Analysis of mtCOI and 18S rRNA Sequence-Based Characterization of Recently Commercialized Marine Edible Pufferfishes

  • Karunanidhi Kaleshkumar
  • Rajendran RajaramEmail author
Research Article


The pufferfishes are considered as a serious hazard to consumers since it contains a strong marine toxin which can be lethal to human beings. However, some species of the pufferfishes have commercial value in the food industry and in the aquarium trade. Hence, the identification of pufferfishes plays a major role to find out their type. Analysis of genetic materials is the constitutional source of rapid and accurate species identification method to reveal even intra-specific difference of their phenotypic plasticity when morphological distinctiveness fails to provide intact information. In the present study, twenty marine pufferfish specimens belonging to five different commercially important species were collected from April 2016 to May 2017 from Mandapam region, east coast of Tamil Nadu. Consensus primers of two different genes (mtCOI and 18S rRNA) were employed to compare the genetic variations. Results suggest that the species within the taxa were accurately identified by mtCOI gene when compared to the 18S rRNA gene. Further, the computational analytical tools such as multiple sequence alignment, genetic distance analysis, phylogenetic analysis and motifs were used to study the genetic variation between the species. This investigation recommends the accurate identification of pufferfishes using genetic marker since these species are recently commercialized for human consumption.


Phylogenetic analysis Genetic distance analysis Motif enrichment Regular expression pattern DNA barcoding 



The authors would like to thank the authorities of Bharathidasan University for providing the desired facilities for this research. They also thank University Grants Commission/Rajiv Gandhi National Fellowship (UGC/RGNF), New Delhi, Government of India, for financial assistance.


This work was partly funded by the University Grants Commission/Rajiv Gandhi National Fellowship (UGC/RGNF), New Delhi, Government of India (F1-17.1/2016-17/RGNF-2015-17-SC-TAM-19298/(SA-III Web site).

Compliance with ethical standards

Conflict of Interest

The authors declare that they have no conflict of interest to publish this manuscript.


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Copyright information

© The National Academy of Sciences, India 2019

Authors and Affiliations

  1. 1.DNA Barcoding and Marine Genomics Laboratory, Department of Marine Science, School of Marine SciencesBharathidasan UniversityTiruchirappalliIndia

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