Chemical Research in Chinese Universities

, Volume 35, Issue 4, pp 570–576 | Cite as

A Novel Fluorescence Sensor Towards Hydrazine in Living Cells

  • Jinjin Wang
  • Jing Guo
  • Lili Dou
  • Rui Wang
  • Yan Song
  • Qingbiao YangEmail author
  • Jianshi DuEmail author
  • Yaoxian Li


By a simple one-step reaction, a new fluorescein-based probe, 3′-hydroxy-3-oxo-3H-spiro[isobenzofu-ran-1,9′-xanthen]-6′-yl-4-bromobutanoate(FLB), was designed and synthesized. Relative to other cations, anions or amines, the probe exhibited high sensitivity and specificity towards hydrazine with approximate 40-fold fluorescence enhancement at 515 nm. The detection limit(DL) was calculated to be 2.87×10−8 mol/L, which was about 10 times lower than maximum allowable level of hydrazine(3.12×10−7 mol/L) in drinking water permitted by the US Environment and Protection Association(EPA). Simultaneously, through mass spectroscopy and proton nuclear magnetic resonance titration, the detection mechanism was further confirmed. Eventually, due to the low cytotoxicity and excellent water solubility, FLB could be employed to detect hydrazine in living cells.


Fluorescence probe Fluorescein Hydrazine Cell imaging 


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Supplementary material

40242_2019_9109_MOESM1_ESM.pdf (289 kb)
A novel Fluorescence Sensor towards Hydrazine in Living Cells


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Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH 2019

Authors and Affiliations

  1. 1.College of ChemistryJilin UniversityChangchunP. R. China
  2. 2.Jilin Provincial Key Laboratory of Lymphatic SurgeryChina-Japan Union Hospital of Jilin UniversityChangchunP. R. China
  3. 3.College of Materials Science and EngineeringJilin Institute of Chemical TechnologyJilinP. R. China
  4. 4.Hospital Affiliated to Changchun University of Chinese MedicineChangchunP. R. China

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