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Chemical Research in Chinese Universities

, Volume 34, Issue 6, pp 887–892 | Cite as

Determination of Lignans in Schisandra sphenanthera and Schisandra chinensis Using Ionic Liquid-based Ultrasonic-assisted Extraction and High-performance Liquid Chromatography

  • Lianyue Guan
  • Qiang Luo
  • Na Liang
  • Wei YuEmail author
Article
  • 17 Downloads

Abstract

A green, rapid and precise sample pretreatment technique, IL-based UAE(ionic liquid-based ultrasonic-assisted extraction), was coupled with high-performance liquid chromatographic separation to identify the main effective components in Schisandra sphenanthera(S. sphenanthera) and Schisandra chinensis(S. chinensis) including schisantherin A, schisandrin A, and deoxyschizandrin. Four different types of ionic liquids have been investigated, finally [C6MIM][BF4] was used as the extraction solvent. A powder form of S. sphenanthera and S. chinensis was mixed with the [C6MIM][BF4] to produce a suspension. This suspension was ultrasonically extracted in a water bath at room temperature. Several of the process parameters were optimized, including the type of ionic liquid used and its volume, the sample amount, the size of the sample particle, the extraction time, etc. HPLC calibration curves were established for all the analytes and proved to be linear(r>0.9999). The lowest detection level for schisandrin A was 0.12 μg/mL, for schisantherin A was 0.08 μg/mL, and for deoxyschizandrin was 0.10 μg/mL. The recoveries of the target compounds were from 74.19% to 109.33%. The standard deviations for detection were generally no more than 6.31%. In contrast to conventional extraction methods, the IL-based UAE did not involve volatile organic volatile solvents, and the analysis time, required sample and solvent volumes were also lower than those of the conventional techniques.

Keywords

Ionic liquid High-performance liquid chromatography Ultrasonic-assisted extraction Effective constituent 

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Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.China-Japan Union HospitalJilin UniversityChangchunP. R. China
  2. 2.Office of Surgical NursingChangchun Medical CollegeChangchunP. R. China

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