cultured in Dulbecco’s Modified Eagle’s Medium. Cultures were subjected to different concentrations of grey and white MTA (0.5, 5, 50 and 500 µg/ml) for 24 h at 37 °C. Cells that were not exposed to grey or white MTA served as the negative control. In vitro toxicity was assessed using MTT assay.
The results were compared using ANOVA and Tukey statistical tests (p < 0.05).
White MTA presented higher in vitro toxicity than grey MTA. However, the differences were almost insignificant (p > 0.05).
Both colours of MTA are biocompatible since they were both able to preserve PDL fibroblasts for up to 24 h. MTA is as a promising alternative in pulpotomy of primary teeth.
KeywordsGrey MTA White MTA Fibroblasts
- Camilleri J. A review of the methods used to study biocompatibility of Portland cement-derived materials used in dentistry. MMJ. 2006;18(3):9–13.Google Scholar
- Fallahinejad Ghajari MM, Vatanpour M, Fard M. Comparison of pulpotomy with formocresol and MTA in primary molars: a systematic review and meta-analysis. Int Endod J. 2008;3(3):45–9.Google Scholar
- McDonald RE, Avery DR, Dean JA. Dentistry for the child and adolescent, 9th ed. Mosby; 2011. pp. 343–63.Google Scholar
- Saghiri ML, Daliri M, Aeinehchi M, Behnamghader A. A comparative study of ProRoot MTA and Angelus MTA (in vitro). SRM J Res Dent Sci. 2010;1:60–5.Google Scholar