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Artificial Esterase Based on Self-assembly Gel Microspheres Constructed from Chitosan and Amino Acids

  • Jing Cao
  • Miao WangEmail author
  • Weihua Chen
  • Yongxin She
  • Jing WangEmail author
  • Fengzhong Wang
  • Shuibing Lao
Article
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Abstract

A novel artificial esterase based on chitosan and amino acids was synthesized in the present study. The Fmoc-His and Glu were linked to chitosan by active ester method(AEM). The hydroxide radical in chitosan, imidazole group of Fmoc-His and carboxyl from Glu formed a catalytic center of natural esterase. Gel microspheres were coated with a protective layer and a supporting layer by self-assembly construction function in carboxymethylcellulose sodium(CMCS) solution. As for catalytic activity, chitosan-His-Glu was found to be more efficient than chitosan- His and chitosan-Glu in mimicking the core catalytic sites of natural esterase, and the best ratio(mass ratio) of chitosan-His-Glu:CMCS was 1:3. Furthermore, metal ions, such as Ca2+, Mg2+, Fe2+, etc., were able to improve the catalytic efficiency of artificial esterase. And the Lineweaver-Burk plot indicated that the catalytic kinetics of artificial esterase conformed to Michaelis-Menten equation.

Keywords

Artificial enzyme Esterase Chitosan 

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Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH 2019

Authors and Affiliations

  1. 1.Institute of Quality Standardization & Testing Technology for Agro-productsChinese Academy of Agricultural SciencesBeijingP. R. China
  2. 2.Key Laboratory of Agrofood Safety and Quality(Beijing)Ministry of AgricultureBeijingP. R. China
  3. 3.Institute of Agro-products Processing Science and TechnologyChinese Academy of Agricultural SciencesBeijingP. R. China
  4. 4.Agro-products Quality Safety and Testing Technology Research InstituteGuangxi Academy of Agricultural SciencesNanningP. R. China

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