Chemical Research in Chinese Universities

, Volume 35, Issue 4, pp 581–585 | Cite as

DNA-Silver Nanocluster Binary Probes for Ratiometric Fluorescent Detection of HPV-related DNA

  • Yan Yuan
  • Shaoyuan Li
  • Lei Luo
  • Qing Wang
  • Hongmei Fang
  • Jin Huang
  • Jianbo Liu
  • Xiaohai YangEmail author
  • Kemin WangEmail author


Herein, we described a ratiometric strategy based on “chameleon” DNA-silver nano-clusters(DNA-AgNCs) fluorescent binary probes. The strategy was applied to detect high-risk human papillomavirus(HPV) DNA sequences, HPV-16. First, DNA-AgNCs were synthesized by a simple reduction method. The obtained nanoprobes showed typical yellow and red fluorescence of AgNCs. Upon the addition of HPV-16 DNA, the yellow fluorescence of AgNCs was reduced greatly, whereas the red fluorescence of AgNCs was increased. The concentration of HPV-16 DNA in the samples was characterized by the ratio of fluorescence intensity at 570 and 630 nm. The ratiometric nanoprobes showed good selectivity for HPV-16 DNA, and the detection limit was 2 nmol/L. In addition, the practical applicability of this strategy was demonstrated by analysing the HPV-16 DNA in human serum, illustrating its potential promise for clinical diagnosis.


DNA-silver nanocluster Ratiometric Binary probe Human papillomavirus 


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Supplementary material

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DNA-Silver Nanocluster Binary Probes for Ratiometric Fluorescent Detection of HPV-related DNA


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Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH 2019

Authors and Affiliations

  • Yan Yuan
    • 1
  • Shaoyuan Li
    • 1
  • Lei Luo
    • 1
  • Qing Wang
    • 1
  • Hongmei Fang
    • 1
  • Jin Huang
    • 1
  • Jianbo Liu
    • 1
  • Xiaohai Yang
    • 1
    Email author
  • Kemin Wang
    • 1
    Email author
  1. 1.State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-nanotechnology and Molecular Engineering of Hunan ProvinceHunan UniversityChangshaP. R. China

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