Chemical Research in Chinese Universities

, Volume 34, Issue 6, pp 929–933 | Cite as

Expression of Phytoestrogens in pGL2/AQP1 Promoter Reporter System

  • Wei Wei
  • Chaoyue Zhao
  • Sitong Liu
  • Xiaofei Jiao
  • Xintong Liu
  • Chuanjian Lan
  • Jiang Li


In this study, we amplified aquaporin 1(AQP1) promoter sequence with polymerase chain reaction(PCR), then AQP1 promoter fragment and pGL2 basic vector were linked to create an artificial pGL2/AQP1 promoter re-porter system. A certain concentration of 17β-estradiol(E2) activated pGL2/AQP1p, which demonstrated the pGL2/AQP1p transcriptional system effective. The pGL2/AQP1 promoter reporter system was applied to evaluate the activate effect on AQP1 of different kinds of phytoestrogens. Dual luciferase reporter gene activity assay showed that a certain concentration phytoestrogens including daidzein and genistein can increase AQP1 promoter transcription activity. In addition, E2, daidzein and genistein can make AQP1 protein endoge- nous expression level increase and promote the function of water scretion. The result can guide the clinical application to treat the Sjogren’s syndrome and provide a necessary molecular tool for the subsequent drug screening.


Aquaporin 1(AQP1) Sjogren’s syndrome 17β-Estradiol Phytoestrogen 


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Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Wei Wei
    • 1
  • Chaoyue Zhao
    • 1
    • 2
  • Sitong Liu
    • 3
  • Xiaofei Jiao
    • 1
  • Xintong Liu
    • 1
  • Chuanjian Lan
    • 1
  • Jiang Li
    • 1
  1. 1.Dental Hospital of Jilin UniversityChangchunP. R. China
  2. 2.The First Hospital of HarbinHarbinP. R. China
  3. 3.Edmond H. Fischer Signal Transduction Laboratory, School of Life SciencesJilin UniversityChangchunP. R. China

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