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Microwave assisted synthesis of polyacrylamide grafted polymeric blend of fenugreek seed mucilage-Polyvinyl alcohol (FSM-PVA-g-PAM) and its characterizations as tissue engineered scaffold and as a drug delivery device

  • Trishna BalEmail author
  • Sabyasachi Swain
Research Article
  • 12 Downloads

Abstract

Microwave assisted synthesis of graft copolymer of polymeric blend of Fenugreek seed mucilage (FSM)-Polyvinyl alcohol (PVA) with acrylamide (AM) was done by free radical polymerization using ammonium per sulfate (APS) as initiator. Varying amount of AM and APS was used to optimize the best grade based on highest percentage grafting efficiency and investigated with intrinsic viscosity measurement, Fourier Transformation infrared spectroscopy (FTIR),13C NMR spectra, X-ray diffraction, elemental analysis, Thermogravimetric analysis, Scanning electron microscopy. The results of intrinsic viscosity indicate that the optimized sample GF4 has longer chain length than in comparison to the native mucilage and thus exhibits more swelling tendencies and thus can be used as very good controlled release matrix system. The thermal analysis and X-ray indicates that GF4 is more stable and possess more amorphous properties than the native FSM. The NMR and FT-IR studies reveal that in GF4 there is prominent presence of amide and the hydroxyl groups indicating that grafting mechanism has efficiently taken place. Histological studies & SEM image for optimized grade implanted on animals revealed sufficient tissue growth and exhibited biodegradability proving the material to be biocompatible and suitable to be used as tissue engineered scaffolds. The controlled release behavior of the optimized polymeric system GF4 was evidenced by 95% release of loaded drug Enalapril maleate for 16 h.

Graphical abstract

Keywords

Graft copolymer Metronidazole Histopathology Scaffold Fenugreek seed mucilage 

Notes

Acknowledgments

The authors acknowledge the instrumental support of Central Instrumentation Facility, Birla Institute of Technology, Mesra, for sophisticated instrument to carry out this experiment.

Author’s contributions

The experimental study was done by Corresponding author Trishna Bal (TB) as a part of her reserach work who did all the practical experiments and drafted the article. The other coauthor, Sabyasachi Swain (SS) did the insertion of the polymeric scaffolds in animals. All authors read and approved the final copy of the text.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Supplementary material

40199_2019_237_MOESM1_ESM.docx (181 kb)
ESM 1 (DOCX 181 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Pharmaceutical Sciences and TechnologyBirla Institute of Technology MesraRanchiIndia

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