A Label-free and Functional Fluorescent Oligonucleotide Probe Based on a G-Quadruplex Molecular Beacon for the Detection of Kanamycin
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A label-free and turn-off fluorescent method for the quantitative detection of kanamycin based on a functional molecular beacon was developed. The molecular beacon consists of two hairpin structures with a split G-rich oligonucleotide in the middle. The kanamycin’s aptamer formed the loops portion for recognizing kanamycin, and the G-quadruplex bound by Thioflavin T(ThT) was employed as the reporter. In the absence of target, the molecular beacon folded into double stem-loops and the splited G-rich oligonucleotid came close to form a G-quadruplex. When ThT bound to the G-quadruplex, the fluorescence intensity of the solution increased. Upon the addition of kanamycin, the function between kanamycin and aptamer unfolded the hairpin and disassembled the G-quadraplex structure, resulting in a significant decrease in the fluorescence intensity. A good linear relationship ranging from 0.7 nmol/L to 10 nmol/L was achieved and the limit of detection was 0.37 nmol/L. Besides, it could efficiently recognize kanamycin in real samples.
KeywordsMolecular beacon Fluorescence Kanamycin G-Quadruplex Thioflavin T
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- Long Y. H., Hernandez M., Kaale E., van Schepdael R. A., E., Bor-rull F., Calull M., Hoogmartens J., J. Chromatogr., 2003, 784, 255Google Scholar
- Tyagi S., Kramer N. F. R., Biotechnol., 1996, 14, 303Google Scholar
- Liu B., Yang X., Wang K., Chem. Res. Chinese Universities, 2012, 28(1), 37Google Scholar
- Wen Y., Wang L., Li L., Sensors, 2016, 16, 12Google Scholar
- Stoltenburg R., Nikolaus N., Strehlitz B., J. Anal. Methods Chem., 2012, 14, 383Google Scholar
- Green J., Ying L., Klenerman D., Chem. Res. Chinese Universities, 2002, 18(1), 103Google Scholar