The effect of Nigella sativa oil on serum levels of inflammatory markers, liver enzymes, lipid profile, insulin and fasting blood sugar in patients with non-alcoholic fatty liver
Non-alcoholic fatty liver disease (NAFLD) is one of the metabolic disturbances associated with inflammation. Nigella sativa (NS) seed oil has different chemical compounds including Thymoquinone (TQ), unsaturated fatty acids, and flavonoids. NSs are used as anti-inflammatory and antioxidants in medical sciences. This study aimed to investigate the effect of NS oil on several parameters in serum levels of patients with NAFLD.
Forty-four patients diagnosed with NAFLD participated in a randomized, double-blind, placebo-controlled clinical trial. Patients were randomly assigned into two groups; one receiving NS oil and the other receiving placebo (paraffin oil), for 8 weeks. Blood samples were taken from the patients at the beginning and the end of the study. Afterwards, liver enzymes (ALT, AST, and GGT), inflammatory markers (Hs-CRP, TNF-α, and IL-6), insulin, lipid profiles (total cholesterol, triglyceride, VLDL, LDL-C, and HDL-C), FBS, and blood pressure were measured.
Consumption of NS seed oil as supplement decreased the FBS level, lipid profiles (TG, TC, LDL, VLDL), liver enzymes (AST and ALT), hs-CRP inflammatory marker, IL-6, TNF-α, while it increased the HDL-C levels, compared to the placebo group (P < 0.05). Receiving NS oil had no significant effect on serum levels of insulin, blood pressure, and GGT in comparison with the beginning of the study (P < 0.05).
NS seed oil supplements may decrease the liver enzymes and lipid profiles in the patients with NAFLD and play a protective role in the liver via reducing the inflammation in this group of patients.
KeywordsNigella sativa NAFLD Insulin Liver enzymes Inflammation
body mass index
High density lipoprotein cholesterol
Low-density lipoprotein cholesterol
very low-density lipoprotein cholesterol
tumor necrosis factor-a
high sensitivity C-reactive protein.
This article was extracted from the Master’s thesis of Mohammad Rashidmayvan (student at Ahvaz Jundishapur University of Medical Sciences).
All authors participated in the design, study, data acquisition and drafting manuscript. All authors read and approved the final manuscript.
The financial support of Ahvaz Jundishapur University of Medical Sciences is highly appreciated.
Compliance with ethical standards
Ethics approval and consent to participate
Approved by Institutional Ethical Committee of Jundishapur University of Medical Sciences, Ahvaz, Iran.: IR.AJUMS.REC.1395.695.
Consent for publication
The authors declare that they have no competing interests.
- 6.Khoshbaten M. Comparison character of clinical and laboratory of nonalcoholic fatty liver disease with healthy people. J Tabib Shargh Sci; 2009. P: 13-21.] Persian.Google Scholar
- 7.Jou J, Choi SS, Diehl AM, editors. Mechanisms of disease progression in nonalcoholic fatty liver disease2008.Google Scholar
- 17.Mansour MA, Ginawi OT, El-Hadiyah T, El-Khatib AS, Al-Shabanah OA, Al-Sawaf HA. Effects of volatile oil constituents of Nigella sativa on carbon tetrachloride-induced hepatotoxicity in mice: evidence for antioxidant effects of thymoquinone. Res Commun Mol Pathol Pharmacol. 2001;110(3–4):239–51.Google Scholar
- 20.Hussain M, Tunio AG, Arain LA, Shaikh GS. Effects of nigella sativa on various parameters in patients of non-alcoholic fatty liver disease. J Ayub Med Coll Abbottabad. 2017;29(3):403–7.Google Scholar
- 21.Najmi A, Haque SF, Naseeruddin M, Khan RA. Effect of Nigella sativa oil on various clinical and biochemical parameters of metabolic syndrome. Int J Diabetes Dev Ctries. 2008;16:85–7.Google Scholar
- 24.Al-Naqeep G, Ismail M, Allaudin Z. Regulation of low-density lipoproteinreceptor and 3-hydroxy-3- methylglutaryl coenzyme a reductase geneexpression by thymoquinone-rich fraction and thymoquinone in HepG2cells. J Nutrigenet Nutrigenomics. 2010;2(4–5):163–72.Google Scholar
- 28.Bamosa AO, Ali BA, Sowayan SA. Effect of oral ingestion of Nigella sativaseeds on some blood parameters. Saudi Pharm J. 1997;5(2–3):126–9.Google Scholar
- 39.Bamosa AO, Kaatabi H, Lebdaa FM, Elq AM, Al-Sultanb A. Effect of Nigella sativa seeds on the glycemic control of patients with type 2 diabetes mellitus. Indian J Physiol Pharmacol. 2010;54(4):344–54.Google Scholar