Advertisement

Expression of Phytoestrogens in pGL2/AQP1 Promoter Reporter System

  • Wei Wei
  • Chaoyue Zhao
  • Sitong Liu
  • Xiaofei Jiao
  • Xintong Liu
  • Chuanjian Lan
  • Jiang Li
Article

Abstract

In this study, we amplified aquaporin 1(AQP1) promoter sequence with polymerase chain reaction(PCR), then AQP1 promoter fragment and pGL2 basic vector were linked to create an artificial pGL2/AQP1 promoter re-porter system. A certain concentration of 17β-estradiol(E2) activated pGL2/AQP1p, which demonstrated the pGL2/AQP1p transcriptional system effective. The pGL2/AQP1 promoter reporter system was applied to evaluate the activate effect on AQP1 of different kinds of phytoestrogens. Dual luciferase reporter gene activity assay showed that a certain concentration phytoestrogens including daidzein and genistein can increase AQP1 promoter transcription activity. In addition, E2, daidzein and genistein can make AQP1 protein endoge- nous expression level increase and promote the function of water scretion. The result can guide the clinical application to treat the Sjogren’s syndrome and provide a necessary molecular tool for the subsequent drug screening.

Keywords

Aquaporin 1(AQP1) Sjogren’s syndrome 17β-Estradiol Phytoestrogen 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    Delaleu N., Jonsson R., Koller M. M., European Journal of Oral Sciences, 2005, 113(2), 101CrossRefGoogle Scholar
  2. [2]
    Hammi A. R., Al-Hashimi I. H., Nunn M. E., Zipp M., Journal of Oral Pathology and Medicine, 2005, 34(4), 198CrossRefGoogle Scholar
  3. [3]
    Christine D., Angelic B., Jason P., International Journal of Molecu-lar Sciences, 2016, 17(2), 166CrossRefGoogle Scholar
  4. [4]
    Beroukas D., Hiscock J., Gannon B. J., Jonsson R., Gordon T. P., Waterman S. A., Laboratory Investigation, 2002, 82(11), 1547CrossRefGoogle Scholar
  5. [5]
    Enger T. B., Aure M. H., Jensen J. L., Galtung H. K., Acta Odonto-logica Scandinavica, 2014, 72(7), 549CrossRefGoogle Scholar
  6. [6]
    Wang Z., Zourelias L., Wu C., Edwards P. C., Trombetta M., Gene Therapy, 2015, 22(9), 739CrossRefGoogle Scholar
  7. [7]
    Smith B. L., Agre P., Journal of Biological Chemistry, 1991, 266(10), 6407Google Scholar
  8. [8]
    Preston G. M., Carroll T. P., Guggino W. B., Agre P., Science, 1992, 256(5055), 385CrossRefGoogle Scholar
  9. [9]
    Verbavatz J. M., Brown D., Sabolic I., Valenti G., Journal of Cell Biology, 1993, 123(3), 605CrossRefGoogle Scholar
  10. [10]
    Jung J. S., Preston G. M., Smith B. L., Guggino W. B., Agre P., Journal of Biological Chemistry, 1994, 269(20), 14648Google Scholar
  11. [11]
    Gresz V., Kwon T. H., Hurley P. T., Varga G., Zelles T., Nielsen S., Case R. M., Steward M. C., American Journal of Physiolo-gy-Gastrointestinal and Liver Physiology, 2001, 281(1), 247CrossRefGoogle Scholar
  12. [12]
    Fischbarg J., Physiological Reviews, 2010, 90(4), 1271CrossRefGoogle Scholar
  13. [13]
    Bruce J. I., Giovannucci D. R., Blinder G., Shuttleworth T. J., Yule D. I., Journal of Biological Chemistry, 2004, 279(13), 12909CrossRefGoogle Scholar
  14. [14]
    Hayashi T., Journal of Biomedicine and Biotechnology, 2011, 2011, 407031CrossRefGoogle Scholar
  15. [15]
    Liu S., Niu K., Da Y., Liu Y., Zhang J., Wang W., Zhang Y., Jiang H., Wang K., Bai W., Qin L., Jia J., Biomedicine and Pharmacotherapy, 2018, 97, 1438CrossRefGoogle Scholar
  16. [16]
    Lago M. L., de Oliveira A. E., Lopes F. F., Ferreira E. B., Rodrigues V. P., Gynecological Endocrinology, 2015, 31(2), 109CrossRefGoogle Scholar
  17. [17]
    Kininis M., Chen B. S., Diehl A. G., Isaacs G. D., Zhang T., Siepel A. C., Molecular and Cellular Biology, 2007, 27(14), 5090CrossRefGoogle Scholar
  18. [18]
    Davis S. R., Dalais F. S., Simpson E. R., Murkies A. L., Recent Progress in Hormone Research, 1999, 54, 185Google Scholar
  19. [19]
    Rybas Y. A., Gonzáles K. F., Kravtsov E. G., Tchernitchin A. N., Da-lin M. V., Bulletin of Experimental Biology & Medicine, 2011, 152(1), 83CrossRefGoogle Scholar
  20. [20]
    Porola P., Laine M., Virkki L., Poduval P., Konttinen Y. T., Annals of The New York Academy of Sciences, 2007, 1108(1), 426CrossRefGoogle Scholar
  21. [21]
    Giuca M. R., Carli E., Pasini M., Bonfigli D., Cappe M. R., Minerva Ginecologica, 2009, 61(1), 13Google Scholar
  22. [22]
    Ciotta L., Stracquadanio M., Pagano I., Andò A., Valenti O., Rocca-salva L., Minerva Ginecological, 2012, 64(1), 15Google Scholar

Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Wei Wei
    • 1
  • Chaoyue Zhao
    • 1
    • 2
  • Sitong Liu
    • 3
  • Xiaofei Jiao
    • 1
  • Xintong Liu
    • 1
  • Chuanjian Lan
    • 1
  • Jiang Li
    • 1
  1. 1.Dental Hospital of Jilin UniversityChangchunP. R. China
  2. 2.The First Hospital of HarbinHarbinP. R. China
  3. 3.Edmond H. Fischer Signal Transduction Laboratory, School of Life SciencesJilin UniversityChangchunP. R. China

Personalised recommendations