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Spectral Study on the Interactions Among Cu(II), Doxorubicin and CopC

  • Yunxi Song
  • Zhen Song
  • Binsheng YangEmail author
Article
  • 7 Downloads

Abstract

Interactions among Cu(II), doxorubicin and CopC have been investigated in detail by means of fluorescence, UV-Vis, IR spectra, isothermal titration calorimetry(ITC) and molecular docking in Tris-HCl buffer(50 mmol/L, pH=7.4, 25 °C). The results suggest that Cu(II)-doxorubicin is formed in a Cu(II) to doxorubicin ratio of 1:2, and the conditional stability constant, K[Cu(II)-doxorubicin] is 1.90×109 L2/mol2, CopC and doxorubicin can form a 1:1 complex, the conditional stability constant is greater than 105 L/mol. Binding of doxorubicin causes a conformational change in CopC with the reduction of β-sheet and increase of random coil, and the stability of CopC is decreased. Cu(II), doxorubicin and CopC can form a CopC-Cu(II)-doxorubicin ternary complex. The formation of CopC-Cu(II)- doxorubicin reduced greatly the reduction rate of Cu(II) by ascorbate(Vc), i.e. the binding of doxorubicin affects the action of CopC as redox switch.

Keywords

Doxorubicin CopC Cu(II) Interaction 

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Notes

Acknowledgements

Thanks to Large-scale Scientific Instrument Center of Shanxi University for the use of Isothermal Titration Calorimetry 200.

Supplementary material

40242_2019_8284_MOESM1_ESM.pdf (512 kb)
Spectral study on the interaction between doxorubicin and CopC

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Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular ScienceShanxi UniversityTaiyuanP. R. China
  2. 2.Department of ChemistryTaiyuan Normal UniversityJinzhongP. R. China

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