High-Resolution Microscopy for Imaging Cancer Pathobiology
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Purpose of Review
Light microscopy plays an essential role in clinical diagnosis and understanding the pathogenesis of cancer. Conventional bright-field microscope is used to visualize abnormality in tissue architecture and nuclear morphology, but often suffers from many limitations. This review focuses on the potential of new imaging techniques to improve basic and clinical research in pathobiology.
Light microscopy has significantly expanded its ability in resolution, imaging volume, speed, and contrast. It now allows 3D high-resolution volumetric imaging of tissue architecture from large tissue and molecular structures at nanometer resolution.
Pathologists and researchers now have access to various imaging tools to study cancer pathobiology in both breadth and depth. Although clinical adoption of a new technique is slow, the new imaging tools will provide significant new insights and open new avenues for improving early cancer detection and personalized risk assessment and identifying the best treatment strategies.
KeywordsLight microscopy 3D volumetric imaging Super-resolution microscopy Label-free imaging
Due to the large body of literatures, we cannot cover all of the related topics and publications. We acknowledge Dr. Hongbin Ma for preparing Fig. 2d. We apologize to researchers whose work is missed in this review.
We acknowledge the funding support from National Institute of Health Grant Numbers R01CA185363 and R33CA225494.
Compliance With Ethical Standards
Conflict of Interest
Jianquan Xu declares no conflict of interest. Yang Liu is the co-inventor for several US patents on light microscopy techniques to analyze nanoscale nuclear architecture for cancer diagnosis and other applications, owned by the University of Pittsburgh.
Human and Animal Rights and Informed Consent
This article does not contain any studies with human or animal subjects performed by any of the authors.
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