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Characterization and Analysis of the Skin Microbiota in Rosacea: A Case–Control Study

  • Barbara M. Rainer
  • Katherine G. Thompson
  • Corina Antonescu
  • Liliana Florea
  • Emmanuel F. Mongodin
  • Jonathan Bui
  • Alexander H. Fischer
  • Helena B. Pasieka
  • Luis A. Garza
  • Sewon Kang
  • Anna L. ChienEmail author
Short Communication

Abstract

Background

The efficacy of antibiotics in rosacea treatment suggests a role for microorganisms in its pathophysiology. Growing concern over the adverse effects of antibiotic use presents a need for targeted antimicrobial treatment in rosacea.

Objective

We performed a case–control study to investigate the skin microbiota in patients with rosacea compared to controls matched by age, sex, and race.

Methods

Nineteen participants with rosacea, erythematotelangiectatic, papulopustular, or both, were matched to 19 rosacea-free controls. DNA was extracted from skin swabs of the nose and bilateral cheeks of participants. Sequencing of the V3V4 region of the bacterial 16S ribosomal RNA gene was performed using Illumina MiSeq and analyzed using QIIME/MetaStats 2.0 software.

Results

Compared with controls, skin microbiota in erythematotelangiectatic rosacea was depleted in Roseomonas mucosa (p = 0.004). Papulopustular rosacea was enriched in Campylobacter ureolyticus (p = 0.001), Corynebacterium kroppenstedtii (p = 0.008), and the oral flora Prevotella intermedia (p = 0.001). The highest relative abundance of C. kroppenstedtii was observed in patients with both erythematotelangiectatic and papulopustular rosacea (19.2%), followed by papulopustular (5.06%) and erythematotelangiectatic (1.21%) rosacea. C. kroppenstedtii was also associated with more extensive disease, with the highest relative abundance in rosacea affecting both the cheeks and nose (2.82%), followed by rosacea sparing the nose (1.93%), and controls (0.19%).

Conclusions

The skin microbiota in individuals with rosacea displays changes from that of healthy skin, suggesting that further studies examining a potential role for the skin microbiota in the pathophysiology of rosacea may be warranted.

Notes

Compliance with Ethical Standards

Funding

Research reported in this publication was supported by the National Rosacea Society and the National Institute of Arthritis and Musculoskeletal and Skin Diseases, part of the National Institutes of Health, under R01AR064297 and AR068280 to Luis A. Garza.

Conflict of interest

Anna L. Chien participated in Galderma’s Rosacea Medical Advisory Board Meeting in 2017. Sewon Kang is an advisory board member of Almirall and has received an honorarium. The participation was not in relation to the current article. Barbara M. Rainer, Katherine G. Thompson, Corina Antonescu, Liliana Florea, Emmanuel F. Mongodin, Jonathan Bui, Helena B. Pasieka, Alexander H. Fischer, and Luis A. Garza have no conflicts of interest that are directly relevant to the content of this article.

Ethics approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Johns Hopkins Institutional Review Board and the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Consent to participate

Written informed consent was obtained from all participants included in the study.

Supplementary material

40257_2019_471_MOESM1_ESM.pdf (45 kb)
Supplementary material 1 (PDF 45 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Barbara M. Rainer
    • 1
    • 2
  • Katherine G. Thompson
    • 1
  • Corina Antonescu
    • 3
  • Liliana Florea
    • 3
  • Emmanuel F. Mongodin
    • 4
  • Jonathan Bui
    • 4
  • Alexander H. Fischer
    • 1
  • Helena B. Pasieka
    • 1
  • Luis A. Garza
    • 1
  • Sewon Kang
    • 1
  • Anna L. Chien
    • 1
    Email author
  1. 1.Department of DermatologyJohns Hopkins UniversityBaltimoreUSA
  2. 2.Department of DermatologyMedical University of GrazGrazAustria
  3. 3.McKusick-Nathans Institute of Genetic MedicineJohns Hopkins UniversityBaltimoreUSA
  4. 4.Institute for Genome SciencesUniversity of MarylandBaltimoreUSA

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