Spectral Study on the Interactions Among Cu(II), Doxorubicin and CopC
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Interactions among Cu(II), doxorubicin and CopC have been investigated in detail by means of fluorescence, UV-Vis, IR spectra, isothermal titration calorimetry(ITC) and molecular docking in Tris-HCl buffer(50 mmol/L, pH=7.4, 25 °C). The results suggest that Cu(II)-doxorubicin is formed in a Cu(II) to doxorubicin ratio of 1:2, and the conditional stability constant, K[Cu(II)-doxorubicin] is 1.90×109 L2/mol2, CopC and doxorubicin can form a 1:1 complex, the conditional stability constant is greater than 105 L/mol. Binding of doxorubicin causes a conformational change in CopC with the reduction of β-sheet and increase of random coil, and the stability of CopC is decreased. Cu(II), doxorubicin and CopC can form a CopC-Cu(II)-doxorubicin ternary complex. The formation of CopC-Cu(II)- doxorubicin reduced greatly the reduction rate of Cu(II) by ascorbate(Vc), i.e. the binding of doxorubicin affects the action of CopC as redox switch.
KeywordsDoxorubicin CopC Cu(II) Interaction
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Thanks to Large-scale Scientific Instrument Center of Shanxi University for the use of Isothermal Titration Calorimetry 200.
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