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Acta Metallurgica Sinica (English Letters)

, Volume 32, Issue 10, pp 1253–1260 | Cite as

Inducing Macrophages M2 Polarization by Dexamethasone Laden Mesoporous Silica Nanoparticles from Titanium Implant Surface for Enhanced Osteogenesis

  • Jing Luo
  • Xin Ding
  • Wen SongEmail author
  • Jian-Ying Bai
  • Jing Liu
  • Zhe Li
  • Fan-Hui Meng
  • Fang-Hao Chen
  • Yu-Mei ZhangEmail author
Article
  • 38 Downloads

Abstract

The study conveys an idea to enhance the osseointegration of titanium implant (Ti) through modulating macrophages M2 polarization. The ~ 100 nm spherical mesoporous silica nanoparticles (MSN) that compromised of ~ 4-nm-diameter nano-tunnels were synthesized by the conventional “sol–gel” method, into which the dexamethasone (DEX) was loaded (DEX@MSN). The DEX@MSN could consistently release DEX and showed favorable cytocompatibility in RAW264.7 cells. The arginase-1 expression, a specific marker for macrophages M2 polarization, was also enhanced by DEX@MSN treatment. Then, the Ti was pre-treated with anodization under 5 V to generate the titania nanotubes with ~ 30 nm diameter (NT-30) and the DEX@MSN was introduced onto NT-30 surface via electrophoretic deposition, with the aid of chitosan. After optimizing the deposition parameters, the supernatants of RAW264.7 from the decorated implant surface could significantly promote the osteogenic differentiation of murine primary bone marrow mesenchymal stem cells. These findings demonstrate that delivery of DEX from implant surface can modulate the macrophages M2 polarization and result in favorable osteogenesis.

Keywords

Macrophages polarization Titanium implant Osteogenesis Dexamethasone Mesoporous silica nanoparticles Electrophoretic deposition 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 31800790, 81530051 and 31670966).

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Copyright information

© The Chinese Society for Metals (CSM) and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Jing Luo
    • 1
    • 2
  • Xin Ding
    • 3
  • Wen Song
    • 1
    Email author
  • Jian-Ying Bai
    • 1
  • Jing Liu
    • 1
  • Zhe Li
    • 1
  • Fan-Hui Meng
    • 4
  • Fang-Hao Chen
    • 1
  • Yu-Mei Zhang
    • 1
    Email author
  1. 1.State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of StomatologyThe Fourth Military Medical UniversityXi’anChina
  2. 2.Department of StomatologyGeneral Hospital of Southern Theater Command of the Chinese People’s Liberation ArmyGuangzhouChina
  3. 3.Huaian Stomatological HospitalHuaianChina
  4. 4.State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Key Laboratory of Oral Diseases, Department of Dental Materials, School of StomatologyThe Fourth Military Medical UniversityXi’anChina

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